Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers
Abstract Knowledge about population genetic data is important for effective conservation management. Genetic research traditionally requires sampling directly from the organism, for example tissue, which can be challenging, time‐consuming, and harmful to the animal. Environmental DNA (eDNA) approach...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-02-01
|
| Series: | Ecology and Evolution |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ece3.9785 |
| _version_ | 1797894215586283520 |
|---|---|
| author | Silje Halvorsen Lars Korslund Morten Mattingsdal Audun Slettan |
| author_facet | Silje Halvorsen Lars Korslund Morten Mattingsdal Audun Slettan |
| author_sort | Silje Halvorsen |
| collection | DOAJ |
| description | Abstract Knowledge about population genetic data is important for effective conservation management. Genetic research traditionally requires sampling directly from the organism, for example tissue, which can be challenging, time‐consuming, and harmful to the animal. Environmental DNA (eDNA) approaches offer a way to sample genetic material noninvasively. In attempts to estimate population size of aquatic species using eDNA, researchers have found positive correlations between biomass and eDNA concentrations, but the approach is debated because of variations in the production and degrading of DNA in water. Recently, a more accurate eDNA‐approach has emerged, focusing on the genomic differences between individuals. In this study, we used eDNA from water samples to estimate the number of European eel (Anguilla anguilla) individuals by examining haplotypes in the mitochondrial D‐loop region, both in a closed aquatic environment with 10 eels of known haplotypes and in three rivers. The results revealed that it was possible to find every eel haplotype in the eDNA sample collected from the closed environment. We also found 13 unique haplotypes in the eDNA samples from the three rivers, which probably represent 13 eel individuals. This means that it is possible to obtain genomic information from European eel eDNA in water; however, more research is needed to develop the approach into a possible future tool for population quantification. |
| first_indexed | 2024-04-10T07:05:27Z |
| format | Article |
| id | doaj.art-b9cca09659804219beb112709e29ac80 |
| institution | Directory Open Access Journal |
| issn | 2045-7758 |
| language | English |
| last_indexed | 2024-04-10T07:05:27Z |
| publishDate | 2023-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj.art-b9cca09659804219beb112709e29ac802023-02-27T08:56:40ZengWileyEcology and Evolution2045-77582023-02-01132n/an/a10.1002/ece3.9785Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small riversSilje Halvorsen0Lars Korslund1Morten Mattingsdal2Audun Slettan3Faculty of Engineering and Science University of Agder Kristiansand NorwayFaculty of Engineering and Science University of Agder Kristiansand NorwayFaculty of Engineering and Science University of Agder Kristiansand NorwayFaculty of Engineering and Science University of Agder Kristiansand NorwayAbstract Knowledge about population genetic data is important for effective conservation management. Genetic research traditionally requires sampling directly from the organism, for example tissue, which can be challenging, time‐consuming, and harmful to the animal. Environmental DNA (eDNA) approaches offer a way to sample genetic material noninvasively. In attempts to estimate population size of aquatic species using eDNA, researchers have found positive correlations between biomass and eDNA concentrations, but the approach is debated because of variations in the production and degrading of DNA in water. Recently, a more accurate eDNA‐approach has emerged, focusing on the genomic differences between individuals. In this study, we used eDNA from water samples to estimate the number of European eel (Anguilla anguilla) individuals by examining haplotypes in the mitochondrial D‐loop region, both in a closed aquatic environment with 10 eels of known haplotypes and in three rivers. The results revealed that it was possible to find every eel haplotype in the eDNA sample collected from the closed environment. We also found 13 unique haplotypes in the eDNA samples from the three rivers, which probably represent 13 eel individuals. This means that it is possible to obtain genomic information from European eel eDNA in water; however, more research is needed to develop the approach into a possible future tool for population quantification.https://doi.org/10.1002/ece3.9785Anguilla anguillaconservationenvironmental DNAhaplotype countpopulation quantification |
| spellingShingle | Silje Halvorsen Lars Korslund Morten Mattingsdal Audun Slettan Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers Ecology and Evolution Anguilla anguilla conservation environmental DNA haplotype count population quantification |
| title | Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
| title_full | Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
| title_fullStr | Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
| title_full_unstemmed | Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
| title_short | Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
| title_sort | estimating number of european eel anguilla anguilla individuals using environmental dna and haplotype count in small rivers |
| topic | Anguilla anguilla conservation environmental DNA haplotype count population quantification |
| url | https://doi.org/10.1002/ece3.9785 |
| work_keys_str_mv | AT siljehalvorsen estimatingnumberofeuropeaneelanguillaanguillaindividualsusingenvironmentaldnaandhaplotypecountinsmallrivers AT larskorslund estimatingnumberofeuropeaneelanguillaanguillaindividualsusingenvironmentaldnaandhaplotypecountinsmallrivers AT mortenmattingsdal estimatingnumberofeuropeaneelanguillaanguillaindividualsusingenvironmentaldnaandhaplotypecountinsmallrivers AT audunslettan estimatingnumberofeuropeaneelanguillaanguillaindividualsusingenvironmentaldnaandhaplotypecountinsmallrivers |