Sword Bean (<i>Canavalia gladiata</i>) Pods Induce Differentiation in MC3T3-E1 Osteoblast Cells by Activating the BMP2/SMAD/RUNX2 Pathway
Sword bean (SB) contains various phytochemicals, such as flavonoids, tannins, saponins, and terpenoids. Although the evaluation of its potential functions, including antioxidant, anti-obesity, anti-inflammatory, liver protection, and antiangiogenic activities, has been widely reported, research on t...
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MDPI AG
2023-10-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/20/4372 |
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| author | Yu Jin Hwang Hye-Jeong Hwang Hyunseo Go NaYeong Park Kyung-A Hwang |
| author_facet | Yu Jin Hwang Hye-Jeong Hwang Hyunseo Go NaYeong Park Kyung-A Hwang |
| author_sort | Yu Jin Hwang |
| collection | DOAJ |
| description | Sword bean (SB) contains various phytochemicals, such as flavonoids, tannins, saponins, and terpenoids. Although the evaluation of its potential functions, including antioxidant, anti-obesity, anti-inflammatory, liver protection, and antiangiogenic activities, has been widely reported, research on their use in osteoporosis prevention is insufficient. Furthermore, while various studies are conducted on SB, research on sword bean pods (SBP) is not yet active, and little is known about it. Therefore, this study investigated the effects of promoting osteoblast differentiation of MC3T3-E1 cells using SB and SBP extracts and their mechanisms. We show that SBP extracts increase osteoblast proliferation, mineralization-activated alkaline phosphatase (ALP), and collagen synthesis activities. Additionally, treatment with SBP extract increased the expression of markers related to osteoblast differentiation, such as ALP, SPARC, RUNX2, COL-I, BMP2, OCN, and OPN. It was confirmed that SBP induces differentiation by activating the BMP2/SMAD/RUNX2 pathway. We also show that SBP is more effective than SB, and SBP may be useful in assimilating bone minerals and preventing osteoporosis. |
| first_indexed | 2024-03-10T20:59:57Z |
| format | Article |
| id | doaj.art-b9cbf85f64004ecabd3e1e1ad01624ec |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T20:59:57Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-b9cbf85f64004ecabd3e1e1ad01624ec2023-11-19T17:37:52ZengMDPI AGNutrients2072-66432023-10-011520437210.3390/nu15204372Sword Bean (<i>Canavalia gladiata</i>) Pods Induce Differentiation in MC3T3-E1 Osteoblast Cells by Activating the BMP2/SMAD/RUNX2 PathwayYu Jin Hwang0Hye-Jeong Hwang1Hyunseo Go2NaYeong Park3Kyung-A Hwang4Department of Agrofood Resources, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun 55365, Republic of KoreaDepartment of Agrofood Resources, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun 55365, Republic of KoreaDepartment of Agrofood Resources, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun 55365, Republic of KoreaDepartment of Agrofood Resources, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun 55365, Republic of KoreaDepartment of Agrofood Resources, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun 55365, Republic of KoreaSword bean (SB) contains various phytochemicals, such as flavonoids, tannins, saponins, and terpenoids. Although the evaluation of its potential functions, including antioxidant, anti-obesity, anti-inflammatory, liver protection, and antiangiogenic activities, has been widely reported, research on their use in osteoporosis prevention is insufficient. Furthermore, while various studies are conducted on SB, research on sword bean pods (SBP) is not yet active, and little is known about it. Therefore, this study investigated the effects of promoting osteoblast differentiation of MC3T3-E1 cells using SB and SBP extracts and their mechanisms. We show that SBP extracts increase osteoblast proliferation, mineralization-activated alkaline phosphatase (ALP), and collagen synthesis activities. Additionally, treatment with SBP extract increased the expression of markers related to osteoblast differentiation, such as ALP, SPARC, RUNX2, COL-I, BMP2, OCN, and OPN. It was confirmed that SBP induces differentiation by activating the BMP2/SMAD/RUNX2 pathway. We also show that SBP is more effective than SB, and SBP may be useful in assimilating bone minerals and preventing osteoporosis.https://www.mdpi.com/2072-6643/15/20/4372sword bean podsMC3T3-E1 cellsosteoblastBMP2/SMAD/RUNX2 pathway |
| spellingShingle | Yu Jin Hwang Hye-Jeong Hwang Hyunseo Go NaYeong Park Kyung-A Hwang Sword Bean (<i>Canavalia gladiata</i>) Pods Induce Differentiation in MC3T3-E1 Osteoblast Cells by Activating the BMP2/SMAD/RUNX2 Pathway Nutrients sword bean pods MC3T3-E1 cells osteoblast BMP2/SMAD/RUNX2 pathway |
| title | Sword Bean (<i>Canavalia gladiata</i>) Pods Induce Differentiation in MC3T3-E1 Osteoblast Cells by Activating the BMP2/SMAD/RUNX2 Pathway |
| title_full | Sword Bean (<i>Canavalia gladiata</i>) Pods Induce Differentiation in MC3T3-E1 Osteoblast Cells by Activating the BMP2/SMAD/RUNX2 Pathway |
| title_fullStr | Sword Bean (<i>Canavalia gladiata</i>) Pods Induce Differentiation in MC3T3-E1 Osteoblast Cells by Activating the BMP2/SMAD/RUNX2 Pathway |
| title_full_unstemmed | Sword Bean (<i>Canavalia gladiata</i>) Pods Induce Differentiation in MC3T3-E1 Osteoblast Cells by Activating the BMP2/SMAD/RUNX2 Pathway |
| title_short | Sword Bean (<i>Canavalia gladiata</i>) Pods Induce Differentiation in MC3T3-E1 Osteoblast Cells by Activating the BMP2/SMAD/RUNX2 Pathway |
| title_sort | sword bean i canavalia gladiata i pods induce differentiation in mc3t3 e1 osteoblast cells by activating the bmp2 smad runx2 pathway |
| topic | sword bean pods MC3T3-E1 cells osteoblast BMP2/SMAD/RUNX2 pathway |
| url | https://www.mdpi.com/2072-6643/15/20/4372 |
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