KISS-1 knockdown inhibits cell growth, migration, and invasion in HTR-8/SVneo cells by regulating the GRP54-mediated PI3K/AKT signaling pathway
AbstractRecurrent spontaneous abortions (RSA) affect reproductive health and increase the risk of subsequent abortions. To investigate the role of KISS-1/GPR-54 signaling in RSA progression. Villus tissue was collected from RSA patients, and human trophoblastic HTR-8/SVneo cells were used. KISS-1 an...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Autoimmunity |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/08916934.2023.2297564 |
| _version_ | 1797372882978865152 |
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| author | Lingna Chen Yuying Ruan Liping Ni Guiting Wang Yajuan Gao Jindi Zhang Dingheng Li Haiou Xu |
| author_facet | Lingna Chen Yuying Ruan Liping Ni Guiting Wang Yajuan Gao Jindi Zhang Dingheng Li Haiou Xu |
| author_sort | Lingna Chen |
| collection | DOAJ |
| description | AbstractRecurrent spontaneous abortions (RSA) affect reproductive health and increase the risk of subsequent abortions. To investigate the role of KISS-1/GPR-54 signaling in RSA progression. Villus tissue was collected from RSA patients, and human trophoblastic HTR-8/SVneo cells were used. KISS-1 and GRP54 levels were detected using RT-qPCR and immunohistochemistry. Western blotting was performed to analyze ZO-1 and ZEB1 levels. Cell proliferation was determined via CCK-8 and cell clone formation assays. Transwell assays were performed to assess cell migration and invasion abilities. KISS-1 was down-regulated in the villus tissues of RSA patients. KISS-1 overexpression dramatically inhibited trophoblast proliferation, migration, and invasion. Mechanistically, ZEB1 expression was down-regulated, whereas ZO-1 expression was up-regulated, after KISS-1 overexpression. GPR54 silencing neutralized the effect of KISS-1 in HTR-8/SVneo cells. Additionally, KISS-1 overexpression inactivated the PI3K/AKT signaling pathway through GRP54. The KISS-1/GPR-54 signaling axis regulates RSA progression by regulating the PI3K/AKT signaling pathway. |
| first_indexed | 2024-03-08T18:42:17Z |
| format | Article |
| id | doaj.art-b9d2112cf4ef4b009c13595df4424a86 |
| institution | Directory Open Access Journal |
| issn | 0891-6934 1607-842X |
| language | English |
| last_indexed | 2024-03-08T18:42:17Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Autoimmunity |
| spelling | doaj.art-b9d2112cf4ef4b009c13595df4424a862023-12-29T06:13:14ZengTaylor & Francis GroupAutoimmunity0891-69341607-842X2024-12-0157110.1080/08916934.2023.2297564KISS-1 knockdown inhibits cell growth, migration, and invasion in HTR-8/SVneo cells by regulating the GRP54-mediated PI3K/AKT signaling pathwayLingna Chen0Yuying Ruan1Liping Ni2Guiting Wang3Yajuan Gao4Jindi Zhang5Dingheng Li6Haiou Xu7Hangzhou Women’s Hospital, Hangzhou, ChinaHangzhou Women’s Hospital, Hangzhou, ChinaHangzhou Women’s Hospital, Hangzhou, ChinaHangzhou Women’s Hospital, Hangzhou, ChinaHangzhou Women’s Hospital, Hangzhou, ChinaHangzhou Women’s Hospital, Hangzhou, ChinaHangzhou Women’s Hospital, Hangzhou, ChinaHangzhou Women’s Hospital, Hangzhou, ChinaAbstractRecurrent spontaneous abortions (RSA) affect reproductive health and increase the risk of subsequent abortions. To investigate the role of KISS-1/GPR-54 signaling in RSA progression. Villus tissue was collected from RSA patients, and human trophoblastic HTR-8/SVneo cells were used. KISS-1 and GRP54 levels were detected using RT-qPCR and immunohistochemistry. Western blotting was performed to analyze ZO-1 and ZEB1 levels. Cell proliferation was determined via CCK-8 and cell clone formation assays. Transwell assays were performed to assess cell migration and invasion abilities. KISS-1 was down-regulated in the villus tissues of RSA patients. KISS-1 overexpression dramatically inhibited trophoblast proliferation, migration, and invasion. Mechanistically, ZEB1 expression was down-regulated, whereas ZO-1 expression was up-regulated, after KISS-1 overexpression. GPR54 silencing neutralized the effect of KISS-1 in HTR-8/SVneo cells. Additionally, KISS-1 overexpression inactivated the PI3K/AKT signaling pathway through GRP54. The KISS-1/GPR-54 signaling axis regulates RSA progression by regulating the PI3K/AKT signaling pathway.https://www.tandfonline.com/doi/10.1080/08916934.2023.2297564Recurrent spontaneous abortionKISS-1GPR54PI3K/AKT |
| spellingShingle | Lingna Chen Yuying Ruan Liping Ni Guiting Wang Yajuan Gao Jindi Zhang Dingheng Li Haiou Xu KISS-1 knockdown inhibits cell growth, migration, and invasion in HTR-8/SVneo cells by regulating the GRP54-mediated PI3K/AKT signaling pathway Autoimmunity Recurrent spontaneous abortion KISS-1 GPR54 PI3K/AKT |
| title | KISS-1 knockdown inhibits cell growth, migration, and invasion in HTR-8/SVneo cells by regulating the GRP54-mediated PI3K/AKT signaling pathway |
| title_full | KISS-1 knockdown inhibits cell growth, migration, and invasion in HTR-8/SVneo cells by regulating the GRP54-mediated PI3K/AKT signaling pathway |
| title_fullStr | KISS-1 knockdown inhibits cell growth, migration, and invasion in HTR-8/SVneo cells by regulating the GRP54-mediated PI3K/AKT signaling pathway |
| title_full_unstemmed | KISS-1 knockdown inhibits cell growth, migration, and invasion in HTR-8/SVneo cells by regulating the GRP54-mediated PI3K/AKT signaling pathway |
| title_short | KISS-1 knockdown inhibits cell growth, migration, and invasion in HTR-8/SVneo cells by regulating the GRP54-mediated PI3K/AKT signaling pathway |
| title_sort | kiss 1 knockdown inhibits cell growth migration and invasion in htr 8 svneo cells by regulating the grp54 mediated pi3k akt signaling pathway |
| topic | Recurrent spontaneous abortion KISS-1 GPR54 PI3K/AKT |
| url | https://www.tandfonline.com/doi/10.1080/08916934.2023.2297564 |
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