Isolation of Cell-Free miRNA from Biological Fluids: Influencing Factors and Methods
A vast wealth of recent research has seen attempts of using microRNA (miRNA) found in biological fluids in clinical research and medicine. One of the reasons behind this trend is the apparent their high stability of cell-free miRNA conferred by small size and packaging in supramolecular complexes. H...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | Diagnostics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4418/11/5/865 |
| _version_ | 1797534543275622400 |
|---|---|
| author | Olga Bryzgunova Maria Konoshenko Ivan Zaporozhchenko Alexey Yakovlev Pavel Laktionov |
| author_facet | Olga Bryzgunova Maria Konoshenko Ivan Zaporozhchenko Alexey Yakovlev Pavel Laktionov |
| author_sort | Olga Bryzgunova |
| collection | DOAJ |
| description | A vast wealth of recent research has seen attempts of using microRNA (miRNA) found in biological fluids in clinical research and medicine. One of the reasons behind this trend is the apparent their high stability of cell-free miRNA conferred by small size and packaging in supramolecular complexes. However, researchers in both basic and clinical settings often face the problem of selecting adequate methods to extract appropriate quality miRNA preparations for use in specific downstream analysis pipelines. This review outlines the variety of different methods of miRNA isolation from biofluids and examines the key determinants of their efficiency, including, but not limited to, the structural properties of miRNA and factors defining their stability in the extracellular environment. |
| first_indexed | 2024-03-10T11:32:02Z |
| format | Article |
| id | doaj.art-b9ed0c32b4b34bb3bb7ec1c9aae6a24b |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T11:32:02Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-b9ed0c32b4b34bb3bb7ec1c9aae6a24b2023-11-21T19:13:00ZengMDPI AGDiagnostics2075-44182021-05-0111586510.3390/diagnostics11050865Isolation of Cell-Free miRNA from Biological Fluids: Influencing Factors and MethodsOlga Bryzgunova0Maria Konoshenko1Ivan Zaporozhchenko2Alexey Yakovlev3Pavel Laktionov4Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, 630090 Novosibirsk, RussiaDepartment of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, DenmarkInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, 630090 Novosibirsk, RussiaA vast wealth of recent research has seen attempts of using microRNA (miRNA) found in biological fluids in clinical research and medicine. One of the reasons behind this trend is the apparent their high stability of cell-free miRNA conferred by small size and packaging in supramolecular complexes. However, researchers in both basic and clinical settings often face the problem of selecting adequate methods to extract appropriate quality miRNA preparations for use in specific downstream analysis pipelines. This review outlines the variety of different methods of miRNA isolation from biofluids and examines the key determinants of their efficiency, including, but not limited to, the structural properties of miRNA and factors defining their stability in the extracellular environment.https://www.mdpi.com/2075-4418/11/5/865extracellular vesicleslipoproteinsbinding to blood cellscell-free miRNA structuremiRNA storageisolation kit |
| spellingShingle | Olga Bryzgunova Maria Konoshenko Ivan Zaporozhchenko Alexey Yakovlev Pavel Laktionov Isolation of Cell-Free miRNA from Biological Fluids: Influencing Factors and Methods Diagnostics extracellular vesicles lipoproteins binding to blood cells cell-free miRNA structure miRNA storage isolation kit |
| title | Isolation of Cell-Free miRNA from Biological Fluids: Influencing Factors and Methods |
| title_full | Isolation of Cell-Free miRNA from Biological Fluids: Influencing Factors and Methods |
| title_fullStr | Isolation of Cell-Free miRNA from Biological Fluids: Influencing Factors and Methods |
| title_full_unstemmed | Isolation of Cell-Free miRNA from Biological Fluids: Influencing Factors and Methods |
| title_short | Isolation of Cell-Free miRNA from Biological Fluids: Influencing Factors and Methods |
| title_sort | isolation of cell free mirna from biological fluids influencing factors and methods |
| topic | extracellular vesicles lipoproteins binding to blood cells cell-free miRNA structure miRNA storage isolation kit |
| url | https://www.mdpi.com/2075-4418/11/5/865 |
| work_keys_str_mv | AT olgabryzgunova isolationofcellfreemirnafrombiologicalfluidsinfluencingfactorsandmethods AT mariakonoshenko isolationofcellfreemirnafrombiologicalfluidsinfluencingfactorsandmethods AT ivanzaporozhchenko isolationofcellfreemirnafrombiologicalfluidsinfluencingfactorsandmethods AT alexeyyakovlev isolationofcellfreemirnafrombiologicalfluidsinfluencingfactorsandmethods AT pavellaktionov isolationofcellfreemirnafrombiologicalfluidsinfluencingfactorsandmethods |