Spliceosomal Protein Gene <i>BmSPX</i> Regulates Reproductive Organ Development in <i>Bombyx mori</i>
Sex determination and differentiation are nearly universal to all eukaryotic organisms, encompassing diverse systems and mechanisms. Here, we identified a spliceosomal protein gene <i>BmSPX</i> involved in sex determination of the lepidopeteran insect, <i>Bombyx mori</i>. In...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-04-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/21/7/2579 |
| Summary: | Sex determination and differentiation are nearly universal to all eukaryotic organisms, encompassing diverse systems and mechanisms. Here, we identified a spliceosomal protein gene <i>BmSPX</i> involved in sex determination of the lepidopeteran insect, <i>Bombyx mori</i>. In a transgenic silkworm line that overexpressed the <i>BmSPX</i> gene, transgenic silkworm males exhibited differences in their external genitalia compared to wild-type males, but normal internal genitalia. Additionally, transgenic silkworm females exhibited a developmental disorder of the reproductive organs. Upregulation of <i>BmSPX</i> significantly increased the expression levels of sex-determining genes (<i>BmMasc</i> and <i>BmIMP</i>) and reduced the female-type splice isoform of <i>Bmdsx</i>, which is a key switch gene downstream of the sex-determination pathway. Additionally, co-immunoprecipitation assays confirmed an interaction between the BmSPX protein and BmPSI, an upstream regulatory factor of <i>Bmdsx</i>. Quantitative real-time PCR showed that BmSPX over-expression upregulated the expression of the <i>Hox</i> gene <i>abdominal-B</i> (<i>Adb-B</i>), which is required for specification of the posterior abdomen, external genitalia, and gonads of insects, as well as the genes in the Receptor Tyrosine Kinase (RTK) signaling pathway. In conclusion, our study suggested the involvement of BmSPX, identified as a novel regulatory factor, in the sex-determination pathway and regulation of reproductive organ development in silkworms. |
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| ISSN: | 1661-6596 1422-0067 |