Development of an immunochromatographic point-of-care test for detection of IgG antibody in serodiagnosis of human trichinellosis
Objective: Trichinellosis is a globally distributed food-borne parasitic disease. Initially, diagnosis is usually made based on clinical signs, symptoms, and history of eating raw or undercooked meat. In the present study, an immunochromatographic test (ICT) kit was developed to diagnose trichinello...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-10-01
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| Series: | International Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971221006895 |
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| author | Tongjit Thanchomnang Lakkhana Sadaow Oranuch Sanpool Pewpan M. Intapan Rutchanee Rodpai Patcharaporn Boonroumkaew Penchom Janwan Somjintana Tourtip Wanchai Maleewong |
| author_facet | Tongjit Thanchomnang Lakkhana Sadaow Oranuch Sanpool Pewpan M. Intapan Rutchanee Rodpai Patcharaporn Boonroumkaew Penchom Janwan Somjintana Tourtip Wanchai Maleewong |
| author_sort | Tongjit Thanchomnang |
| collection | DOAJ |
| description | Objective: Trichinellosis is a globally distributed food-borne parasitic disease. Initially, diagnosis is usually made based on clinical signs, symptoms, and history of eating raw or undercooked meat. In the present study, an immunochromatographic test (ICT) kit was developed to diagnose trichinellosis by detecting IgG antibodies in sera of infected humans.Methods: Somatic extract from Trichinella spiralis larvae was used as the antigen for the ICT kit development. Diagnostic efficacy was evaluated using human serum samples from proven trichinellosis patients, healthy persons, and those with other parasitic infections.Results: The diagnostic sensitivity, specificity, positive and negative predictive values and accuracy of the ICT kit were 100.0% (95%; CI 88.8 to 100.0%), 92.5% (95% CI; 86.9 to 96.2%), 73.8% (95% CI; 58.0 to 86.1%), 100.0% (95% CI; 97.3 to 100.0%) and 93.8% (95% CI; 89.2 to 96.9%), respectively.Conclusions: This ICT diagnostic kit can be used as a testing tool for human trichinellosis. This test should permit rapid detection of infection to enable prompt anthelminthic treatment. It can also be used for retrospective diagnoses and field surveys based at laboratories where sophisticated equipment is lacking. |
| first_indexed | 2024-12-17T13:11:39Z |
| format | Article |
| id | doaj.art-b9dd763835cf4183a8a9fea55fd02bc7 |
| institution | Directory Open Access Journal |
| issn | 1201-9712 |
| language | English |
| last_indexed | 2024-12-17T13:11:39Z |
| publishDate | 2021-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-b9dd763835cf4183a8a9fea55fd02bc72022-12-21T21:47:06ZengElsevierInternational Journal of Infectious Diseases1201-97122021-10-01111148153Development of an immunochromatographic point-of-care test for detection of IgG antibody in serodiagnosis of human trichinellosisTongjit Thanchomnang0Lakkhana Sadaow1Oranuch Sanpool2Pewpan M. Intapan3Rutchanee Rodpai4Patcharaporn Boonroumkaew5Penchom Janwan6Somjintana Tourtip7Wanchai Maleewong8Faculty of Medicine, Mahasarakham University, Maha Sarakham 44000, Thailand; Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand; Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand; Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand; Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand; Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand; Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161, ThailandFaculty of Medicine, Mahasarakham University, Maha Sarakham 44000, ThailandDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand; Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand; Corresponding author: Wanchai Maleewong, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand ORCID iD: 0000-0002-6726-3759Objective: Trichinellosis is a globally distributed food-borne parasitic disease. Initially, diagnosis is usually made based on clinical signs, symptoms, and history of eating raw or undercooked meat. In the present study, an immunochromatographic test (ICT) kit was developed to diagnose trichinellosis by detecting IgG antibodies in sera of infected humans.Methods: Somatic extract from Trichinella spiralis larvae was used as the antigen for the ICT kit development. Diagnostic efficacy was evaluated using human serum samples from proven trichinellosis patients, healthy persons, and those with other parasitic infections.Results: The diagnostic sensitivity, specificity, positive and negative predictive values and accuracy of the ICT kit were 100.0% (95%; CI 88.8 to 100.0%), 92.5% (95% CI; 86.9 to 96.2%), 73.8% (95% CI; 58.0 to 86.1%), 100.0% (95% CI; 97.3 to 100.0%) and 93.8% (95% CI; 89.2 to 96.9%), respectively.Conclusions: This ICT diagnostic kit can be used as a testing tool for human trichinellosis. This test should permit rapid detection of infection to enable prompt anthelminthic treatment. It can also be used for retrospective diagnoses and field surveys based at laboratories where sophisticated equipment is lacking.http://www.sciencedirect.com/science/article/pii/S1201971221006895TrichinellosisTrichinellaDiagnosisImmunochromatographic testScreening testRapid test |
| spellingShingle | Tongjit Thanchomnang Lakkhana Sadaow Oranuch Sanpool Pewpan M. Intapan Rutchanee Rodpai Patcharaporn Boonroumkaew Penchom Janwan Somjintana Tourtip Wanchai Maleewong Development of an immunochromatographic point-of-care test for detection of IgG antibody in serodiagnosis of human trichinellosis International Journal of Infectious Diseases Trichinellosis Trichinella Diagnosis Immunochromatographic test Screening test Rapid test |
| title | Development of an immunochromatographic point-of-care test for detection of IgG antibody in serodiagnosis of human trichinellosis |
| title_full | Development of an immunochromatographic point-of-care test for detection of IgG antibody in serodiagnosis of human trichinellosis |
| title_fullStr | Development of an immunochromatographic point-of-care test for detection of IgG antibody in serodiagnosis of human trichinellosis |
| title_full_unstemmed | Development of an immunochromatographic point-of-care test for detection of IgG antibody in serodiagnosis of human trichinellosis |
| title_short | Development of an immunochromatographic point-of-care test for detection of IgG antibody in serodiagnosis of human trichinellosis |
| title_sort | development of an immunochromatographic point of care test for detection of igg antibody in serodiagnosis of human trichinellosis |
| topic | Trichinellosis Trichinella Diagnosis Immunochromatographic test Screening test Rapid test |
| url | http://www.sciencedirect.com/science/article/pii/S1201971221006895 |
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