Eu-Chelate Polystyrene Microsphere-Based Lateral Flow Immunoassay Platform for hs-CRP Detection
<b>Inflammation</b> caused by viral or bacterial infection is a major threat to human health globally. Blood C-reactive protein (CRP) has been proven to be a sensitive indicator for the occurrence and development of inflammation. Furthermore, a tiny change of blood CRP concentration may...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/11/977 |
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| author | Birui Jin Zhiguo Du Chuyao Zhang Zhao Yu Xuemin Wang Jie Hu Zedong Li |
| author_facet | Birui Jin Zhiguo Du Chuyao Zhang Zhao Yu Xuemin Wang Jie Hu Zedong Li |
| author_sort | Birui Jin |
| collection | DOAJ |
| description | <b>Inflammation</b> caused by viral or bacterial infection is a major threat to human health globally. Blood C-reactive protein (CRP) has been proven to be a sensitive indicator for the occurrence and development of inflammation. Furthermore, a tiny change of blood CRP concentration may portend chronic diseases; therefore, high-sensitivity CRP (hs-CRP) detection in a quantitative, rapid, user-friendly, and low-cost manner is highly demanded. In this paper, we developed a europium-chelate polystyrene microsphere (EuPSM)-based lateral flow immunoassay (LFIA) integrating with a benchtop fluorescence analyzer for hs-CRP detection. The optimization of the EuPSM-based LFIA was implemented through adjusting the antibody density on EuPSM from 100% to 60% of the saturated density. Finally, the limit of detection of 0.76 pg/mL and detection range of 0.025–250 ng/mL were obtained. Moreover, the clinical application capability of the proposed platform was validated through detecting CRP in clinical serum samples, showing high consistency with the results obtained from the clinical standard method. Hence, the proposed EuPSM-based LFIA has been verified to be well suitable for hs-CRP detection, while also showing great applicability for sensitively and rapidly detecting other biomarkers. |
| first_indexed | 2024-03-09T19:14:31Z |
| format | Article |
| id | doaj.art-b9fa05c9d1994b95b38b52daa96da13b |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T19:14:31Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-b9fa05c9d1994b95b38b52daa96da13b2023-11-24T03:55:18ZengMDPI AGBiosensors2079-63742022-11-01121197710.3390/bios12110977Eu-Chelate Polystyrene Microsphere-Based Lateral Flow Immunoassay Platform for hs-CRP DetectionBirui Jin0Zhiguo Du1Chuyao Zhang2Zhao Yu3Xuemin Wang4Jie Hu5Zedong Li6School of Materials and Chemical Engineering, Xi’an Technological University, Xi’an 710021, ChinaSchool of Materials and Chemical Engineering, Xi’an Technological University, Xi’an 710021, ChinaSchool of Materials and Chemical Engineering, Xi’an Technological University, Xi’an 710021, ChinaXi’an Thermal Power Research Institute Co., Ltd., Xi’an 710054, ChinaThe Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, ChinaSuzhou DiYinAn Biotech Co., Ltd., Suzhou Innovation Center for Life Science and Technology, Suzhou 215129, ChinaBioinspired Engineering and Biomechanics Center (BEBC), Xi’an Jiaotong University, Xi’an 710049, China<b>Inflammation</b> caused by viral or bacterial infection is a major threat to human health globally. Blood C-reactive protein (CRP) has been proven to be a sensitive indicator for the occurrence and development of inflammation. Furthermore, a tiny change of blood CRP concentration may portend chronic diseases; therefore, high-sensitivity CRP (hs-CRP) detection in a quantitative, rapid, user-friendly, and low-cost manner is highly demanded. In this paper, we developed a europium-chelate polystyrene microsphere (EuPSM)-based lateral flow immunoassay (LFIA) integrating with a benchtop fluorescence analyzer for hs-CRP detection. The optimization of the EuPSM-based LFIA was implemented through adjusting the antibody density on EuPSM from 100% to 60% of the saturated density. Finally, the limit of detection of 0.76 pg/mL and detection range of 0.025–250 ng/mL were obtained. Moreover, the clinical application capability of the proposed platform was validated through detecting CRP in clinical serum samples, showing high consistency with the results obtained from the clinical standard method. Hence, the proposed EuPSM-based LFIA has been verified to be well suitable for hs-CRP detection, while also showing great applicability for sensitively and rapidly detecting other biomarkers.https://www.mdpi.com/2079-6374/12/11/977fluorescence analysissensitivity enhancementpaper microfluidicshs-CRP detection |
| spellingShingle | Birui Jin Zhiguo Du Chuyao Zhang Zhao Yu Xuemin Wang Jie Hu Zedong Li Eu-Chelate Polystyrene Microsphere-Based Lateral Flow Immunoassay Platform for hs-CRP Detection Biosensors fluorescence analysis sensitivity enhancement paper microfluidics hs-CRP detection |
| title | Eu-Chelate Polystyrene Microsphere-Based Lateral Flow Immunoassay Platform for hs-CRP Detection |
| title_full | Eu-Chelate Polystyrene Microsphere-Based Lateral Flow Immunoassay Platform for hs-CRP Detection |
| title_fullStr | Eu-Chelate Polystyrene Microsphere-Based Lateral Flow Immunoassay Platform for hs-CRP Detection |
| title_full_unstemmed | Eu-Chelate Polystyrene Microsphere-Based Lateral Flow Immunoassay Platform for hs-CRP Detection |
| title_short | Eu-Chelate Polystyrene Microsphere-Based Lateral Flow Immunoassay Platform for hs-CRP Detection |
| title_sort | eu chelate polystyrene microsphere based lateral flow immunoassay platform for hs crp detection |
| topic | fluorescence analysis sensitivity enhancement paper microfluidics hs-CRP detection |
| url | https://www.mdpi.com/2079-6374/12/11/977 |
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