Single-cell chromatin accessibility and transcriptomic characterization of Behcet’s disease
Abstract Behect’s disease is a chronic vasculitis characterized by complex multi-organ immune aberrations. However, a comprehensive understanding of the gene-regulatory profile of peripheral autoimmunity and the diverse immune responses across distinct cell types in Behcet’s disease (BD) is still la...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-10-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-023-05420-x |
| _version_ | 1797557234086969344 |
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| author | Wen Shi Jinguo Ye Zhuoxing Shi Caineng Pan Qikai Zhang Yuheng Lin Dan Liang Yizhi Liu Xianchai Lin Yingfeng Zheng |
| author_facet | Wen Shi Jinguo Ye Zhuoxing Shi Caineng Pan Qikai Zhang Yuheng Lin Dan Liang Yizhi Liu Xianchai Lin Yingfeng Zheng |
| author_sort | Wen Shi |
| collection | DOAJ |
| description | Abstract Behect’s disease is a chronic vasculitis characterized by complex multi-organ immune aberrations. However, a comprehensive understanding of the gene-regulatory profile of peripheral autoimmunity and the diverse immune responses across distinct cell types in Behcet’s disease (BD) is still lacking. Here, we present a multi-omic single-cell study of 424,817 cells in BD patients and non-BD individuals. This study maps chromatin accessibility and gene expression in the same biological samples, unraveling vast cellular heterogeneity. We identify widespread cell-type-specific, disease-associated active and pro-inflammatory immunity in both transcript and epigenomic aspects. Notably, integrative multi-omic analysis reveals putative TF regulators that might contribute to chromatin accessibility and gene expression in BD. Moreover, we predicted gene-regulatory networks within nominated TF activators, including AP-1, NF-kB, and ETS transcript factor families, which may regulate cellular interaction and govern inflammation. Our study illustrates the epigenetic and transcriptional landscape in BD peripheral blood and expands understanding of potential epigenomic immunopathology in this disease. |
| first_indexed | 2024-03-10T17:14:27Z |
| format | Article |
| id | doaj.art-b9daa3d7f2ef4f66934e5a5f164975ba |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-03-10T17:14:27Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-b9daa3d7f2ef4f66934e5a5f164975ba2023-11-20T10:33:57ZengNature PortfolioCommunications Biology2399-36422023-10-016111710.1038/s42003-023-05420-xSingle-cell chromatin accessibility and transcriptomic characterization of Behcet’s diseaseWen Shi0Jinguo Ye1Zhuoxing Shi2Caineng Pan3Qikai Zhang4Yuheng Lin5Dan Liang6Yizhi Liu7Xianchai Lin8Yingfeng Zheng9State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceAbstract Behect’s disease is a chronic vasculitis characterized by complex multi-organ immune aberrations. However, a comprehensive understanding of the gene-regulatory profile of peripheral autoimmunity and the diverse immune responses across distinct cell types in Behcet’s disease (BD) is still lacking. Here, we present a multi-omic single-cell study of 424,817 cells in BD patients and non-BD individuals. This study maps chromatin accessibility and gene expression in the same biological samples, unraveling vast cellular heterogeneity. We identify widespread cell-type-specific, disease-associated active and pro-inflammatory immunity in both transcript and epigenomic aspects. Notably, integrative multi-omic analysis reveals putative TF regulators that might contribute to chromatin accessibility and gene expression in BD. Moreover, we predicted gene-regulatory networks within nominated TF activators, including AP-1, NF-kB, and ETS transcript factor families, which may regulate cellular interaction and govern inflammation. Our study illustrates the epigenetic and transcriptional landscape in BD peripheral blood and expands understanding of potential epigenomic immunopathology in this disease.https://doi.org/10.1038/s42003-023-05420-x |
| spellingShingle | Wen Shi Jinguo Ye Zhuoxing Shi Caineng Pan Qikai Zhang Yuheng Lin Dan Liang Yizhi Liu Xianchai Lin Yingfeng Zheng Single-cell chromatin accessibility and transcriptomic characterization of Behcet’s disease Communications Biology |
| title | Single-cell chromatin accessibility and transcriptomic characterization of Behcet’s disease |
| title_full | Single-cell chromatin accessibility and transcriptomic characterization of Behcet’s disease |
| title_fullStr | Single-cell chromatin accessibility and transcriptomic characterization of Behcet’s disease |
| title_full_unstemmed | Single-cell chromatin accessibility and transcriptomic characterization of Behcet’s disease |
| title_short | Single-cell chromatin accessibility and transcriptomic characterization of Behcet’s disease |
| title_sort | single cell chromatin accessibility and transcriptomic characterization of behcet s disease |
| url | https://doi.org/10.1038/s42003-023-05420-x |
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