Analysis of the Physico-Chemical, Mechanical and Biological Properties of Crosslinked Type-I Collagen from Horse Tendon: Towards the Development of Ideal Scaffolding Material for Urethral Regeneration
Urethral stenosis is a pathological condition that consists in the narrowing of the urethral lumen because of the formation of scar tissue. Unfortunately, none of the current surgical approaches represent an optimal solution because of the high stricture recurrence rate. In this context, we prelimin...
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2021-12-01
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| author | Nunzia Gallo Maria Lucia Natali Claudia Curci Angela Picerno Anna Gallone Marco Vulpi Antonio Vitarelli Pasquale Ditonno Mariafrancesca Cascione Fabio Sallustio Rosaria Rinaldi Alessandro Sannino Luca Salvatore |
| author_facet | Nunzia Gallo Maria Lucia Natali Claudia Curci Angela Picerno Anna Gallone Marco Vulpi Antonio Vitarelli Pasquale Ditonno Mariafrancesca Cascione Fabio Sallustio Rosaria Rinaldi Alessandro Sannino Luca Salvatore |
| author_sort | Nunzia Gallo |
| collection | DOAJ |
| description | Urethral stenosis is a pathological condition that consists in the narrowing of the urethral lumen because of the formation of scar tissue. Unfortunately, none of the current surgical approaches represent an optimal solution because of the high stricture recurrence rate. In this context, we preliminarily explored the potential of an insoluble type-I collagen from horse tendon as scaffolding material for the development of innovative devices for the regeneration of injured urethral tracts. Non-porous collagen-based substrates were produced and optimized, in terms of crosslinking density of the macromolecular structure, to either provide mechanical properties compliant with the urinary tract physiological stress and better sustain tissue regeneration. The effect of the adopted crosslinking strategy on the protein integrity and on the substrate physical–chemical, mechanical and biological properties was investigated in comparison with a decellularized matrix from porcine small intestinal submucosa (SIS patch), an extensively used xenograft licensed for clinical use in urology. The optimized production protocols allowed the preservation of the type I collagen native structure and the realization of a substrate with appealing end-use properties. The biological response, preliminarily investigated by immunofluorescence experiments on human adult renal stem/progenitor cells until 28 days, showed the formation of a stem-cell monolayer within 14 days and the onset of spheroids within 28 days. These results suggested the great potential of the collagen-based material for the development of scaffolds for urethral plate regeneration and for in vitro cellular studies. |
| first_indexed | 2024-03-10T03:40:22Z |
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| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T03:40:22Z |
| publishDate | 2021-12-01 |
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| series | Materials |
| spelling | doaj.art-b9cdf61a564645a7983f23446c2c12702023-11-23T09:20:59ZengMDPI AGMaterials1996-19442021-12-011424764810.3390/ma14247648Analysis of the Physico-Chemical, Mechanical and Biological Properties of Crosslinked Type-I Collagen from Horse Tendon: Towards the Development of Ideal Scaffolding Material for Urethral RegenerationNunzia Gallo0Maria Lucia Natali1Claudia Curci2Angela Picerno3Anna Gallone4Marco Vulpi5Antonio Vitarelli6Pasquale Ditonno7Mariafrancesca Cascione8Fabio Sallustio9Rosaria Rinaldi10Alessandro Sannino11Luca Salvatore12Department of Engineering for Innovation, University of Salento, 73100 Lecce, ItalyDepartment of Engineering for Innovation, University of Salento, 73100 Lecce, ItalyDepartment of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari “Aldo Moro”, 70124 Bari, ItalyNephrology Unit, Department of Emergency and Organ Transplantation, University of Bari “Aldo Moro”, 70124 Bari, ItalyDepartment of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari “Aldo Moro”, 70124 Bari, ItalyUrology and Andrology Unit, Department of Emergency and Organ Transplant, University of Bari “Aldo Moro”, 70124 Bari, ItalyUrology and Andrology Unit, Department of Emergency and Organ Transplant, University of Bari “Aldo Moro”, 70124 