Radiation monitoring with plant-based biotas and an automated micronucleus scoring approach
In this work, we investigated the response of the stem cells from the roots of Allium Cepa (meristems) to ionizing and non-ionizing radiations of different qualities. Allium cepa (Onion) is a well-established in-vivo standard model, widely used in cytogenetic studies for different environmental poll...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2023-01-01
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| Series: | EPJ Web of Conferences |
| Subjects: | |
| Online Access: | https://www.epj-conferences.org/articles/epjconf/pdf/2023/14/epjconf_animma2023_09007.pdf |
| _version_ | 1797344041738698752 |
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| author | d’Errico F. Barco F. Butini T. Cascone M.G. Ciolini R. Quattrocchi M. Rosellini E. Hulber T. Torres Novaes J.A. Xavier M.N. de Souza Lalic S. |
| author_facet | d’Errico F. Barco F. Butini T. Cascone M.G. Ciolini R. Quattrocchi M. Rosellini E. Hulber T. Torres Novaes J.A. Xavier M.N. de Souza Lalic S. |
| author_sort | d’Errico F. |
| collection | DOAJ |
| description | In this work, we investigated the response of the stem cells from the roots of Allium Cepa (meristems) to ionizing and non-ionizing radiations of different qualities. Allium cepa (Onion) is a well-established in-vivo standard model, widely used in cytogenetic studies for different environmental pollutants. Endpoints, such as chromosomal aberrations (CAs), micronuclei (MNs), and disturbance in the mitotic cycle of root meristematic cells of onion are frequently used to determine the cytotoxic and genotoxic potential of different environmental pollutants. Traditionally, these studies have been carried out to assess chemical toxicity, while the toxicity of ionizing radiation has been studied less extensively. We also examined the repurposing of a previously described optical microscope system originally designed for automated non-fluorescent micronucleus (MN) scoring in binucleated peripheral lymphocytes. The microscope system relies on hardware and software layers in parallel in order to optimize the performance in automated MN scoring. |
| first_indexed | 2024-03-08T10:56:32Z |
| format | Article |
| id | doaj.art-b9d5cbf712234971b4188adaa2a4662b |
| institution | Directory Open Access Journal |
| issn | 2100-014X |
| language | English |
| last_indexed | 2024-03-08T10:56:32Z |
| publishDate | 2023-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | EPJ Web of Conferences |
| spelling | doaj.art-b9d5cbf712234971b4188adaa2a4662b2024-01-26T16:33:48ZengEDP SciencesEPJ Web of Conferences2100-014X2023-01-012880900710.1051/epjconf/202328809007epjconf_animma2023_09007Radiation monitoring with plant-based biotas and an automated micronucleus scoring approachd’Errico F.0Barco F.1Butini T.2Cascone M.G.3Ciolini R.4Quattrocchi M.5Rosellini E.6Hulber T.7Torres Novaes J.A.8Xavier M.N.9de Souza Lalic S.10School of Engineering, University ofSchool of Engineering, University ofSchool of Engineering, University ofSchool of Engineering, University ofSchool of Engineering, University ofDepartment of RadiologySchool of Engineering, University ofRadosysDepartment of Physics, Federal University ofDepartment of Physics, Federal University ofDepartment of Physics, Federal University ofIn this work, we investigated the response of the stem cells from the roots of Allium Cepa (meristems) to ionizing and non-ionizing radiations of different qualities. Allium cepa (Onion) is a well-established in-vivo standard model, widely used in cytogenetic studies for different environmental pollutants. Endpoints, such as chromosomal aberrations (CAs), micronuclei (MNs), and disturbance in the mitotic cycle of root meristematic cells of onion are frequently used to determine the cytotoxic and genotoxic potential of different environmental pollutants. Traditionally, these studies have been carried out to assess chemical toxicity, while the toxicity of ionizing radiation has been studied less extensively. We also examined the repurposing of a previously described optical microscope system originally designed for automated non-fluorescent micronucleus (MN) scoring in binucleated peripheral lymphocytes. The microscope system relies on hardware and software layers in parallel in order to optimize the performance in automated MN scoring.https://www.epj-conferences.org/articles/epjconf/pdf/2023/14/epjconf_animma2023_09007.pdfbiotamicronucleusionizing radiationnonionizing radiation |
| spellingShingle | d’Errico F. Barco F. Butini T. Cascone M.G. Ciolini R. Quattrocchi M. Rosellini E. Hulber T. Torres Novaes J.A. Xavier M.N. de Souza Lalic S. Radiation monitoring with plant-based biotas and an automated micronucleus scoring approach EPJ Web of Conferences biota micronucleus ionizing radiation nonionizing radiation |
| title | Radiation monitoring with plant-based biotas and an automated micronucleus scoring approach |
| title_full | Radiation monitoring with plant-based biotas and an automated micronucleus scoring approach |
| title_fullStr | Radiation monitoring with plant-based biotas and an automated micronucleus scoring approach |
| title_full_unstemmed | Radiation monitoring with plant-based biotas and an automated micronucleus scoring approach |
| title_short | Radiation monitoring with plant-based biotas and an automated micronucleus scoring approach |
| title_sort | radiation monitoring with plant based biotas and an automated micronucleus scoring approach |
| topic | biota micronucleus ionizing radiation nonionizing radiation |
| url | https://www.epj-conferences.org/articles/epjconf/pdf/2023/14/epjconf_animma2023_09007.pdf |
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