Lab-on-Chip Platform for Culturing and Dynamic Evaluation of Cells Development
This paper presents a full-featured microfluidic platform ensuring long-term culturing and behavioral analysis of the radically different biological micro-objects. The platform uses all-glass lab-chips and MEMS-based components providing dedicated micro-aquatic habitats for the cells, as well as the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-02-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/11/2/196 |
| _version_ | 1828263867039350784 |
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| author | Agnieszka Podwin Danylo Lizanets Dawid Przystupski Wojciech Kubicki Patrycja Śniadek Julita Kulbacka Artur Wymysłowski Rafał Walczak Jan A. Dziuban |
| author_facet | Agnieszka Podwin Danylo Lizanets Dawid Przystupski Wojciech Kubicki Patrycja Śniadek Julita Kulbacka Artur Wymysłowski Rafał Walczak Jan A. Dziuban |
| author_sort | Agnieszka Podwin |
| collection | DOAJ |
| description | This paper presents a full-featured microfluidic platform ensuring long-term culturing and behavioral analysis of the radically different biological micro-objects. The platform uses all-glass lab-chips and MEMS-based components providing dedicated micro-aquatic habitats for the cells, as well as their intentional disturbances on-chip. Specially developed software was implemented to characterize the micro-objects metrologically in terms of population growth and cells’ size, shape, or migration activity. To date, the platform has been successfully applied for the culturing of freshwater microorganisms, fungi, cancer cells, and animal oocytes, showing their notable population growth, high mobility, and taxis mechanisms. For instance, circa 100% expansion of porcine oocytes cells, as well as nearly five-fold increase in <i>E. gracilis</i> population, has been achieved. These results are a good base to conduct further research on the platform versatile applications. |
| first_indexed | 2024-04-13T04:16:58Z |
| format | Article |
| id | doaj.art-b9de3b5385db4b0a9e5bbdfd6428cbe3 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-04-13T04:16:58Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-b9de3b5385db4b0a9e5bbdfd6428cbe32022-12-22T03:02:57ZengMDPI AGMicromachines2072-666X2020-02-0111219610.3390/mi11020196mi11020196Lab-on-Chip Platform for Culturing and Dynamic Evaluation of Cells DevelopmentAgnieszka Podwin0Danylo Lizanets1Dawid Przystupski2Wojciech Kubicki3Patrycja Śniadek4Julita Kulbacka5Artur Wymysłowski6Rafał Walczak7Jan A. Dziuban8Faculty of Microsystem Electronics and Photonics, Wrocław University of Science and Technology, 50-370 Wrocław, PolandFaculty of Microsystem Electronics and Photonics, Wrocław University of Science and Technology, 50-370 Wrocław, PolandDepartment of Molecular and Cellular Biology, Faculty of Pharmacy, Wrocław Medical University, 50-367 Wrocław, PolandFaculty of Microsystem Electronics and Photonics, Wrocław University of Science and Technology, 50-370 Wrocław, PolandFaculty of Microsystem Electronics and Photonics, Wrocław University of Science and Technology, 50-370 Wrocław, PolandDepartment of Molecular and Cellular Biology, Faculty of Pharmacy, Wrocław Medical University, 50-367 Wrocław, PolandFaculty of Microsystem Electronics and Photonics, Wrocław University of Science and Technology, 50-370 Wrocław, PolandFaculty of Microsystem Electronics and Photonics, Wrocław University of Science and Technology, 50-370 Wrocław, PolandFaculty of Microsystem Electronics and Photonics, Wrocław University of Science and Technology, 50-370 Wrocław, PolandThis paper presents a full-featured microfluidic platform ensuring long-term culturing and behavioral analysis of the radically different biological micro-objects. The platform uses all-glass lab-chips and MEMS-based components providing dedicated micro-aquatic habitats for the cells, as well as their intentional disturbances on-chip. Specially developed software was implemented to characterize the micro-objects metrologically in terms of population growth and cells’ size, shape, or migration activity. To date, the platform has been successfully applied for the culturing of freshwater microorganisms, fungi, cancer cells, and animal oocytes, showing their notable population growth, high mobility, and taxis mechanisms. For instance, circa 100% expansion of porcine oocytes cells, as well as nearly five-fold increase in <i>E. gracilis</i> population, has been achieved. These results are a good base to conduct further research on the platform versatile applications.https://www.mdpi.com/2072-666X/11/2/196lab-on-chip platformcell culturecancer researchivm (in vitro maturation)taxis effectsbehavioral analysis |
| spellingShingle | Agnieszka Podwin Danylo Lizanets Dawid Przystupski Wojciech Kubicki Patrycja Śniadek Julita Kulbacka Artur Wymysłowski Rafał Walczak Jan A. Dziuban Lab-on-Chip Platform for Culturing and Dynamic Evaluation of Cells Development Micromachines lab-on-chip platform cell culture cancer research ivm (in vitro maturation) taxis effects behavioral analysis |
| title | Lab-on-Chip Platform for Culturing and Dynamic Evaluation of Cells Development |
| title_full | Lab-on-Chip Platform for Culturing and Dynamic Evaluation of Cells Development |
| title_fullStr | Lab-on-Chip Platform for Culturing and Dynamic Evaluation of Cells Development |
| title_full_unstemmed | Lab-on-Chip Platform for Culturing and Dynamic Evaluation of Cells Development |
| title_short | Lab-on-Chip Platform for Culturing and Dynamic Evaluation of Cells Development |
| title_sort | lab on chip platform for culturing and dynamic evaluation of cells development |
| topic | lab-on-chip platform cell culture cancer research ivm (in vitro maturation) taxis effects behavioral analysis |
| url | https://www.mdpi.com/2072-666X/11/2/196 |
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