In-depth analysis of erythrose reductase homologs in Yarrowia lipolytica
Abstract The unconventional yeast Yarrowia lipolytica produces erythritol as an osmoprotectant to adapt to osmotic stress. In this study, the array of putative erythrose reductases, responsible for the conversion of d-erythrose to erythritol, was analyzed. Single knockout and multiple knockout strai...
| Asıl Yazarlar: | , , , |
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| Materyal Türü: | Makale |
| Dil: | English |
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Nature Portfolio
2023-06-01
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| Seri Bilgileri: | Scientific Reports |
| Online Erişim: | https://doi.org/10.1038/s41598-023-36152-x |
| _version_ | 1827928727987683328 |
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| author | Mateusz Szczepańczyk Dorota A. Rzechonek Cécile Neuvéglise Aleksandra M. Mirończuk |
| author_facet | Mateusz Szczepańczyk Dorota A. Rzechonek Cécile Neuvéglise Aleksandra M. Mirończuk |
| author_sort | Mateusz Szczepańczyk |
| collection | DOAJ |
| description | Abstract The unconventional yeast Yarrowia lipolytica produces erythritol as an osmoprotectant to adapt to osmotic stress. In this study, the array of putative erythrose reductases, responsible for the conversion of d-erythrose to erythritol, was analyzed. Single knockout and multiple knockout strains were tested for their ability to produce polyols in osmotic stress conditions. Lack of six of the reductase genes does not affect erythritol significantly, as the production of this polyol is comparable to the control strain. Deletion of eight of the homologous erythrose reductase genes resulted in a 91% decrease in erythritol synthesis, a 53% increase in mannitol synthesis, and an almost 8-fold increase in arabitol synthesis as compared to the control strain. Additionally, the utilization of glycerol was impaired in the media with induced higher osmotic pressure. The results of this research may shed new light on the production of arabitol and mannitol from glycerol by Y. lipolytica and help to develop strategies for further modification in polyol pathways in these microorganisms. |
| first_indexed | 2024-03-13T06:12:52Z |
| format | Article |
| id | doaj.art-b9d8d5126cdd42a4b51f182fe34a2d7c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T06:12:52Z |
| publishDate | 2023-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b9d8d5126cdd42a4b51f182fe34a2d7c2023-06-11T11:11:32ZengNature PortfolioScientific Reports2045-23222023-06-0113111510.1038/s41598-023-36152-xIn-depth analysis of erythrose reductase homologs in Yarrowia lipolyticaMateusz Szczepańczyk0Dorota A. Rzechonek1Cécile Neuvéglise2Aleksandra M. Mirończuk3Laboratory for Biosustainability, Institute of Environmental Biology, Wrocław University of Environmental and Life SciencesLaboratory for Biosustainability, Institute of Environmental Biology, Wrocław University of Environmental and Life SciencesINRAE, Institut Agro, SPO, University MontpellierLaboratory for Biosustainability, Institute of Environmental Biology, Wrocław University of Environmental and Life SciencesAbstract The unconventional yeast Yarrowia lipolytica produces erythritol as an osmoprotectant to adapt to osmotic stress. In this study, the array of putative erythrose reductases, responsible for the conversion of d-erythrose to erythritol, was analyzed. Single knockout and multiple knockout strains were tested for their ability to produce polyols in osmotic stress conditions. Lack of six of the reductase genes does not affect erythritol significantly, as the production of this polyol is comparable to the control strain. Deletion of eight of the homologous erythrose reductase genes resulted in a 91% decrease in erythritol synthesis, a 53% increase in mannitol synthesis, and an almost 8-fold increase in arabitol synthesis as compared to the control strain. Additionally, the utilization of glycerol was impaired in the media with induced higher osmotic pressure. The results of this research may shed new light on the production of arabitol and mannitol from glycerol by Y. lipolytica and help to develop strategies for further modification in polyol pathways in these microorganisms.https://doi.org/10.1038/s41598-023-36152-x |
| spellingShingle | Mateusz Szczepańczyk Dorota A. Rzechonek Cécile Neuvéglise Aleksandra M. Mirończuk In-depth analysis of erythrose reductase homologs in Yarrowia lipolytica Scientific Reports |
| title | In-depth analysis of erythrose reductase homologs in Yarrowia lipolytica |
| title_full | In-depth analysis of erythrose reductase homologs in Yarrowia lipolytica |
| title_fullStr | In-depth analysis of erythrose reductase homologs in Yarrowia lipolytica |
| title_full_unstemmed | In-depth analysis of erythrose reductase homologs in Yarrowia lipolytica |
| title_short | In-depth analysis of erythrose reductase homologs in Yarrowia lipolytica |
| title_sort | in depth analysis of erythrose reductase homologs in yarrowia lipolytica |
| url | https://doi.org/10.1038/s41598-023-36152-x |
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