Genetic fingerprint and diversity evaluation of halophilic Bacillus species by RAPD-PCR
Abstract Random amplified polymorphic DNA-PCR (RAPD-PCR) is a technique successfully used to generate characteristic fingerprints of different bacteria. Bacillus is a genus that includes heterogeneous species, thus a combination of different techniques is essential for their identification. Here we...
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
2021-08-01
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| Series: | Anais da Academia Brasileira de Ciências |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000600901&tlng=en |
| _version_ | 1798015338407788544 |
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| author | INGRID GEORGINA ORCE FABIANA LILIAN MARTÍNEZ MÓNICA APARICIO MARÍA JULIA TORRES VERÓNICA BEATRIZ RAJAL VERÓNICA PATRICIA IRAZUSTA |
| author_facet | INGRID GEORGINA ORCE FABIANA LILIAN MARTÍNEZ MÓNICA APARICIO MARÍA JULIA TORRES VERÓNICA BEATRIZ RAJAL VERÓNICA PATRICIA IRAZUSTA |
| author_sort | INGRID GEORGINA ORCE |
| collection | DOAJ |
| description | Abstract Random amplified polymorphic DNA-PCR (RAPD-PCR) is a technique successfully used to generate characteristic fingerprints of different bacteria. Bacillus is a genus that includes heterogeneous species, thus a combination of different techniques is essential for their identification. Here we used RAPD-PCR methodology to distinguish among genetically similar strains and to evaluate the genetic diversity of Bacillus species from the Salar del Hombre Muerto, in the Northwest of Argentina. The RAPD-PCR used allowed obtaining different amplification profiles for each Bacillus species and strains. By comparing the fingerprint profiles, we could observe that some of the salt flat isolates showed similar profiles than identified strains. As expected, the bacilli group isolated revealed a wide heterogeneity. RAPD-PCR was found to be a quick and reliable technique to evaluate the diversity of Bacillus strain and was successfully applied to characterize the genetic diversity present in the Salar del Hombre Muerto. |
| first_indexed | 2024-04-11T15:31:39Z |
| format | Article |
| id | doaj.art-b9ceefcca15a4042a74f995d36804686 |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-04-11T15:31:39Z |
| publishDate | 2021-08-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-b9ceefcca15a4042a74f995d368046862022-12-22T04:16:07ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902021-08-0193suppl 310.1590/0001-3765202120191430Genetic fingerprint and diversity evaluation of halophilic Bacillus species by RAPD-PCRINGRID GEORGINA ORCEhttps://orcid.org/0000-0001-7828-0135FABIANA LILIAN MARTÍNEZhttps://orcid.org/0000-0002-6169-0485MÓNICA APARICIOhttps://orcid.org/0000-0003-4220-2981MARÍA JULIA TORREShttps://orcid.org/0000-0002-0991-3813VERÓNICA BEATRIZ RAJALhttps://orcid.org/0000-0002-2290-8920VERÓNICA PATRICIA IRAZUSTAhttps://orcid.org/0000-0001-7436-8543Abstract Random amplified polymorphic DNA-PCR (RAPD-PCR) is a technique successfully used to generate characteristic fingerprints of different bacteria. Bacillus is a genus that includes heterogeneous species, thus a combination of different techniques is essential for their identification. Here we used RAPD-PCR methodology to distinguish among genetically similar strains and to evaluate the genetic diversity of Bacillus species from the Salar del Hombre Muerto, in the Northwest of Argentina. The RAPD-PCR used allowed obtaining different amplification profiles for each Bacillus species and strains. By comparing the fingerprint profiles, we could observe that some of the salt flat isolates showed similar profiles than identified strains. As expected, the bacilli group isolated revealed a wide heterogeneity. RAPD-PCR was found to be a quick and reliable technique to evaluate the diversity of Bacillus strain and was successfully applied to characterize the genetic diversity present in the Salar del Hombre Muerto.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000600901&tlng=enRandom Amplified Polymorphism DNA-PCRHalophilesmolecular characterizationbiodiversitypolymorphic Bacillusgenetic characterization |
| spellingShingle | INGRID GEORGINA ORCE FABIANA LILIAN MARTÍNEZ MÓNICA APARICIO MARÍA JULIA TORRES VERÓNICA BEATRIZ RAJAL VERÓNICA PATRICIA IRAZUSTA Genetic fingerprint and diversity evaluation of halophilic Bacillus species by RAPD-PCR Anais da Academia Brasileira de Ciências Random Amplified Polymorphism DNA-PCR Halophiles molecular characterization biodiversity polymorphic Bacillus genetic characterization |
| title | Genetic fingerprint and diversity evaluation of halophilic Bacillus species by RAPD-PCR |
| title_full | Genetic fingerprint and diversity evaluation of halophilic Bacillus species by RAPD-PCR |
| title_fullStr | Genetic fingerprint and diversity evaluation of halophilic Bacillus species by RAPD-PCR |
| title_full_unstemmed | Genetic fingerprint and diversity evaluation of halophilic Bacillus species by RAPD-PCR |
| title_short | Genetic fingerprint and diversity evaluation of halophilic Bacillus species by RAPD-PCR |
| title_sort | genetic fingerprint and diversity evaluation of halophilic bacillus species by rapd pcr |
| topic | Random Amplified Polymorphism DNA-PCR Halophiles molecular characterization biodiversity polymorphic Bacillus genetic characterization |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000600901&tlng=en |
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