Whole Genome Sequence of the Heterozygous Clinical Isolate Candida krusei 81-B-5
Candida krusei is a diploid, heterozygous yeast that is an opportunistic fungal pathogen in immunocompromised patients. This species also is utilized for fermenting cocoa beans during chocolate production. One major concern in the clinical setting is the innate resistance of this species to the most...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2017-09-01
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| Series: | G3: Genes, Genomes, Genetics |
| Subjects: | |
| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.117.043547 |
| _version_ | 1818890087128104960 |
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| author | Christina A. Cuomo Terrance Shea Bo Yang Reeta Rao Anja Forche |
| author_facet | Christina A. Cuomo Terrance Shea Bo Yang Reeta Rao Anja Forche |
| author_sort | Christina A. Cuomo |
| collection | DOAJ |
| description | Candida krusei is a diploid, heterozygous yeast that is an opportunistic fungal pathogen in immunocompromised patients. This species also is utilized for fermenting cocoa beans during chocolate production. One major concern in the clinical setting is the innate resistance of this species to the most commonly used antifungal drug fluconazole. Here, we report a high-quality genome sequence and assembly for the first clinical isolate of C. krusei, strain 81-B-5, into 11 scaffolds generated with PacBio sequencing technology. Gene annotation and comparative analysis revealed a unique profile of transporters that could play a role in drug resistance or adaptation to different environments. In addition, we show that, while 82% of the genome is highly heterozygous, a 2.0 Mb region of the largest scaffold has undergone loss of heterozygosity. This genome will serve as a reference for further genetic studies of this pathogen. |
| first_indexed | 2024-12-19T17:19:20Z |
| format | Article |
| id | doaj.art-b9f8280ffa2c4aabbe2af9335d876f56 |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-19T17:19:20Z |
| publishDate | 2017-09-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-b9f8280ffa2c4aabbe2af9335d876f562022-12-21T20:12:45ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362017-09-01792883288910.1534/g3.117.0435471Whole Genome Sequence of the Heterozygous Clinical Isolate Candida krusei 81-B-5Christina A. CuomoTerrance SheaBo YangReeta RaoAnja ForcheCandida krusei is a diploid, heterozygous yeast that is an opportunistic fungal pathogen in immunocompromised patients. This species also is utilized for fermenting cocoa beans during chocolate production. One major concern in the clinical setting is the innate resistance of this species to the most commonly used antifungal drug fluconazole. Here, we report a high-quality genome sequence and assembly for the first clinical isolate of C. krusei, strain 81-B-5, into 11 scaffolds generated with PacBio sequencing technology. Gene annotation and comparative analysis revealed a unique profile of transporters that could play a role in drug resistance or adaptation to different environments. In addition, we show that, while 82% of the genome is highly heterozygous, a 2.0 Mb region of the largest scaffold has undergone loss of heterozygosity. This genome will serve as a reference for further genetic studies of this pathogen.http://g3journal.org/lookup/doi/10.1534/g3.117.043547Candida krusei81-B-5heterozygosityLOHmating type locustransportersGenome Report |
| spellingShingle | Christina A. Cuomo Terrance Shea Bo Yang Reeta Rao Anja Forche Whole Genome Sequence of the Heterozygous Clinical Isolate Candida krusei 81-B-5 G3: Genes, Genomes, Genetics Candida krusei 81-B-5 heterozygosity LOH mating type locus transporters Genome Report |
| title | Whole Genome Sequence of the Heterozygous Clinical Isolate Candida krusei 81-B-5 |
| title_full | Whole Genome Sequence of the Heterozygous Clinical Isolate Candida krusei 81-B-5 |
| title_fullStr | Whole Genome Sequence of the Heterozygous Clinical Isolate Candida krusei 81-B-5 |
| title_full_unstemmed | Whole Genome Sequence of the Heterozygous Clinical Isolate Candida krusei 81-B-5 |
| title_short | Whole Genome Sequence of the Heterozygous Clinical Isolate Candida krusei 81-B-5 |
| title_sort | whole genome sequence of the heterozygous clinical isolate candida krusei 81 b 5 |
| topic | Candida krusei 81-B-5 heterozygosity LOH mating type locus transporters Genome Report |
| url | http://g3journal.org/lookup/doi/10.1534/g3.117.043547 |
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