Glucose oxidase converted into a general sugar-oxidase
Abstract Entrapment of glucose oxidase (GOx) within metallic gold converts this widely used enzyme into a general saccharide oxidase. The following sugar molecules were oxidized by the entrapped enzyme (in addition to d-glucose): fructose, xylose, l-glucose, glucose-6-phosphate, sucrose, lactose, me...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-14957-6 |
| _version_ | 1818257071237234688 |
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| author | Yael Baruch-Shpigler David Avnir |
| author_facet | Yael Baruch-Shpigler David Avnir |
| author_sort | Yael Baruch-Shpigler |
| collection | DOAJ |
| description | Abstract Entrapment of glucose oxidase (GOx) within metallic gold converts this widely used enzyme into a general saccharide oxidase. The following sugar molecules were oxidized by the entrapped enzyme (in addition to d-glucose): fructose, xylose, l-glucose, glucose-6-phosphate, sucrose, lactose, methylglucoside, and the tri-saccharide raffinose. With the exception of raffinose, none of these sugars have a natural specific oxidase. The origin of this generalization of activity is attributed to the strong protein-gold 3D interactions and to the strong interactions of the co-entrapped CTAB with both the gold, and the protein. It is proposed that these interactions induce conformational changes in the channel leading to the active site, which is located at the interface between the two units of the dimeric GOx protein. The observations are compatible with affecting the specific conformation change of pulling apart and opening this gate-keeper, rendering the active site accessible to a variety of substrates. The entrapment methodology was also found to increase the thermal stability of GOx up to 100 °C and to allow its convenient reuse, two features of practical importance. |
| first_indexed | 2024-12-12T17:37:49Z |
| format | Article |
| id | doaj.art-b9d8bd3ce9194c0da974f03593368fdf |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T17:37:49Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b9d8bd3ce9194c0da974f03593368fdf2022-12-22T00:17:11ZengNature PortfolioScientific Reports2045-23222022-06-0112111010.1038/s41598-022-14957-6Glucose oxidase converted into a general sugar-oxidaseYael Baruch-Shpigler0David Avnir1Institute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of JerusalemInstitute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of JerusalemAbstract Entrapment of glucose oxidase (GOx) within metallic gold converts this widely used enzyme into a general saccharide oxidase. The following sugar molecules were oxidized by the entrapped enzyme (in addition to d-glucose): fructose, xylose, l-glucose, glucose-6-phosphate, sucrose, lactose, methylglucoside, and the tri-saccharide raffinose. With the exception of raffinose, none of these sugars have a natural specific oxidase. The origin of this generalization of activity is attributed to the strong protein-gold 3D interactions and to the strong interactions of the co-entrapped CTAB with both the gold, and the protein. It is proposed that these interactions induce conformational changes in the channel leading to the active site, which is located at the interface between the two units of the dimeric GOx protein. The observations are compatible with affecting the specific conformation change of pulling apart and opening this gate-keeper, rendering the active site accessible to a variety of substrates. The entrapment methodology was also found to increase the thermal stability of GOx up to 100 °C and to allow its convenient reuse, two features of practical importance.https://doi.org/10.1038/s41598-022-14957-6 |
| spellingShingle | Yael Baruch-Shpigler David Avnir Glucose oxidase converted into a general sugar-oxidase Scientific Reports |
| title | Glucose oxidase converted into a general sugar-oxidase |
| title_full | Glucose oxidase converted into a general sugar-oxidase |
| title_fullStr | Glucose oxidase converted into a general sugar-oxidase |
| title_full_unstemmed | Glucose oxidase converted into a general sugar-oxidase |
| title_short | Glucose oxidase converted into a general sugar-oxidase |
| title_sort | glucose oxidase converted into a general sugar oxidase |
| url | https://doi.org/10.1038/s41598-022-14957-6 |
| work_keys_str_mv | AT yaelbaruchshpigler glucoseoxidaseconvertedintoageneralsugaroxidase AT davidavnir glucoseoxidaseconvertedintoageneralsugaroxidase |