I329L protein-based indirect ELISA for detecting antibodies specific to African swine fever virus
African swine fever (ASF) is a disease that causes severe economic losses to the global porcine industry. As no vaccine or drug has been discovered for the prevention and control of ASF virus (ASFV), accurate diagnosis and timely eradication of infected animals are the primary measures, which necess...
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| Format: | Article |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1150042/full |
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| author | Zhiyong Shen Zhiyong Shen Wenchen Qiu Haorui Luan Chunxi Sun Xinya Cao Gang Wang Gang Wang Jun Peng Jun Peng |
| author_facet | Zhiyong Shen Zhiyong Shen Wenchen Qiu Haorui Luan Chunxi Sun Xinya Cao Gang Wang Gang Wang Jun Peng Jun Peng |
| author_sort | Zhiyong Shen |
| collection | DOAJ |
| description | African swine fever (ASF) is a disease that causes severe economic losses to the global porcine industry. As no vaccine or drug has been discovered for the prevention and control of ASF virus (ASFV), accurate diagnosis and timely eradication of infected animals are the primary measures, which necessitate accurate and effective detection methods. In this study, the truncated ASFV I329L (amino acids 70–237), was induced using IPTG and expressed in Escherichia coli cells. The highly antigenic viral protein I329L was used to develop an indirect enzyme-linked immunosorbent assay (iELISA), named I329L-ELISA, which cut-off value was 0.384. I329L-ELISA was used to detect 186 clinical pig serum samples, and the coincidence rate between the indirect ELISA developed here and the commercial kit was 96.77%. No cross-reactivity was observed with CSFV, PRRSV, PCV2, or PRV antibody-positive pig sera, indicating good specificity. Both intra- assay and inter-assay coefficients were below 10%, and the detection sensitivity of the iELISA reached 1:3200. In this study, an iELISA for ASFV antibody detection was developed based on the truncated ASFV I329L protein. Overall, the I329L-ELISA is a user-friendly detection tool that is suitable for ASFV antibody detection and epidemiological surveillance. |
| first_indexed | 2024-03-13T06:58:15Z |
| format | Article |
| id | doaj.art-b9f420768a0741b89c877c7c1fadf9f8 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-03-13T06:58:15Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-b9f420768a0741b89c877c7c1fadf9f82023-06-07T05:13:50ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-06-011310.3389/fcimb.2023.11500421150042I329L protein-based indirect ELISA for detecting antibodies specific to African swine fever virusZhiyong Shen0Zhiyong Shen1Wenchen Qiu2Haorui Luan3Chunxi Sun4Xinya Cao5Gang Wang6Gang Wang7Jun Peng8Jun Peng9College of Veterinary Medicine, Shandong Agricultural University, Tai’an, ChinaBreeding Management Department, Beisanxia Husbandry Company Limited, Hegang, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Tai’an, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Tai’an, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Tai’an, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Tai’an, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Tai’an, ChinaShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai’an, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Tai’an, ChinaShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai’an, ChinaAfrican swine fever (ASF) is a disease that causes severe economic losses to the global porcine industry. As no vaccine or drug has been discovered for the prevention and control of ASF virus (ASFV), accurate diagnosis and timely eradication of infected animals are the primary measures, which necessitate accurate and effective detection methods. In this study, the truncated ASFV I329L (amino acids 70–237), was induced using IPTG and expressed in Escherichia coli cells. The highly antigenic viral protein I329L was used to develop an indirect enzyme-linked immunosorbent assay (iELISA), named I329L-ELISA, which cut-off value was 0.384. I329L-ELISA was used to detect 186 clinical pig serum samples, and the coincidence rate between the indirect ELISA developed here and the commercial kit was 96.77%. No cross-reactivity was observed with CSFV, PRRSV, PCV2, or PRV antibody-positive pig sera, indicating good specificity. Both intra- assay and inter-assay coefficients were below 10%, and the detection sensitivity of the iELISA reached 1:3200. In this study, an iELISA for ASFV antibody detection was developed based on the truncated ASFV I329L protein. Overall, the I329L-ELISA is a user-friendly detection tool that is suitable for ASFV antibody detection and epidemiological surveillance.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1150042/fullAfrican swine fever virusI329L proteinprokaryotic expressionindirect ELISAantibody detection |
| spellingShingle | Zhiyong Shen Zhiyong Shen Wenchen Qiu Haorui Luan Chunxi Sun Xinya Cao Gang Wang Gang Wang Jun Peng Jun Peng I329L protein-based indirect ELISA for detecting antibodies specific to African swine fever virus Frontiers in Cellular and Infection Microbiology African swine fever virus I329L protein prokaryotic expression indirect ELISA antibody detection |
| title | I329L protein-based indirect ELISA for detecting antibodies specific to African swine fever virus |
| title_full | I329L protein-based indirect ELISA for detecting antibodies specific to African swine fever virus |
| title_fullStr | I329L protein-based indirect ELISA for detecting antibodies specific to African swine fever virus |
| title_full_unstemmed | I329L protein-based indirect ELISA for detecting antibodies specific to African swine fever virus |
| title_short | I329L protein-based indirect ELISA for detecting antibodies specific to African swine fever virus |
| title_sort | i329l protein based indirect elisa for detecting antibodies specific to african swine fever virus |
| topic | African swine fever virus I329L protein prokaryotic expression indirect ELISA antibody detection |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1150042/full |
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