Cytotoxic and Anti-Inflammatory Activities of Garcinia xanthochymus Extracts on Cell Lines
Background: Garcinia xanthochymus extract has been reported to have several pharmacological properties. This study was conducted to evaluate cytotoxic and anti-inflammatory activities of G. xanthochymus extracts on cell lines. Methods: The roots and stem barks of plant were extracted using maceratio...
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| Format: | Article |
| Language: | English |
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Universitas Indonesia
2016-04-01
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| Series: | Makara Journal of Health Research |
| Subjects: | |
| Online Access: | https://scholarhub.ui.ac.id/mjhr/vol20/iss1/3/ |
| _version_ | 1797847315734593536 |
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| author | Hanisuhana Hamidon Muhammad Taher Juliana M. Jaffri Tg M. Tg Zakaria Wan MAW Sulaiman Deny Susanti Solachuddin JA Ichwan Zainul A. Zakaria |
| author_facet | Hanisuhana Hamidon Muhammad Taher Juliana M. Jaffri Tg M. Tg Zakaria Wan MAW Sulaiman Deny Susanti Solachuddin JA Ichwan Zainul A. Zakaria |
| author_sort | Hanisuhana Hamidon |
| collection | DOAJ |
| description | Background: Garcinia xanthochymus extract has been reported to have several pharmacological properties. This study was conducted to evaluate cytotoxic and anti-inflammatory activities of G. xanthochymus extracts on cell lines. Methods: The roots and stem barks of plant were extracted using maceration method with n-hexane, dichloromethane and methanol, successively. Cytotoxic activity of the extracts was tested against MCF-7 breast adenocarcinoma using MTT assay. Anti-inflammatory study was evaluated using RAW 264.7 mouse macrophage cells. The nitric oxide production in LPS-stimulated cells was measured using Griess reagent. Results: The results of cytotoxic and anti-inflammatory study showed that dichloromethane and n-hexane extracts of root and stem bark exhibited cytotoxic activity in dose-dependent manner. Meanwhile, for anti-inflammatory study, all root extracts together with stem bark dichloromethane and n-hexane extracts reduce NO production in LPS-stimulated cells in dose dependent manner. Conclusions: This finding indicated that G. xanthochymus extracts might become interesting candidate for treatment of cancer and inflammation. |
| first_indexed | 2024-04-09T18:10:29Z |
| format | Article |
| id | doaj.art-b9dcb789481f4fcc99143770dac3a76e |
| institution | Directory Open Access Journal |
| issn | 2356-3664 2356-3656 |
| language | English |
| last_indexed | 2024-04-09T18:10:29Z |
| publishDate | 2016-04-01 |
| publisher | Universitas Indonesia |
| record_format | Article |
| series | Makara Journal of Health Research |
| spelling | doaj.art-b9dcb789481f4fcc99143770dac3a76e2023-04-14T03:01:04ZengUniversitas IndonesiaMakara Journal of Health Research2356-36642356-36562016-04-01201111710.7454/msk.v20i1.55993278Cytotoxic and Anti-Inflammatory Activities of Garcinia xanthochymus Extracts on Cell LinesHanisuhana Hamidon0Muhammad Taher1Juliana M. Jaffri2Tg M. Tg Zakaria3Wan MAW Sulaiman4Deny Susanti5Solachuddin JA Ichwan6Zainul A. Zakaria7Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, MalaysiaDepartment of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, MalaysiaDepartment of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, MalaysiaDepartment of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, MalaysiaDepartment of Basic Medical Science, Faculty of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, MalaysiaDepartment of Chemistry, Faculty of Science, International Islamic University Malaysia, 25200 Kuantan, MalaysiaFaculty of Dentistry, International Islamic University Malaysia, 25200 Kuantan, MalaysiaDepartment of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, MalaysiaBackground: Garcinia xanthochymus extract has been reported to have several pharmacological properties. This study was conducted to evaluate cytotoxic and anti-inflammatory activities of G. xanthochymus extracts on cell lines. Methods: The roots and stem barks of plant were extracted using maceration method with n-hexane, dichloromethane and methanol, successively. Cytotoxic activity of the extracts was tested against MCF-7 breast adenocarcinoma using MTT assay. Anti-inflammatory study was evaluated using RAW 264.7 mouse macrophage cells. The nitric oxide production in LPS-stimulated cells was measured using Griess reagent. Results: The results of cytotoxic and anti-inflammatory study showed that dichloromethane and n-hexane extracts of root and stem bark exhibited cytotoxic activity in dose-dependent manner. Meanwhile, for anti-inflammatory study, all root extracts together with stem bark dichloromethane and n-hexane extracts reduce NO production in LPS-stimulated cells in dose dependent manner. Conclusions: This finding indicated that G. xanthochymus extracts might become interesting candidate for treatment of cancer and inflammation.https://scholarhub.ui.ac.id/mjhr/vol20/iss1/3/garcinia xanthochymusguttiferaecytotoxicityanti-inflammatoryin vitro |
| spellingShingle | Hanisuhana Hamidon Muhammad Taher Juliana M. Jaffri Tg M. Tg Zakaria Wan MAW Sulaiman Deny Susanti Solachuddin JA Ichwan Zainul A. Zakaria Cytotoxic and Anti-Inflammatory Activities of Garcinia xanthochymus Extracts on Cell Lines Makara Journal of Health Research garcinia xanthochymus guttiferae cytotoxicity anti-inflammatory in vitro |
| title | Cytotoxic and Anti-Inflammatory Activities of Garcinia xanthochymus Extracts on Cell Lines |
| title_full | Cytotoxic and Anti-Inflammatory Activities of Garcinia xanthochymus Extracts on Cell Lines |
| title_fullStr | Cytotoxic and Anti-Inflammatory Activities of Garcinia xanthochymus Extracts on Cell Lines |
| title_full_unstemmed | Cytotoxic and Anti-Inflammatory Activities of Garcinia xanthochymus Extracts on Cell Lines |
| title_short | Cytotoxic and Anti-Inflammatory Activities of Garcinia xanthochymus Extracts on Cell Lines |
| title_sort | cytotoxic and anti inflammatory activities of garcinia xanthochymus extracts on cell lines |
| topic | garcinia xanthochymus guttiferae cytotoxicity anti-inflammatory in vitro |
| url | https://scholarhub.ui.ac.id/mjhr/vol20/iss1/3/ |
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