| Samenvatting: | To study the constituents of Isodon macrocalyx, thirteen diterpenoids were isolated and purified from the aerial parts of I. macrocalyx by means of various column chromatographic techniques, including silica gel, ODS, Sephadex LH-20 and RP-C18 pre-HPLC. The structures of the isolated diterpenoids were determined on the basis of analyses of spectroscopic methods (1H NMR and 13C NMR spectroscopy), high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), and comparison of their spectroscopic data with previously reported data. The results showed that the diterpenoids were identified as 19-hydroxytotarol (1), macrophynin E (2), inumakoic acid (3), inumakiol D (4), 4β-carboxy-19-nortotarol (5), (-)-lambertic acid (6), 2-oxo-5-fagonene (7), isodoterniofiln B (8), longikaurin E (9), longikaurin A (10), rabdotemin H (11), 16S-dihydrolongikaurin A (12), and ent-3S,16S,17-trihydroxy-kauran-2-one (13). All diterpenoids were isolated from I. macrocalyx for the first time.
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