Label-Free Surface-Enhanced Raman Spectroscopic Analysis of Proteins: Advances and Applications
Surface-enhanced Raman spectroscopy (SERS) is powerful for structural characterization of biomolecules under physiological condition. Owing to its high sensitivity and selectivity, SERS is useful for probing intrinsic structural information of proteins and is attracting increasing attention in bioph...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/22/13868 |
| _version_ | 1797465190558597120 |
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| author | Linjun Cai Guilin Fang Jinpin Tang Qiaomei Cheng Xiaoxia Han |
| author_facet | Linjun Cai Guilin Fang Jinpin Tang Qiaomei Cheng Xiaoxia Han |
| author_sort | Linjun Cai |
| collection | DOAJ |
| description | Surface-enhanced Raman spectroscopy (SERS) is powerful for structural characterization of biomolecules under physiological condition. Owing to its high sensitivity and selectivity, SERS is useful for probing intrinsic structural information of proteins and is attracting increasing attention in biophysics, bioanalytical chemistry, and biomedicine. This review starts with a brief introduction of SERS theories and SERS methodology of protein structural characterization. SERS-active materials, related synthetic approaches, and strategies for protein-material assemblies are outlined and discussed, followed by detailed discussion of SERS spectroscopy of proteins with and without cofactors. Recent applications and advances of protein SERS in biomarker detection, cell analysis, and pathogen discrimination are then highlighted, and the spectral reproducibility and limitations are critically discussed. The review ends with a conclusion and a discussion of current challenges and perspectives of promising directions. |
| first_indexed | 2024-03-09T18:17:58Z |
| format | Article |
| id | doaj.art-b9e2b91ace274028aef78fcb8636d13a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T18:17:58Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b9e2b91ace274028aef78fcb8636d13a2023-11-24T08:34:39ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123221386810.3390/ijms232213868Label-Free Surface-Enhanced Raman Spectroscopic Analysis of Proteins: Advances and ApplicationsLinjun Cai0Guilin Fang1Jinpin Tang2Qiaomei Cheng3Xiaoxia Han4National Engineering Laboratory for AIDS Vaccine, School of Life Science, Jilin University, Changchun 130012, ChinaNational Engineering Laboratory for AIDS Vaccine, School of Life Science, Jilin University, Changchun 130012, ChinaState Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, ChinaNational Engineering Laboratory for AIDS Vaccine, School of Life Science, Jilin University, Changchun 130012, ChinaState Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, ChinaSurface-enhanced Raman spectroscopy (SERS) is powerful for structural characterization of biomolecules under physiological condition. Owing to its high sensitivity and selectivity, SERS is useful for probing intrinsic structural information of proteins and is attracting increasing attention in biophysics, bioanalytical chemistry, and biomedicine. This review starts with a brief introduction of SERS theories and SERS methodology of protein structural characterization. SERS-active materials, related synthetic approaches, and strategies for protein-material assemblies are outlined and discussed, followed by detailed discussion of SERS spectroscopy of proteins with and without cofactors. Recent applications and advances of protein SERS in biomarker detection, cell analysis, and pathogen discrimination are then highlighted, and the spectral reproducibility and limitations are critically discussed. The review ends with a conclusion and a discussion of current challenges and perspectives of promising directions.https://www.mdpi.com/1422-0067/23/22/13868SERSproteinstructural characterizationbiomarkercellpathogen |
| spellingShingle | Linjun Cai Guilin Fang Jinpin Tang Qiaomei Cheng Xiaoxia Han Label-Free Surface-Enhanced Raman Spectroscopic Analysis of Proteins: Advances and Applications International Journal of Molecular Sciences SERS protein structural characterization biomarker cell pathogen |
| title | Label-Free Surface-Enhanced Raman Spectroscopic Analysis of Proteins: Advances and Applications |
| title_full | Label-Free Surface-Enhanced Raman Spectroscopic Analysis of Proteins: Advances and Applications |
| title_fullStr | Label-Free Surface-Enhanced Raman Spectroscopic Analysis of Proteins: Advances and Applications |
| title_full_unstemmed | Label-Free Surface-Enhanced Raman Spectroscopic Analysis of Proteins: Advances and Applications |
| title_short | Label-Free Surface-Enhanced Raman Spectroscopic Analysis of Proteins: Advances and Applications |
| title_sort | label free surface enhanced raman spectroscopic analysis of proteins advances and applications |
| topic | SERS protein structural characterization biomarker cell pathogen |
| url | https://www.mdpi.com/1422-0067/23/22/13868 |
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