Bari, ItalyUrology and Andrology Unit, Department of Emergency and Organ Transplant, University of Bari “Aldo Moro”, 70124 Bari, ItalyDepartment of Mathematics and Physics “Ennio De Giorgi”, University of Salento, 73100 Lecce, ItalyDepartment of Interdisciplinary Medicine, University of Bari “Aldo Moro”, 70124 Bari, ItalyDepartment of Mathematics and Physics “Ennio De Giorgi”, University of Salento, 73100 Lecce, ItalyDepartment of Engineering for Innovation, University of Salento, 73100 Lecce, ItalyDepartment of Engineering for Innovation, University of Salento, 73100 Lecce, ItalyUrethral stenosis is a pathological condition that consists in the narrowing of the urethral lumen because of the formation of scar tissue. Unfortunately, none of the current surgical approaches represent an optimal solution because of the high stricture recurrence rate. In this context, we preliminarily explored the potential of an insoluble type-I collagen from horse tendon as scaffolding material for the development of innovative devices for the regeneration of injured urethral tracts. Non-porous collagen-based substrates were produced and optimized, in terms of crosslinking density of the macromolecular structure, to either provide mechanical properties compliant with the urinary tract physiological stress and better sustain tissue regeneration. The effect of the adopted crosslinking strategy on the protein integrity and on the substrate physical–chemical, mechanical and biological properties was investigated in comparison with a decellularized matrix from porcine small intestinal submucosa (SIS patch), an extensively used xenograft licensed for clinical use in urology. The optimized production protocols allowed the preservation of the type I collagen native structure and the realization of a substrate with appealing end-use properties. The biological response, preliminarily investigated by immunofluorescence experiments on human adult renal stem/progenitor cells until 28 days, showed the formation of a stem-cell monolayer within 14 days and the onset of spheroids within 28 days. These results suggested the great potential of the collagen-based material for the development of scaffolds for urethral plate regeneration and for in vitro cellular studies.https://www.mdpi.com/1996-1944/14/24/7648type I collagenurethrastem cellscaffoldtissue engineering |
| spellingShingle | Nunzia Gallo Maria Lucia Natali Claudia Curci Angela Picerno Anna Gallone Marco Vulpi Antonio Vitarelli Pasquale Ditonno Mariafrancesca Cascione Fabio Sallustio Rosaria Rinaldi Alessandro Sannino Luca Salvatore Analysis of the Physico-Chemical, Mechanical and Biological Properties of Crosslinked Type-I Collagen from Horse Tendon: Towards the Development of Ideal Scaffolding Material for Urethral Regeneration Materials type I collagen urethra stem cell scaffold tissue engineering |
| title | Analysis of the Physico-Chemical, Mechanical and Biological Properties of Crosslinked Type-I Collagen from Horse Tendon: Towards the Development of Ideal Scaffolding Material for Urethral Regeneration |
| title_full | Analysis of the Physico-Chemical, Mechanical and Biological Properties of Crosslinked Type-I Collagen from Horse Tendon: Towards the Development of Ideal Scaffolding Material for Urethral Regeneration |
| title_fullStr | Analysis of the Physico-Chemical, Mechanical and Biological Properties of Crosslinked Type-I Collagen from Horse Tendon: Towards the Development of Ideal Scaffolding Material for Urethral Regeneration |
| title_full_unstemmed | Analysis of the Physico-Chemical, Mechanical and Biological Properties of Crosslinked Type-I Collagen from Horse Tendon: Towards the Development of Ideal Scaffolding Material for Urethral Regeneration |
| title_short | Analysis of the Physico-Chemical, Mechanical and Biological Properties of Crosslinked Type-I Collagen from Horse Tendon: Towards the Development of Ideal Scaffolding Material for Urethral Regeneration |
| title_sort | analysis of the physico chemical mechanical and biological properties of crosslinked type i collagen from horse tendon towards the development of ideal scaffolding material for urethral regeneration |
| topic | type I collagen urethra stem cell scaffold tissue engineering |
| url | https://www.mdpi.com/1996-1944/14/24/7648 |
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