Ganoderic Acid Me Inhibits Tumor Invasion Through Down-Regulating Matrix Metalloproteinases 2/9 Gene Expression
The effect of ganoderic acid Me (GA-Me), which was purified from the fermentation mycelia of the traditional Chinese medicinal mushroom Ganoderma lucidum as reported (Tang W, Gu TY, Zhong JJ. Biochem Eng J. 2006;32:205–210), on anti-invasion was investigated. Wound healing assay indicated that GA-Me...
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| Format: | Article |
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Elsevier
2008-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S134786131931357X |
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| author | Nian-Hong Chen Jian-Wen Liu Jian-Jiang Zhong |
| author_facet | Nian-Hong Chen Jian-Wen Liu Jian-Jiang Zhong |
| author_sort | Nian-Hong Chen |
| collection | DOAJ |
| description | The effect of ganoderic acid Me (GA-Me), which was purified from the fermentation mycelia of the traditional Chinese medicinal mushroom Ganoderma lucidum as reported (Tang W, Gu TY, Zhong JJ. Biochem Eng J. 2006;32:205–210), on anti-invasion was investigated. Wound healing assay indicated that GA-Me inhibited cell migration of 95-D, a human highly metastatic lung tumor cell line, in dose- and time-dependent manners. Results of cell aggregation and adhesion assays showed that GA-Me promoted cell homotypic aggregation and inhibited cell adherence to extracellular matrix (ECM). In addition, GA-Me suppressed matrix metalloproteinases 2/9 (MMP2/9) gene expressions at both mRNA and protein levels in 95-D cells according to qRT-PCR and Western blotting, respectively. The results demonstrated that GA-Me effectively inhibited tumor invasion, and it might act as a new MMP2/9 inhibitor for antimetastasis treatment of carcinoma cells. Keywords:: ganoderic acid Me (GA-Me), invasion, matrix metalloproteinases 2/9 (MMP2/9) |
| first_indexed | 2024-12-11T23:00:05Z |
| format | Article |
| id | doaj.art-b9d409fa60f94dbf8c132f79267b6dc9 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-11T23:00:05Z |
| publishDate | 2008-01-01 |
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| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-b9d409fa60f94dbf8c132f79267b6dc92022-12-22T00:47:06ZengElsevierJournal of Pharmacological Sciences1347-86132008-01-011082212216Ganoderic Acid Me Inhibits Tumor Invasion Through Down-Regulating Matrix Metalloproteinases 2/9 Gene ExpressionNian-Hong Chen0Jian-Wen Liu1Jian-Jiang Zhong2State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, ChinaKey Laboratory of Microbial Metabolism, Ministry of Education, College of Life Science & Biotechnology, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai 200240, China; Corresponding author. jjzhong@sjtu.edu.cnThe effect of ganoderic acid Me (GA-Me), which was purified from the fermentation mycelia of the traditional Chinese medicinal mushroom Ganoderma lucidum as reported (Tang W, Gu TY, Zhong JJ. Biochem Eng J. 2006;32:205–210), on anti-invasion was investigated. Wound healing assay indicated that GA-Me inhibited cell migration of 95-D, a human highly metastatic lung tumor cell line, in dose- and time-dependent manners. Results of cell aggregation and adhesion assays showed that GA-Me promoted cell homotypic aggregation and inhibited cell adherence to extracellular matrix (ECM). In addition, GA-Me suppressed matrix metalloproteinases 2/9 (MMP2/9) gene expressions at both mRNA and protein levels in 95-D cells according to qRT-PCR and Western blotting, respectively. The results demonstrated that GA-Me effectively inhibited tumor invasion, and it might act as a new MMP2/9 inhibitor for antimetastasis treatment of carcinoma cells. Keywords:: ganoderic acid Me (GA-Me), invasion, matrix metalloproteinases 2/9 (MMP2/9)http://www.sciencedirect.com/science/article/pii/S134786131931357X |
| spellingShingle | Nian-Hong Chen Jian-Wen Liu Jian-Jiang Zhong Ganoderic Acid Me Inhibits Tumor Invasion Through Down-Regulating Matrix Metalloproteinases 2/9 Gene Expression Journal of Pharmacological Sciences |
| title | Ganoderic Acid Me Inhibits Tumor Invasion Through Down-Regulating Matrix Metalloproteinases 2/9 Gene Expression |
| title_full | Ganoderic Acid Me Inhibits Tumor Invasion Through Down-Regulating Matrix Metalloproteinases 2/9 Gene Expression |
| title_fullStr | Ganoderic Acid Me Inhibits Tumor Invasion Through Down-Regulating Matrix Metalloproteinases 2/9 Gene Expression |
| title_full_unstemmed | Ganoderic Acid Me Inhibits Tumor Invasion Through Down-Regulating Matrix Metalloproteinases 2/9 Gene Expression |
| title_short | Ganoderic Acid Me Inhibits Tumor Invasion Through Down-Regulating Matrix Metalloproteinases 2/9 Gene Expression |
| title_sort | ganoderic acid me inhibits tumor invasion through down regulating matrix metalloproteinases 2 9 gene expression |
| url | http://www.sciencedirect.com/science/article/pii/S134786131931357X |
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