Development of a Cryopreservation Technique for Xenogeneic Kidney Grafts: Evaluation Using a Mouse Model
To align the xeno-metanephros and renal progenitor cell timing for transplantation treatments, cryopreservation techniques and an efficient transportation of regenerated renal products such as xeno-metanephroi and renal progenitor cells should be established. Therefore, we propose a novel method of...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/11/23/7237 |
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| author | Tsuyoshi Takamura Hiroshi Nagashima Hitomi Matsunari Shuichiro Yamanaka Yatsumu Saito Yoshitaka Kinoshita Toshinari Fujimoto Kei Matsumoto Kazuaki Nakano Hirotaka James Okano Eiji Kobayashi Takashi Yokoo |
| author_facet | Tsuyoshi Takamura Hiroshi Nagashima Hitomi Matsunari Shuichiro Yamanaka Yatsumu Saito Yoshitaka Kinoshita Toshinari Fujimoto Kei Matsumoto Kazuaki Nakano Hirotaka James Okano Eiji Kobayashi Takashi Yokoo |
| author_sort | Tsuyoshi Takamura |
| collection | DOAJ |
| description | To align the xeno-metanephros and renal progenitor cell timing for transplantation treatments, cryopreservation techniques and an efficient transportation of regenerated renal products such as xeno-metanephroi and renal progenitor cells should be established. Therefore, we propose a novel method of xenogeneic regenerative medicine for patients with chronic kidney disease by grafting porcine fetal kidneys injected with human renal progenitor cells. To develop a useful cryopreserve system of porcine fetal kidney and human renal progenitor cells, we examined the cryopreservation of a fetal kidney implanted with renal progenitor cells in a mouse model. First, we developed a new method for direct cell injection under the capsule of the metanephros using gelatin as a support for unzipped fetal kidneys. Then, we confirmed in vitro that the nephrons derived from the transplanted cells were regenerated even after cryopreservation before and after cell transplantation. Furthermore, the cryopreserved chimeric metanephroi grew, and regenerated nephrons were observed in NOD. We confirmed that even in cryopreserved chimeric metanephroi, transplanted cell-derived nephrons as well as fresh transplants grew. |
| first_indexed | 2024-03-09T17:42:52Z |
| format | Article |
| id | doaj.art-b9e0b9d5e293454f998d6a63e577578b |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-09T17:42:52Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-b9e0b9d5e293454f998d6a63e577578b2023-11-24T11:25:16ZengMDPI AGJournal of Clinical Medicine2077-03832022-12-011123723710.3390/jcm11237237Development of a Cryopreservation Technique for Xenogeneic Kidney Grafts: Evaluation Using a Mouse ModelTsuyoshi Takamura0Hiroshi Nagashima1Hitomi Matsunari2Shuichiro Yamanaka3Yatsumu Saito4Yoshitaka Kinoshita5Toshinari Fujimoto6Kei Matsumoto7Kazuaki Nakano8Hirotaka James Okano9Eiji Kobayashi10Takashi Yokoo11Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanMeiji University International Institute for Bio-Resource Research, Kawasaki 214-8571, JapanMeiji University International Institute for Bio-Resource Research, Kawasaki 214-8571, JapanDivision of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanDivision of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanDivision of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanDivision of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanDivision of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanMeiji University International Institute for Bio-Resource Research, Kawasaki 214-8571, JapanDivision of Regenerative Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanDepartment of Kidney Regenerative Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanDivision of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanTo align the xeno-metanephros and renal progenitor cell timing for transplantation treatments, cryopreservation techniques and an efficient transportation of regenerated renal products such as xeno-metanephroi and renal progenitor cells should be established. Therefore, we propose a novel method of xenogeneic regenerative medicine for patients with chronic kidney disease by grafting porcine fetal kidneys injected with human renal progenitor cells. To develop a useful cryopreserve system of porcine fetal kidney and human renal progenitor cells, we examined the cryopreservation of a fetal kidney implanted with renal progenitor cells in a mouse model. First, we developed a new method for direct cell injection under the capsule of the metanephros using gelatin as a support for unzipped fetal kidneys. Then, we confirmed in vitro that the nephrons derived from the transplanted cells were regenerated even after cryopreservation before and after cell transplantation. Furthermore, the cryopreserved chimeric metanephroi grew, and regenerated nephrons were observed in NOD. We confirmed that even in cryopreserved chimeric metanephroi, transplanted cell-derived nephrons as well as fresh transplants grew.https://www.mdpi.com/2077-0383/11/23/7237kidney regenerationcryopreservationmetanephros |
| spellingShingle | Tsuyoshi Takamura Hiroshi Nagashima Hitomi Matsunari Shuichiro Yamanaka Yatsumu Saito Yoshitaka Kinoshita Toshinari Fujimoto Kei Matsumoto Kazuaki Nakano Hirotaka James Okano Eiji Kobayashi Takashi Yokoo Development of a Cryopreservation Technique for Xenogeneic Kidney Grafts: Evaluation Using a Mouse Model Journal of Clinical Medicine kidney regeneration cryopreservation metanephros |
| title | Development of a Cryopreservation Technique for Xenogeneic Kidney Grafts: Evaluation Using a Mouse Model |
| title_full | Development of a Cryopreservation Technique for Xenogeneic Kidney Grafts: Evaluation Using a Mouse Model |
| title_fullStr | Development of a Cryopreservation Technique for Xenogeneic Kidney Grafts: Evaluation Using a Mouse Model |
| title_full_unstemmed | Development of a Cryopreservation Technique for Xenogeneic Kidney Grafts: Evaluation Using a Mouse Model |
| title_short | Development of a Cryopreservation Technique for Xenogeneic Kidney Grafts: Evaluation Using a Mouse Model |
| title_sort | development of a cryopreservation technique for xenogeneic kidney grafts evaluation using a mouse model |
| topic | kidney regeneration cryopreservation metanephros |
| url | https://www.mdpi.com/2077-0383/11/23/7237 |
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