Effects of emergency/nonemergency cervical cerclage on the vaginal microbiome of pregnant women with cervical incompetence
BackgroundEvaluation of the therapeutic effects of cerclage on preterm birth (PTB) caused by cervical incompetence remains challenging. The vaginal microbiome is associated with preterm births. Thus, this study aimed to analyse the vaginal microbiota of patients with cervical incompetence, explore t...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1072960/full |
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| author | Yunshan Xiao Yunshan Xiao Yunshan Xiao Shiting Huang Shiting Huang Weiwei Yu Weiwei Yu Yan Ni Yan Ni Danni Lu Danni Lu Quanfeng Wu Quanfeng Wu Qin Leng Qin Leng Ting Yang Ting Yang Meilan Ni Meilan Ni Jingxian Xie Jingxian Xie Xueqin Zhang Xueqin Zhang Xueqin Zhang |
| author_facet | Yunshan Xiao Yunshan Xiao Yunshan Xiao Shiting Huang Shiting Huang Weiwei Yu Weiwei Yu Yan Ni Yan Ni Danni Lu Danni Lu Quanfeng Wu Quanfeng Wu Qin Leng Qin Leng Ting Yang Ting Yang Meilan Ni Meilan Ni Jingxian Xie Jingxian Xie Xueqin Zhang Xueqin Zhang Xueqin Zhang |
| author_sort | Yunshan Xiao |
| collection | DOAJ |
| description | BackgroundEvaluation of the therapeutic effects of cerclage on preterm birth (PTB) caused by cervical incompetence remains challenging. The vaginal microbiome is associated with preterm births. Thus, this study aimed to analyse the vaginal microbiota of patients with cervical incompetence, explore the relationship between the composition of the vaginal microbiota before cervical cerclage and at term delivery, and assess the effect of cervical cerclage on the vaginal microbiota.MethodsPatients (n = 30) underwent cerclage performed by the same surgical team. Vaginal swabs were obtained pre-surgery and seven days post-surgery. A gestational age-matched cohort of healthy pregnant women (n = 20) (no particular abnormality during pregnancy, delivery at term) was used as the control group and sampled during a comparable pregnancy. All collected vaginal swabs were analysed by 16S rRNA gene sequencing.ResultsWhen comparing the healthy control and cervical cerclage groups, the enriched microorganism in the healthy controls was G. Scardovia, and the enriched microorganism of the cerclage was G. Streptococcus. α diversity was significantly increased in patients who received cerclage with preterm delivery compared with those with full-term delivery, and the enriched microorganism was F. Enterococcus. A comparison before and after nonemergency cerclage suggested that the enriched microorganisms were G. Lactobacillus and F. Lactobacillaceae before surgery. After nonemergency cerclage, the enriched microorganisms were F. Enterobacteriaceae and C. Gammaproteobacteria. Vaginal microbiota diversity significantly increased, and the proportion of women with Lactobacillus spp.-depleted microbiomes increased after emergency cerclage. Significant differences in β diversity were found between the groups. Before the emergency cerclage, the enriched microorganisms were G. Lactobacillus, O. Alteromonadales, and P. Firmicutes. After emergency cerclage, the enriched microorganisms were P. Actinobacteria, C. Actinobacteria, P. Proteobacteria, F. Bifidobacteriaceae, O. Bifidobacteriales, G. Gardnerella, and G. Veillonella.ConclusionCerclage (particularly emergency cerclage) may alter the vaginal microbiota by increasing microbiota diversity, decreasing vaginal Lactobacillus abundance, and increasing the abundance of pathogenic bacteria that are not conducive to pregnancy maintenance, thereby affecting surgical efficacy. Therefore, the role of the vaginal microbiome should be considered when developing treatment strategies for pregnant women with cervical incompetence.Clinical trial registrationhttps://www.chictr.org.cn, identifier ChiCTR2100046305. |
