Cytotoxic Activity of Juniperus excelsa M. Bieb. Leaves Essential Oil in Breast Cancer Cell Lines
<strong>Background and objectives:</strong> <em>Juniperus excelsa</em> is a flowering plant that has been applied as traditional medicine for treatment of various disorders such as dysmenorrhea, bronchitis and colds, jaundice and tuberculosis. The aims of the present study we...
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| Format: | Article |
| Language: | English |
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Iranian Society of Pharmacognosy
2019-04-01
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| Series: | Research Journal of Pharmacognosy |
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| Online Access: | http://www.rjpharmacognosy.ir/article_84313_e18d435932a708c8c05f9be38c2c2ad8.pdf |
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| author | Mahnaz Khanavi Aysheh Enayati Mohammad Reza Shams Ardekani Tahmineh Akbarzadeh Elahe Karimpour Razkenari Mahdieh Eftekhari* |
| author_facet | Mahnaz Khanavi Aysheh Enayati Mohammad Reza Shams Ardekani Tahmineh Akbarzadeh Elahe Karimpour Razkenari Mahdieh Eftekhari* |
| author_sort | Mahnaz Khanavi |
| collection | DOAJ |
| description | <strong>Background and objectives:</strong> <em>Juniperus excelsa</em> is a flowering plant that has been applied as traditional medicine for treatment of various disorders such as dysmenorrhea, bronchitis and colds, jaundice and tuberculosis. The aims of the present study were analyzing <em>J. excelsa</em> essential oil and investigation of its cytotoxic activity on three breast cancer cell lines. <strong>Methods: </strong> <em>Juniperus excelsa</em> leaves were collected from Dena mountains, located in the south-west of Iran. The composition of the essential oil was analyzed by gas chromatography-mass spectrometry (GC/MS). Cytotoxic activity was evaluated using MTT assay. <strong>Results: </strong>Forty-one components, related to 99.83% of the total oil, were identified. Monoterpene hydrocarbons represented the major components of the volatile oil while α-pinene (73.27%) was the major component. The essential oil showed significant cytotoxic activity against breast cancer cell lines MCF-7 (IC<sub>50</sub>=0.084 µg/mL), MDA-MB-231 (IC<sub>50</sub>=0.090 µg/mL) and T-47D (IC<sub>50</sub>=0.124 µg/mL). <strong> Conclusion:</strong> The analysis of <em>J. excelsa </em>oil revealed α-pinene and cedrol as the main compounds of the volatile oil that could justifiy its remarkable cytotoxic effect against the tested cell lines. |
| first_indexed | 2024-12-14T15:12:35Z |
| format | Article |
| id | doaj.art-b9db4285e9a241fbb7aeb9fc194fc8b7 |
| institution | Directory Open Access Journal |
| issn | 2345-4458 2345-5977 |
| language | English |
| last_indexed | 2024-12-14T15:12:35Z |
| publishDate | 2019-04-01 |
| publisher | Iranian Society of Pharmacognosy |
| record_format | Article |
| series | Research Journal of Pharmacognosy |
| spelling | doaj.art-b9db4285e9a241fbb7aeb9fc194fc8b72022-12-21T22:56:29ZengIranian Society of PharmacognosyResearch Journal of Pharmacognosy2345-44582345-59772019-04-01621710.22127/rjp.2019.8431384313Cytotoxic Activity of Juniperus excelsa M. Bieb. Leaves Essential Oil in Breast Cancer Cell LinesMahnaz Khanavi0Aysheh Enayati1Mohammad Reza Shams Ardekani2Tahmineh Akbarzadeh3Elahe Karimpour Razkenari4Mahdieh Eftekhari*5Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Faculty of Land and Food Systems, University of British Columbia, Vancouver, BC, Canada.Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Persian Medicine and Pharmacy Research Center, Tehran University of Medical Sciences, Tehran, Iran.Persian Medicine and Pharmacy Research Center, Tehran University of Medical Sciences, Tehran, Iran.Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.Persian Medicine and Pharmacy Research Center, Tehran University of Medical Sciences, Tehran, Iran.<strong>Background and objectives:</strong> <em>Juniperus excelsa</em> is a flowering plant that has been applied as traditional medicine for treatment of various disorders such as dysmenorrhea, bronchitis and colds, jaundice and tuberculosis. The aims of the present study were analyzing <em>J. excelsa</em> essential oil and investigation of its cytotoxic activity on three breast cancer cell lines. <strong>Methods: </strong> <em>Juniperus excelsa</em> leaves were collected from Dena mountains, located in the south-west of Iran. The composition of the essential oil was analyzed by gas chromatography-mass spectrometry (GC/MS). Cytotoxic activity was evaluated using MTT assay. <strong>Results: </strong>Forty-one components, related to 99.83% of the total oil, were identified. Monoterpene hydrocarbons represented the major components of the volatile oil while α-pinene (73.27%) was the major component. The essential oil showed significant cytotoxic activity against breast cancer cell lines MCF-7 (IC<sub>50</sub>=0.084 µg/mL), MDA-MB-231 (IC<sub>50</sub>=0.090 µg/mL) and T-47D (IC<sub>50</sub>=0.124 µg/mL). <strong> Conclusion:</strong> The analysis of <em>J. excelsa </em>oil revealed α-pinene and cedrol as the main compounds of the volatile oil that could justifiy its remarkable cytotoxic effect against the tested cell lines.http://www.rjpharmacognosy.ir/article_84313_e18d435932a708c8c05f9be38c2c2ad8.pdfCytotoxicJuniperus excelsaMTT assay |
| spellingShingle | Mahnaz Khanavi Aysheh Enayati Mohammad Reza Shams Ardekani Tahmineh Akbarzadeh Elahe Karimpour Razkenari Mahdieh Eftekhari* Cytotoxic Activity of Juniperus excelsa M. Bieb. Leaves Essential Oil in Breast Cancer Cell Lines Research Journal of Pharmacognosy Cytotoxic Juniperus excelsa MTT assay |
| title | Cytotoxic Activity of Juniperus excelsa M. Bieb. Leaves Essential Oil in Breast Cancer Cell Lines |
| title_full | Cytotoxic Activity of Juniperus excelsa M. Bieb. Leaves Essential Oil in Breast Cancer Cell Lines |
| title_fullStr | Cytotoxic Activity of Juniperus excelsa M. Bieb. Leaves Essential Oil in Breast Cancer Cell Lines |
| title_full_unstemmed | Cytotoxic Activity of Juniperus excelsa M. Bieb. Leaves Essential Oil in Breast Cancer Cell Lines |
| title_short | Cytotoxic Activity of Juniperus excelsa M. Bieb. Leaves Essential Oil in Breast Cancer Cell Lines |
| title_sort | cytotoxic activity of juniperus excelsa m bieb leaves essential oil in breast cancer cell lines |
| topic | Cytotoxic Juniperus excelsa MTT assay |
| url | http://www.rjpharmacognosy.ir/article_84313_e18d435932a708c8c05f9be38c2c2ad8.pdf |
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