Characterization of NAC transcription factor NtNAC028 as a regulator of leaf senescence and stress responses
NAC proteins constitute one of the largest transcription factor families and are involved in regulation of plant development and stress responses. Our previous transcriptome analyses of tobacco revealed a significant increase in the expression of NtNAC028 during leaf yellowing. In this study, we fou...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-08-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.941026/full |
| _version_ | 1811321434376503296 |
|---|---|
| author | Lichao Wen Tao Liu Zhichao Deng Zenglin Zhang Qi Wang Weifeng Wang Wei Li Yongfeng Guo |
| author_facet | Lichao Wen Tao Liu Zhichao Deng Zenglin Zhang Qi Wang Weifeng Wang Wei Li Yongfeng Guo |
| author_sort | Lichao Wen |
| collection | DOAJ |
| description | NAC proteins constitute one of the largest transcription factor families and are involved in regulation of plant development and stress responses. Our previous transcriptome analyses of tobacco revealed a significant increase in the expression of NtNAC028 during leaf yellowing. In this study, we found that NtNAC028 was rapidly upregulated in response to high salinity, dehydration, and abscisic acid (ABA) stresses, suggesting a vital role of this gene in abiotic stress response. NtNAC028 loss-of-function tobacco plants generated via CRISPR-Cas9 showed delayed leaf senescence and increased tolerance to drought and salt stresses. Meanwhile NtNAC028 overexpression led to precocious leaf senescence and hypersensitivity to abiotic stresses in Arabidopsis, indicating that NtNAC028 functions as a positive regulator of natural leaf senescence and a negative regulator of stress tolerance. Furthermore, NtNAC028-overexpressing Arabidopsis plants showed lower antioxidant enzyme activities, higher reactive oxygen species (ROS), and H2O2 accumulation under high salinity, resulted in more severe oxidative damage after salt stress treatments. On the other hand, NtNAC028 mutation in tobacco resulted in upregulated expression of ROS-scavenging and abiotic stress-related genes, higher antioxidant enzyme activities, and enhanced tolerance against abiotic stresses, suggesting that NtNAC028 might act as a vital regulator for plant stress response likely by mediating ROS scavenging ability. Collectively, our results indicated that the NtNAC028 plays a key regulatory role in leaf senescence and response to multiple abiotic stresses. |
| first_indexed | 2024-04-13T13:16:30Z |
| format | Article |
| id | doaj.art-b9dd24a53cba4f99b6bea691c2e1dc18 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T13:16:30Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-b9dd24a53cba4f99b6bea691c2e1dc182022-12-22T02:45:26ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-08-011310.3389/fpls.2022.941026941026Characterization of NAC transcription factor NtNAC028 as a regulator of leaf senescence and stress responsesLichao WenTao LiuZhichao DengZenglin ZhangQi WangWeifeng WangWei LiYongfeng GuoNAC proteins constitute one of the largest transcription factor families and are involved in regulation of plant development and stress responses. Our previous transcriptome analyses of tobacco revealed a significant increase in the expression of NtNAC028 during leaf yellowing. In this study, we found that NtNAC028 was rapidly upregulated in response to high salinity, dehydration, and abscisic acid (ABA) stresses, suggesting a vital role of this gene in abiotic stress response. NtNAC028 loss-of-function tobacco plants generated via CRISPR-Cas9 showed delayed leaf senescence and increased tolerance to drought and salt stresses. Meanwhile NtNAC028 overexpression led to precocious leaf senescence and hypersensitivity to abiotic stresses in Arabidopsis, indicating that NtNAC028 functions as a positive regulator of natural leaf senescence and a negative regulator of stress tolerance. Furthermore, NtNAC028-overexpressing Arabidopsis plants showed lower antioxidant enzyme activities, higher reactive oxygen species (ROS), and H2O2 accumulation under high salinity, resulted in more severe oxidative damage after salt stress treatments. On the other hand, NtNAC028 mutation in tobacco resulted in upregulated expression of ROS-scavenging and abiotic stress-related genes, higher antioxidant enzyme activities, and enhanced tolerance against abiotic stresses, suggesting that NtNAC028 might act as a vital regulator for plant stress response likely by mediating ROS scavenging ability. Collectively, our results indicated that the NtNAC028 plays a key regulatory role in leaf senescence and response to multiple abiotic stresses.https://www.frontiersin.org/articles/10.3389/fpls.2022.941026/fulltobaccoNtNAC028abiotic stressleaf senescenceROSArabidopsis thaliana |
| spellingShingle | Lichao Wen Tao Liu Zhichao Deng Zenglin Zhang Qi Wang Weifeng Wang Wei Li Yongfeng Guo Characterization of NAC transcription factor NtNAC028 as a regulator of leaf senescence and stress responses Frontiers in Plant Science tobacco NtNAC028 abiotic stress leaf senescence ROS Arabidopsis thaliana |
| title | Characterization of NAC transcription factor NtNAC028 as a regulator of leaf senescence and stress responses |
| title_full | Characterization of NAC transcription factor NtNAC028 as a regulator of leaf senescence and stress responses |
| title_fullStr | Characterization of NAC transcription factor NtNAC028 as a regulator of leaf senescence and stress responses |
| title_full_unstemmed | Characterization of NAC transcription factor NtNAC028 as a regulator of leaf senescence and stress responses |
| title_short | Characterization of NAC transcription factor NtNAC028 as a regulator of leaf senescence and stress responses |
| title_sort | characterization of nac transcription factor ntnac028 as a regulator of leaf senescence and stress responses |
| topic | tobacco NtNAC028 abiotic stress leaf senescence ROS Arabidopsis thaliana |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.941026/full |
| work_keys_str_mv | AT lichaowen characterizationofnactranscriptionfactorntnac028asaregulatorofleafsenescenceandstressresponses AT taoliu characterizationofnactranscriptionfactorntnac028asaregulatorofleafsenescenceandstressresponses AT zhichaodeng characterizationofnactranscriptionfactorntnac028asaregulatorofleafsenescenceandstressresponses AT zenglinzhang characterizationofnactranscriptionfactorntnac028asaregulatorofleafsenescenceandstressresponses AT qiwang characterizationofnactranscriptionfactorntnac028asaregulatorofleafsenescenceandstressresponses AT weifengwang characterizationofnactranscriptionfactorntnac028asaregulatorofleafsenescenceandstressresponses AT weili characterizationofnactranscriptionfactorntnac028asaregulatorofleafsenescenceandstressresponses AT yongfengguo characterizationofnactranscriptionfactorntnac028asaregulatorofleafsenescenceandstressresponses |