NEK7 Regulates NLRP3 Inflammasome Activation and Neuroinflammation Post-traumatic Brain Injury
As one of the most common causes of mortality and disability, traumatic brain injury (TBI) is a huge psychological and economic burden to patients, families, and societies worldwide. Neuroinflammation reduction may be a favorable option to alleviate secondary brain injuries and ameliorate the outcom...
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| Format: | Article |
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Frontiers Media S.A.
2019-08-01
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| Series: | Frontiers in Molecular Neuroscience |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fnmol.2019.00202/full |
| _version_ | 1828421657056772096 |
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| author | Yuhua Chen Yuhua Chen Jiao Meng Jiao Meng Fangfang Bi Fangfang Bi Hua Li Cuicui Chang Chen Ji Wei Liu |
| author_facet | Yuhua Chen Yuhua Chen Jiao Meng Jiao Meng Fangfang Bi Fangfang Bi Hua Li Cuicui Chang Chen Ji Wei Liu |
| author_sort | Yuhua Chen |
| collection | DOAJ |
| description | As one of the most common causes of mortality and disability, traumatic brain injury (TBI) is a huge psychological and economic burden to patients, families, and societies worldwide. Neuroinflammation reduction may be a favorable option to alleviate secondary brain injuries and ameliorate the outcome of TBI. The nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain-containing 3 (NLRP3) inflammasome, has been shown to be involved in TBI. NIMA-related kinase 7 (NEK7) has been verified as an essential mediator of NLRP3 inflammasome activation that is recruited upstream of the formation of inflammasomes in response to NLRP3 activators. However, the underlying mechanism by which NEK7 operates post-TBI remains undefined. In this study, we performed both in vivo and in vitro experiments. Using an in vivo mouse TBI model, mice were administered an intracerebroventricular injection of NEK7-shRNA virus. For the in vitro analysis, primary cortical neurons with NEK7-shRNA were stimulated with lipopolysaccharide (LPS)/ATP or potassium (K+). We evaluated the effects of NEK7 knock-down on neurological deficits, NLRP3 inflammasomes, caspase-1 activation, and neuronal injury. During the 0–168 h post-TBI period in vivo, NEK7 and NLRP3 inflammasome activation increased in what appeared to be a time-dependent manner. As well as pyroptosis-related markers, caspase-1 activation (p20) and interleukin-1β (IL-1β) activation (p17) were up-regulated. NEK7 down-regulation attenuated neurological deficits, NLRP3 inflammasomes, caspase-1 activation, and neuronal injury. The same phenomena were observed during the in vitro experiments. Furthermore, NEK7 knock-down suppressed NLRP3 inflammasome activation and pyroptosis, which were triggered by K+ efflux, and the LPS + ATP-triggered NEK7–NLRP3 complex was reversed in primary cortical neurons placed in 50 mM K+ medium. Collectively, the data demonstrated that NEK7, as a modulator, regulates NLRP3 inflammasomes and downstream neuroinflammation in response to K+ efflux, through NEK7–NLRP3 assembly, pro-caspase-1 recruitment, caspase-1 activation, and pyroptosis in nerve injuries, post-TBI. NEK7 may be a potential therapeutic target for attenuating neuroinflammation and nerve injury post-TBI. |
| first_indexed | 2024-12-10T15:34:36Z |
| format | Article |
| id | doaj.art-b9f722ff602d476c8e3373f0a4e14a05 |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-12-10T15:34:36Z |
| publishDate | 2019-08-01 |
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| spelling | doaj.art-b9f722ff602d476c8e3373f0a4e14a052022-12-22T01:43:17ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992019-08-011210.3389/fnmol.2019.00202473797NEK7 Regulates NLRP3 Inflammasome Activation and Neuroinflammation Post-traumatic Brain InjuryYuhua Chen0Yuhua Chen1Jiao Meng2Jiao Meng3Fangfang Bi4Fangfang Bi5Hua Li6Cuicui Chang7Chen Ji8Wei Liu9Department of Central Laboratory, Xi’an Peihua University, Xi’an, ChinaDepartment of Neurosurgery, the Second Affiliated Hospital of Xi’an Medical University, Xi’an, ChinaDepartment of Central Laboratory, Xi’an Peihua University, Xi’an, ChinaDepartment of