Extending Single Cell Bioprinting from Femtosecond to Picosecond Laser Pulse Durations
Femtosecond laser pulses have been successfully used for film-free single-cell bioprinting, enabling precise and efficient selection and positioning of individual mammalian cells from a complex cell mixture (based on morphology or fluorescence) onto a 2D target substrate or a 3D pre-processed scaffo...
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MDPI AG
2021-09-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/12/10/1172 |
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| author | Jun Zhang Yasemin Geiger Florian Sotier Sasa Djordjevic Denitsa Docheva Stefanie Sudhop Hauke Clausen-Schaumann Heinz P. Huber |
| author_facet | Jun Zhang Yasemin Geiger Florian Sotier Sasa Djordjevic Denitsa Docheva Stefanie Sudhop Hauke Clausen-Schaumann Heinz P. Huber |
| author_sort | Jun Zhang |
| collection | DOAJ |
| description | Femtosecond laser pulses have been successfully used for film-free single-cell bioprinting, enabling precise and efficient selection and positioning of individual mammalian cells from a complex cell mixture (based on morphology or fluorescence) onto a 2D target substrate or a 3D pre-processed scaffold. In order to evaluate the effects of higher pulse durations on the bioprinting process, we investigated cavitation bubble and jet dynamics in the femto- and picosecond regime. By increasing the laser pulse duration from 600 fs to 14.1 ps, less energy is deposited in the hydrogel for the cavitation bubble expansion, resulting in less kinetic energy for the jet propagation with a slower jet velocity. Under appropriate conditions, single cells can be reliably transferred with a cell survival rate after transfer above 95% through the entire pulse duration range. More cost efficient and compact laser sources with pulse durations in the picosecond range could be used for film-free bioprinting and single-cell transfer. |
| first_indexed | 2024-03-10T06:23:19Z |
| format | Article |
| id | doaj.art-b9dd63bf0a3d4407851429815b4efd30 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T06:23:19Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-b9dd63bf0a3d4407851429815b4efd302023-11-22T19:10:55ZengMDPI AGMicromachines2072-666X2021-09-011210117210.3390/mi12101172Extending Single Cell Bioprinting from Femtosecond to Picosecond Laser Pulse DurationsJun Zhang0Yasemin Geiger1Florian Sotier2Sasa Djordjevic3Denitsa Docheva4Stefanie Sudhop5Hauke Clausen-Schaumann6Heinz P. Huber7Lasercenter, Department of Applied Sciences and Mechatronics, Munich University of Applied Sciences, Lothstrasse 34, 80335 Munich, GermanyLasercenter, Department of Applied Sciences and Mechatronics, Munich University of Applied Sciences, Lothstrasse 34, 80335 Munich, GermanyInnoLas Photonics GmbH, Justus-von-Liebig-Ring 8, 82152 Krailling, GermanyLasercenter, Department of Applied Sciences and Mechatronics, Munich University of Applied Sciences, Lothstrasse 34, 80335 Munich, GermanyExperimental Trauma Surgery, Department of Trauma Surgery, University Regensburg Medical Centre, Am Biopark 9, 93053 Regensburg, GermanyCenter for Applied Tissue Engineering and Regenerative Medicine CANTER, Munich University of Applied Sciences, Lothstrasse 34, 80335 Munich, GermanyCenter for Applied Tissue Engineering and Regenerative Medicine CANTER, Munich University of Applied Sciences, Lothstrasse 34, 80335 Munich, GermanyLasercenter, Department of Applied Sciences and Mechatronics, Munich University of Applied Sciences, Lothstrasse 34, 80335 Munich, GermanyFemtosecond laser pulses have been successfully used for film-free single-cell bioprinting, enabling precise and efficient selection and positioning of individual mammalian cells from a complex cell mixture (based on morphology or fluorescence) onto a 2D target substrate or a 3D pre-processed scaffold. In order to evaluate the effects of higher pulse durations on the bioprinting process, we investigated cavitation bubble and jet dynamics in the femto- and picosecond regime. By increasing the laser pulse duration from 600 fs to 14.1 ps, less energy is deposited in the hydrogel for the cavitation bubble expansion, resulting in less kinetic energy for the jet propagation with a slower jet velocity. Under appropriate conditions, single cells can be reliably transferred with a cell survival rate after transfer above 95% through the entire pulse duration range. More cost efficient and compact laser sources with pulse durations in the picosecond range could be used for film-free bioprinting and single-cell transfer.https://www.mdpi.com/2072-666X/12/10/1172laser-induced forward transfer (LIFT)film-free LIFTsingle-cell bioprintingtissue engineeringfemtosecond laser-based bioprintingpicosecond laser-based bioprinting |
| spellingShingle | Jun Zhang Yasemin Geiger Florian Sotier Sasa Djordjevic Denitsa Docheva Stefanie Sudhop Hauke Clausen-Schaumann Heinz P. Huber Extending Single Cell Bioprinting from Femtosecond to Picosecond Laser Pulse Durations Micromachines laser-induced forward transfer (LIFT) film-free LIFT single-cell bioprinting tissue engineering femtosecond laser-based bioprinting picosecond laser-based bioprinting |
| title | Extending Single Cell Bioprinting from Femtosecond to Picosecond Laser Pulse Durations |
| title_full | Extending Single Cell Bioprinting from Femtosecond to Picosecond Laser Pulse Durations |
| title_fullStr | Extending Single Cell Bioprinting from Femtosecond to Picosecond Laser Pulse Durations |
| title_full_unstemmed | Extending Single Cell Bioprinting from Femtosecond to Picosecond Laser Pulse Durations |
| title_short | Extending Single Cell Bioprinting from Femtosecond to Picosecond Laser Pulse Durations |
| title_sort | extending single cell bioprinting from femtosecond to picosecond laser pulse durations |
| topic | laser-induced forward transfer (LIFT) film-free LIFT single-cell bioprinting tissue engineering femtosecond laser-based bioprinting picosecond laser-based bioprinting |
| url | https://www.mdpi.com/2072-666X/12/10/1172 |
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