| first_indexed | 2024-04-10T05:11:53Z |
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| last_indexed | 2024-04-10T05:11:53Z |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-b9e03bbdff2a4e25883d67fdd87440212023-03-09T07:17:20ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-03-011310.3389/fcimb.2023.10729601072960Effects of emergency/nonemergency cervical cerclage on the vaginal microbiome of pregnant women with cervical incompetenceYunshan Xiao0Yunshan Xiao1Yunshan Xiao2Shiting Huang3Shiting Huang4Weiwei Yu5Weiwei Yu6Yan Ni7Yan Ni8Danni Lu9Danni Lu10Quanfeng Wu11Quanfeng Wu12Qin Leng13Qin Leng14Ting Yang15Ting Yang16Meilan Ni17Meilan Ni18Jingxian Xie19Jingxian Xie20Xueqin Zhang21Xueqin Zhang22Xueqin Zhang23Department of Obstetrics, Women and Children’s Hospital, School of Medicine, Xiamen University, Xiamen, ChinaXiamen Key Laboratory of Basic and Clinical Research on Major Obstetrical Diseases, Xiamen, ChinaXiamen Clinical Research Center for Perinatal Medicine, Xiamen, ChinaDepartment of Obstetrics, Women and Children’s Hospital, School of Medicine, Xiamen University, Xiamen, ChinaXiamen Key Laboratory of Basic and Clinical Research on Major Obstetrical Diseases, Xiamen, ChinaDepartment of Obstetrics, Women and Children’s Hospital, School of Medicine, Xiamen University, Xiamen, ChinaXiamen Key Laboratory of Basic and Clinical Research on Major Obstetrical Diseases, Xiamen, ChinaDepartment of Obstetrics, Women and Children’s Hospital, School of Medicine, Xiamen University, Xiamen, ChinaXiamen Key Laboratory of Basic and Clinical Research on Major Obstetrical Diseases, Xiamen, ChinaDepartment of Obstetrics, Women and Children’s Hospital, School of Medicine, Xiamen University, Xiamen, ChinaXiamen Key Laboratory of Basic and Clinical Research on Major Obstetrical Diseases, Xiamen, ChinaDepartment of Obstetrics, Women and Children’s Hospital, School of Medicine, Xiamen University, Xiamen, ChinaXiamen Key Laboratory of Basic and Clinical Research on Major Obstetrical Diseases, Xiamen, ChinaDepartment of Obstetrics, Women and Children’s Hospital, School of Medicine, Xiamen University, Xiamen, ChinaXiamen Key Laboratory of Basic and Clinical Research on Major Obstetrical Diseases, Xiamen, ChinaDepartment of Obstetrics, Women and Children’s Hospital, School of Medicine, Xiamen University, Xiamen, ChinaXiamen Key Laboratory of Basic and Clinical Research on Major Obstetrical Diseases, Xiamen, ChinaDepartment of Obstetrics, Women and Children’s Hospital, School of Medicine, Xiamen University, Xiamen, ChinaXiamen Key Laboratory of Basic and Clinical Research on Major Obstetrical Diseases, Xiamen, ChinaDepartment of Obstetrics, Women and Children’s Hospital, School of Medicine, Xiamen University, Xiamen, ChinaXiamen Key Laboratory of Basic and Clinical Research on Major Obstetrical Diseases, Xiamen, ChinaDepartment of Obstetrics, Women and Children’s Hospital, School of Medicine, Xiamen University, Xiamen, ChinaXiamen Key Laboratory of Basic and Clinical Research on Major Obstetrical Diseases, Xiamen, ChinaXiamen Clinical Research Center for Perinatal Medicine, Xiamen, ChinaBackgroundEvaluation of the therapeutic effects of cerclage on preterm birth (PTB) caused by cervical incompetence remains challenging. The vaginal microbiome is associated with preterm births. Thus, this study aimed to analyse the vaginal microbiota of patients with cervical incompetence, explore the relationship between the composition of the vaginal microbiota before cervical cerclage and at term delivery, and assess the effect of cervical cerclage on the vaginal microbiota.MethodsPatients (n = 30) underwent cerclage performed by the same surgical team. Vaginal swabs were obtained pre-surgery