Identification of faecal Escherichia coli isolates with similar patterns of virulence and antimicrobial resistance genes in dogs and their owners
Abstract Background The presence of antimicrobial resistance and virulence genes in Escherichia coli allows them to survive and cause infections. The close contact between humans and pets can reinforce the risk of transmitting resistant and virulent bacteria between them. Objectives This study aims...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.965 |
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| author | Zahra Naziri Abdollah Derakhshandeh Sahar Zare Malihe Akbarzadeh Niaki Azar Motamedi Boroojeni Vida Eraghi Abolfazl Shirmohamadi Sosfad |
| author_facet | Zahra Naziri Abdollah Derakhshandeh Sahar Zare Malihe Akbarzadeh Niaki Azar Motamedi Boroojeni Vida Eraghi Abolfazl Shirmohamadi Sosfad |
| author_sort | Zahra Naziri |
| collection | DOAJ |
| description | Abstract Background The presence of antimicrobial resistance and virulence genes in Escherichia coli allows them to survive and cause infections. The close contact between humans and pets can reinforce the risk of transmitting resistant and virulent bacteria between them. Objectives This study aims to compare the patterns of the presence of tetracycline and streptomycin resistance genes, as well as important virulence genes in E. coli isolated from faeces of healthy dogs and their owners. Methods Polymerase chain reactions were performed for detection of antimicrobial resistance (tetA, tetB, tetC, tetD, strA and strB) and virulence (fimH, iss, sitA and malX) genes in 144 faecal E. coli isolates from 28 dog–owner pairs and 16 humans who did not keep any pets as controls. Results Among the investigated antimicrobial resistance and virulence genes, tetA (52.1%) and fimH (86.8%) genes had the highest prevalence. No statistically significant difference was found between the prevalence of antimicrobial resistance and virulence genes in isolates of dogs and their owners. In total, 46.4% of dog–owner pairs had the same patterns of presence or absence of six antimicrobial resistance genes, 50.0% had the same patterns of presence or absence of four virulence genes and 25.0% had the same patterns of presence or absence of all 10 tested genes. Conclusion The presence of antimicrobial‐resistant virulent E. coli in humans and pets may predispose them to infections that are hard to cure with conventional antibiotics. Notable frequency of dogs’ and their owners’ E. coli isolates with similar patterns of antimicrobial resistance and virulence genes may indicate the possibility of sharing virulent antimicrobial resistant E. coli between them. |
| first_indexed | 2024-04-10T21:13:28Z |
| format | Article |
| id | doaj.art-b9f0e83127804b90b65ab292fe62efd4 |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-04-10T21:13:28Z |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-b9f0e83127804b90b65ab292fe62efd42023-01-20T17:53:08ZengWileyVeterinary Medicine and Science2053-10952023-01-019112613110.1002/vms3.965Identification of faecal Escherichia coli isolates with similar patterns of virulence and antimicrobial resistance genes in dogs and their ownersZahra Naziri0Abdollah Derakhshandeh1Sahar Zare2Malihe Akbarzadeh Niaki3Azar Motamedi Boroojeni4Vida Eraghi5Abolfazl Shirmohamadi Sosfad6Department of Pathobiology, School of Veterinary Medicine Shiraz University Shiraz IranDepartment of Pathobiology, School of Veterinary Medicine Shiraz University Shiraz IranDepartment of Pathobiology, School of Veterinary Medicine Shiraz University Shiraz IranDepartment of Pathobiology, School of Veterinary Medicine Shiraz University Shiraz IranDepartment of Pathobiology, School of Veterinary Medicine Shiraz University Shiraz IranDepartment of Pathobiology, School of Veterinary Medicine Shiraz University Shiraz IranDepartment of Pathobiology, School of Veterinary Medicine Shiraz University Shiraz IranAbstract Background The presence of antimicrobial resistance and virulence genes in Escherichia coli allows them to survive and cause infections. The close contact between humans and pets can reinforce the risk of transmitting resistant and virulent bacteria between them. Objectives This study aims to compare the patterns of the presence of tetracycline and streptomycin resistance genes, as well as important virulence genes in E. coli isolated from faeces of healthy dogs and their owners. Methods Polymerase chain reactions were performed for detection of antimicrobial resistance (tetA, tetB, tetC, tetD, strA and strB) and virulence (fimH, iss, sitA and malX) genes in 144 faecal E. coli isolates from 28 dog–owner pairs and 16 humans who did not keep any pets as controls. Results Among the investigated antimicrobial resistance and virulence genes, tetA (52.1%) and fimH (86.8%) genes had the highest prevalence. No statistically significant difference was found between the prevalence of antimicrobial resistance and virulence genes in isolates of dogs and their owners. In total, 46.4% of dog–owner pairs had the same patterns of presence or absence of six antimicrobial resistance genes, 50.0% had the same patterns of presence or absence of four virulence genes and 25.0% had the same patterns of presence or absence of all 10 tested genes. Conclusion The presence of antimicrobial‐resistant virulent E. coli in humans and pets may predispose them to infections that are hard to cure with conventional antibiotics. Notable frequency of dogs’ and their owners’ E. coli isolates with similar patterns of antimicrobial resistance and virulence genes may indicate the possibility of sharing virulent antimicrobial resistant E. coli between them.https://doi.org/10.1002/vms3.965dogdog ownerE. colistreptomycin resistance genestetracycline resistance genesvirulence gene |
| spellingShingle | Zahra Naziri Abdollah Derakhshandeh Sahar Zare Malihe Akbarzadeh Niaki Azar Motamedi Boroojeni Vida Eraghi Abolfazl Shirmohamadi Sosfad Identification of faecal Escherichia coli isolates with similar patterns of virulence and antimicrobial resistance genes in dogs and their owners Veterinary Medicine and Science dog dog owner E. coli streptomycin resistance genes tetracycline resistance genes virulence gene |
| title | Identification of faecal Escherichia coli isolates with similar patterns of virulence and antimicrobial resistance genes in dogs and their owners |
| title_full | Identification of faecal Escherichia coli isolates with similar patterns of virulence and antimicrobial resistance genes in dogs and their owners |
| title_fullStr | Identification of faecal Escherichia coli isolates with similar patterns of virulence and antimicrobial resistance genes in dogs and their owners |
| title_full_unstemmed | Identification of faecal Escherichia coli isolates with similar patterns of virulence and antimicrobial resistance genes in dogs and their owners |
| title_short | Identification of faecal Escherichia coli isolates with similar patterns of virulence and antimicrobial resistance genes in dogs and their owners |
| title_sort | identification of faecal escherichia coli isolates with similar patterns of virulence and antimicrobial resistance genes in dogs and their owners |
| topic | dog dog owner E. coli streptomycin resistance genes tetracycline resistance genes virulence gene |
| url | https://doi.org/10.1002/vms3.965 |
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