Basic Medical Science Research Center, Shaanxi Fourth People’s Hospital, Xi’an, ChinaDepartment of Central Laboratory, Xi’an Peihua University, Xi’an, ChinaDepartment of Basic Medical Science Research Center, Shaanxi Fourth People’s Hospital, Xi’an, ChinaDepartment of Basic Medical Science Research Center, Shaanxi Fourth People’s Hospital, Xi’an, ChinaDepartment of Central Laboratory, Xi’an Peihua University, Xi’an, ChinaDepartment of Central Laboratory, Xi’an Peihua University, Xi’an, ChinaDepartment of Neurosurgery, the Second Affiliated Hospital of Xi’an Medical University, Xi’an, ChinaAs one of the most common causes of mortality and disability, traumatic brain injury (TBI) is a huge psychological and economic burden to patients, families, and societies worldwide. Neuroinflammation reduction may be a favorable option to alleviate secondary brain injuries and ameliorate the outcome of TBI. The nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain-containing 3 (NLRP3) inflammasome, has been shown to be involved in TBI. NIMA-related kinase 7 (NEK7) has been verified as an essential mediator of NLRP3 inflammasome activation that is recruited upstream of the formation of inflammasomes in response to NLRP3 activators. However, the underlying mechanism by which NEK7 operates post-TBI remains undefined. In this study, we performed both in vivo and in vitro experiments. Using an in vivo mouse TBI model, mice were administered an intracerebroventricular injection of NEK7-shRNA virus. For the in vitro analysis, primary cortical neurons with NEK7-shRNA were stimulated with lipopolysaccharide (LPS)/ATP or potassium (K+). We evaluated the effects of NEK7 knock-down on neurological deficits, NLRP3 inflammasomes, caspase-1 activation, and neuronal injury. During the 0–168 h post-TBI period in vivo, NEK7 and NLRP3 inflammasome activation increased in what appeared to be a time-dependent manner. As well as pyroptosis-related markers, caspase-1 activation (p20) and interleukin-1β (IL-1β) activation (p17) were up-regulated. NEK7 down-regulation attenuated neurological deficits, NLRP3 inflammasomes, caspase-1 activation, and neuronal injury. The same phenomena were observed during the in vitro experiments. Furthermore, NEK7 knock-down suppressed NLRP3 inflammasome activation and pyroptosis, which were triggered by K+ efflux, and the LPS + ATP-triggered NEK7–NLRP3 complex was reversed in primary cortical neurons placed in 50 mM K+ medium. Collectively, the data demonstrated that NEK7, as a modulator, regulates NLRP3 inflammasomes and downstream neuroinflammation in response to K+ efflux, through NEK7–NLRP3 assembly, pro-caspase-1 recruitment, caspase-1 activation, and pyroptosis in nerve injuries, post-TBI. NEK7 may be a potential therapeutic target for attenuating neuroinflammation and nerve injury post-TBI.https://www.frontiersin.org/article/10.3389/fnmol.2019.00202/fulltraumatic brain injuryNLRP3 inflammasomeNEK7caspase-1 activationpyroptosispotassium efflux |
| spellingShingle | Yuhua Chen Yuhua Chen Jiao Meng Jiao Meng Fangfang Bi Fangfang Bi Hua Li Cuicui Chang Chen Ji Wei Liu NEK7 Regulates NLRP3 Inflammasome Activation and Neuroinflammation Post-traumatic Brain Injury Frontiers in Molecular Neuroscience traumatic brain injury NLRP3 inflammasome NEK7 caspase-1 activation pyroptosis potassium efflux |
| title | NEK7 Regulates NLRP3 Inflammasome Activation and Neuroinflammation Post-traumatic Brain Injury |
| title_full | NEK7 Regulates NLRP3 Inflammasome Activation and Neuroinflammation Post-traumatic Brain Injury |
| title_fullStr | NEK7 Regulates NLRP3 Inflammasome Activation and Neuroinflammation Post-traumatic Brain Injury |
| title_full_unstemmed | NEK7 Regulates NLRP3 Inflammasome Activation and Neuroinflammation Post-traumatic Brain Injury |
| title_short | NEK7 Regulates NLRP3 Inflammasome Activation and Neuroinflammation Post-traumatic Brain Injury |
| title_sort | nek7 regulates nlrp3 inflammasome activation and neuroinflammation post traumatic brain injury |
| topic | traumatic brain injury NLRP3 inflammasome NEK7 caspase-1 activation pyroptosis potassium efflux |
| url | https://www.frontiersin.org/article/10.3389/fnmol.2019.00202/full |
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