Integrated miRNA and mRNA Expression Profiles Reveal Differentially Expressed miR-222a as an Antiviral Factor Against Duck Hepatitis A Virus Type 1 Infection
Duck hepatitis A virus 1 (DHAV-1) is a highly contagious etiological agent that causes acute hepatitis in young ducklings. MicroRNAs (miRNAs) play important regulatory roles in response to pathogens. However, the interplay between DHAV-1 infection and miRNAs remains ambiguous. We characterized and c...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.811556/full |
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| author | Nana Sui Nana Sui Ruihua Zhang Ruihua Zhang Yue Jiang Yue Jiang Honglei Yu Honglei Yu Guige Xu Guige Xu Jingyu Wang Jingyu Wang Yanli Zhu Yanli Zhu Zhijing Xie Zhijing Xie Jiaqing Hu Jiaqing Hu Jiaqing Hu Shijin Jiang Shijin Jiang |
| author_facet | Nana Sui Nana Sui Ruihua Zhang Ruihua Zhang Yue Jiang Yue Jiang Honglei Yu Honglei Yu Guige Xu Guige Xu Jingyu Wang Jingyu Wang Yanli Zhu Yanli Zhu Zhijing Xie Zhijing Xie Jiaqing Hu Jiaqing Hu Jiaqing Hu Shijin Jiang Shijin Jiang |
| author_sort | Nana Sui |
| collection | DOAJ |
| description | Duck hepatitis A virus 1 (DHAV-1) is a highly contagious etiological agent that causes acute hepatitis in young ducklings. MicroRNAs (miRNAs) play important regulatory roles in response to pathogens. However, the interplay between DHAV-1 infection and miRNAs remains ambiguous. We characterized and compared miRNA and mRNA expression profiles in duck embryo fibroblasts cells (DEFs) infected with DHAV-1. In total, 36 and 96 differentially expressed (DE) miRNAs, and 4110 and 2595 DE mRNAs, were identified at 12 and 24 h after infection. In particular, 126 and 275 miRNA–mRNA pairs with a negative correlation were chosen to construct an interaction network. Subsequently, we identified the functional annotation of DE mRNAs and target genes of DE miRNAs enriched in diverse Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, which may be important for virus resistance, cell proliferation, and metabolism. Moreover, upregulated miR-222a could negatively regulate DHAV-1 replication in DEFs and downregulate integrin subunit beta 3 (ITGB3) expression by targeting the 3′ untranslated region (3′UTR), indicating that miR-222a may modulate DHAV-1 replication via interaction with ITGB3. In conclusion, the results reveal changes of mRNAs and miRNAs during DHAV-1 infection and suggest miR-222a as an antiviral factor against DHAV-1. |
| first_indexed | 2024-12-22T20:28:14Z |
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| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-22T20:28:14Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-b9e1c968aeee4b7c81d6e4917a6f97712022-12-21T18:13:40ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-01-011110.3389/fcimb.2021.811556811556Integrated miRNA and mRNA Expression Profiles Reveal Differentially Expressed miR-222a as an Antiviral Factor Against Duck Hepatitis A Virus Type 1 InfectionNana Sui0Nana Sui1Ruihua Zhang2Ruihua Zhang3Yue Jiang4Yue Jiang5Honglei Yu6Honglei Yu7Guige Xu8Guige Xu9Jingyu Wang10Jingyu Wang11Yanli Zhu12Yanli Zhu13Zhijing Xie14Zhijing Xie15Jiaqing Hu16Jiaqing Hu17Jiaqing Hu18Shijin Jiang19Shijin Jiang20College of Veterinary Medicine, Shandong Agricultural University, Taian, ChinaShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Taian, ChinaShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Taian, ChinaShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Taian, ChinaShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Taian, ChinaShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Taian, ChinaShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Taian, ChinaShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Taian, ChinaShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Taian, ChinaShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian, ChinaShandong GreenBlue Biotechnology Co. Ltd. Economic Development Zone, Ningyang County, Taian, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Taian, ChinaShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian, ChinaDuck hepatitis A virus 1 (DHAV-1) is a highly contagious etiological agent that causes acute hepatitis in young ducklings. MicroRNAs (miRNAs) play important regulatory roles in response to pathogens. However, the interplay between DHAV-1 infection and miRNAs remains ambiguous. We characterized and compared miRNA and mRNA expression profiles in duck embryo fibroblasts cells (DEFs) infected with DHAV-1. In total, 36 and 96 differentially expressed (DE) miRNAs, and 4110 and 2595 DE mRNAs, were identified at 12 and 24 h after infection. In particular, 126 and 275 miRNA–mRNA pairs with a negative correlation were chosen to construct an interaction network. Subsequently, we identified the functional annotation of DE mRNAs and target genes of DE miRNAs enriched in diverse Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, which may be important for virus resistance, cell proliferation, and metabolism. Moreover, upregulated miR-222a could negatively regulate DHAV-1 replication in DEFs and downregulate integrin subunit beta 3 (ITGB3) expression by targeting the 3′ untranslated region (3′UTR), indicating that miR-222a may modulate DHAV-1 replication via interaction with ITGB3. In conclusion, the results reveal changes of mRNAs and miRNAs during DHAV-1 infection and suggest miR-222a as an antiviral factor against DHAV-1.https://www.frontiersin.org/articles/10.3389/fcimb.2021.811556/fullDHAV-1miRNAmRNAdifferential expressioninteraction network3′UTR |
| spellingShingle | Nana Sui Nana Sui Ruihua Zhang Ruihua Zhang Yue Jiang Yue Jiang Honglei Yu Honglei Yu Guige Xu Guige Xu Jingyu Wang Jingyu Wang Yanli Zhu Yanli Zhu Zhijing Xie Zhijing Xie Jiaqing Hu Jiaqing Hu Jiaqing Hu Shijin Jiang Shijin Jiang Integrated miRNA and mRNA Expression Profiles Reveal Differentially Expressed miR-222a as an Antiviral Factor Against Duck Hepatitis A Virus Type 1 Infection Frontiers in Cellular and Infection Microbiology DHAV-1 miRNA mRNA differential expression interaction network 3′UTR |
| title | Integrated miRNA and mRNA Expression Profiles Reveal Differentially Expressed miR-222a as an Antiviral Factor Against Duck Hepatitis A Virus Type 1 Infection |
| title_full | Integrated miRNA and mRNA Expression Profiles Reveal Differentially Expressed miR-222a as an Antiviral Factor Against Duck Hepatitis A Virus Type 1 Infection |
| title_fullStr | Integrated miRNA and mRNA Expression Profiles Reveal Differentially Expressed miR-222a as an Antiviral Factor Against Duck Hepatitis A Virus Type 1 Infection |
| title_full_unstemmed | Integrated miRNA and mRNA Expression Profiles Reveal Differentially Expressed miR-222a as an Antiviral Factor Against Duck Hepatitis A Virus Type 1 Infection |
| title_short | Integrated miRNA and mRNA Expression Profiles Reveal Differentially Expressed miR-222a as an Antiviral Factor Against Duck Hepatitis A Virus Type 1 Infection |
| title_sort | integrated mirna and mrna expression profiles reveal differentially expressed mir 222a as an antiviral factor against duck hepatitis a virus type 1 infection |
| topic | DHAV-1 miRNA mRNA differential expression interaction network 3′UTR |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2021.811556/full |
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