and seven days post-surgery. A gestational age-matched cohort of healthy pregnant women (n = 20) (no particular abnormality during pregnancy, delivery at term) was used as the control group and sampled during a comparable pregnancy. All collected vaginal swabs were analysed by 16S rRNA gene sequencing.ResultsWhen comparing the healthy control and cervical cerclage groups, the enriched microorganism in the healthy controls was G. Scardovia, and the enriched microorganism of the cerclage was G. Streptococcus. α diversity was significantly increased in patients who received cerclage with preterm delivery compared with those with full-term delivery, and the enriched microorganism was F. Enterococcus. A comparison before and after nonemergency cerclage suggested that the enriched microorganisms were G. Lactobacillus and F. Lactobacillaceae before surgery. After nonemergency cerclage, the enriched microorganisms were F. Enterobacteriaceae and C. Gammaproteobacteria. Vaginal microbiota diversity significantly increased, and the proportion of women with Lactobacillus spp.-depleted microbiomes increased after emergency cerclage. Significant differences in β diversity were found between the groups. Before the emergency cerclage, the enriched microorganisms were G. Lactobacillus, O. Alteromonadales, and P. Firmicutes. After emergency cerclage, the enriched microorganisms were P. Actinobacteria, C. Actinobacteria, P. Proteobacteria, F. Bifidobacteriaceae, O. Bifidobacteriales, G. Gardnerella, and G. Veillonella.ConclusionCerclage (particularly emergency cerclage) may alter the vaginal microbiota by increasing microbiota diversity, decreasing vaginal Lactobacillus abundance, and increasing the abundance of pathogenic bacteria that are not conducive to pregnancy maintenance, thereby affecting surgical efficacy. Therefore, the role of the vaginal microbiome should be considered when developing treatment strategies for pregnant women with cervical incompetence.Clinical trial registrationhttps://www.chictr.org.cn, identifier ChiCTR2100046305.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1072960/fullcervical cerclagecervical incompetencevaginal microbiotaPTBpreterm birth |
| spellingShingle | Yunshan Xiao Yunshan Xiao Yunshan Xiao Shiting Huang Shiting Huang Weiwei Yu Weiwei Yu Yan Ni Yan Ni Danni Lu Danni Lu Quanfeng Wu Quanfeng Wu Qin Leng Qin Leng Ting Yang Ting Yang Meilan Ni Meilan Ni Jingxian Xie Jingxian Xie Xueqin Zhang Xueqin Zhang Xueqin Zhang Effects of emergency/nonemergency cervical cerclage on the vaginal microbiome of pregnant women with cervical incompetence Frontiers in Cellular and Infection Microbiology cervical cerclage cervical incompetence vaginal microbiota PTB preterm birth |
| title | Effects of emergency/nonemergency cervical cerclage on the vaginal microbiome of pregnant women with cervical incompetence |
| title_full | Effects of emergency/nonemergency cervical cerclage on the vaginal microbiome of pregnant women with cervical incompetence |
| title_fullStr | Effects of emergency/nonemergency cervical cerclage on the vaginal microbiome of pregnant women with cervical incompetence |
| title_full_unstemmed | Effects of emergency/nonemergency cervical cerclage on the vaginal microbiome of pregnant women with cervical incompetence |
| title_short | Effects of emergency/nonemergency cervical cerclage on the vaginal microbiome of pregnant women with cervical incompetence |
| title_sort | effects of emergency nonemergency cervical cerclage on the vaginal microbiome of pregnant women with cervical incompetence |
| topic | cervical cerclage cervical incompetence vaginal microbiota PTB preterm birth |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1072960/full |
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