H2O2 biosensor consisted of hemoglobin-DNA conjugate on nanoporous gold thin film electrode with electrochemical signal enhancement
Abstract In this research, we developed electrochemical biosensor which was composed of hemoglobin (Hb)-DNA conjugate on nanoporous gold thin film (NPGF) for hydrogen peroxide (H2O2) detection. For the first time, Hb and DNA was conjugated as a sensing platform for uniform orientation of Hb on elect...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-01-01
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| Series: | Nano Convergence |
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| Online Access: | http://link.springer.com/article/10.1186/s40580-018-0172-z |
| _version_ | 1818024946272567296 |
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| author | Jinhee Jo Jinho Yoon Taek Lee Hyeon-Yeol Cho Ji-Young Lee Jeong-Woo Choi |
| author_facet | Jinhee Jo Jinho Yoon Taek Lee Hyeon-Yeol Cho Ji-Young Lee Jeong-Woo Choi |
| author_sort | Jinhee Jo |
| collection | DOAJ |
| description | Abstract In this research, we developed electrochemical biosensor which was composed of hemoglobin (Hb)-DNA conjugate on nanoporous gold thin film (NPGF) for hydrogen peroxide (H2O2) detection. For the first time, Hb and DNA was conjugated as a sensing platform for uniform orientation of Hb on electrode. The newly developed Hb-DNA conjugate was designed to prevent Hb from aggregation on electrode. DNA hybridization of Hb-DNA conjugate and complementary DNA (cDNA) on NPGF electrode induced uniformly assembled biosensor. Furthermore, NPGF electrode fabrication method was introduced to the increment of the surface area. To confirm the conjugation of Hb-DNA conjugate, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and ultraviolet–visible spectroscopy (UV–VIS) were used. Formation of the NPGF electrode was verified by scanning electron microscope (SEM). Atomic force microscopy (AFM) was operated for the confirmation of Hb-DNA immobilization on electrode. The electrochemical property of fabricated electrode was investigated by cyclic voltammetry (CV). Also, H2O2 sensing performance of fabricated electrode was investigated by amperometric i-t curve technique. This sensor showed a wide linear range from 0.00025 to 5.00 mM and a correlation coefficient of R2 = 0.9986. The detection limit was 250 nM. Proposed biosensor can be utilized as a sensing platform for development of biosensor. |
| first_indexed | 2024-12-10T04:08:17Z |
| format | Article |
| id | doaj.art-b9fc02ca10384631b5ca0de7472450fd |
| institution | Directory Open Access Journal |
| issn | 2196-5404 |
| language | English |
| last_indexed | 2024-12-10T04:08:17Z |
| publishDate | 2019-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nano Convergence |
| spelling | doaj.art-b9fc02ca10384631b5ca0de7472450fd2022-12-22T02:02:47ZengSpringerOpenNano Convergence2196-54042019-01-01611810.1186/s40580-018-0172-zH2O2 biosensor consisted of hemoglobin-DNA conjugate on nanoporous gold thin film electrode with electrochemical signal enhancementJinhee Jo0Jinho Yoon1Taek Lee2Hyeon-Yeol Cho3Ji-Young Lee4Jeong-Woo Choi5Department of Chemical and Biomolecular Engineering, Sogang UniversityDepartment of Chemical and Biomolecular Engineering, Sogang UniversityDepartment of Chemical and Biomolecular Engineering, Sogang UniversityDepartment of Chemical and Biomolecular Engineering, Sogang UniversityDepartment of Chemical and Biomolecular Engineering, Sogang UniversityDepartment of Chemical and Biomolecular Engineering, Sogang UniversityAbstract In this research, we developed electrochemical biosensor which was composed of hemoglobin (Hb)-DNA conjugate on nanoporous gold thin film (NPGF) for hydrogen peroxide (H2O2) detection. For the first time, Hb and DNA was conjugated as a sensing platform for uniform orientation of Hb on electrode. The newly developed Hb-DNA conjugate was designed to prevent Hb from aggregation on electrode. DNA hybridization of Hb-DNA conjugate and complementary DNA (cDNA) on NPGF electrode induced uniformly assembled biosensor. Furthermore, NPGF electrode fabrication method was introduced to the increment of the surface area. To confirm the conjugation of Hb-DNA conjugate, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and ultraviolet–visible spectroscopy (UV–VIS) were used. Formation of the NPGF electrode was verified by scanning electron microscope (SEM). Atomic force microscopy (AFM) was operated for the confirmation of Hb-DNA immobilization on electrode. The electrochemical property of fabricated electrode was investigated by cyclic voltammetry (CV). Also, H2O2 sensing performance of fabricated electrode was investigated by amperometric i-t curve technique. This sensor showed a wide linear range from 0.00025 to 5.00 mM and a correlation coefficient of R2 = 0.9986. The detection limit was 250 nM. Proposed biosensor can be utilized as a sensing platform for development of biosensor.http://link.springer.com/article/10.1186/s40580-018-0172-zBiosensorDNA hybridizationHemoglobinHydrogen peroxideNanoporous gold thin film |
| spellingShingle | Jinhee Jo Jinho Yoon Taek Lee Hyeon-Yeol Cho Ji-Young Lee Jeong-Woo Choi H2O2 biosensor consisted of hemoglobin-DNA conjugate on nanoporous gold thin film electrode with electrochemical signal enhancement Nano Convergence Biosensor DNA hybridization Hemoglobin Hydrogen peroxide Nanoporous gold thin film |
| title | H2O2 biosensor consisted of hemoglobin-DNA conjugate on nanoporous gold thin film electrode with electrochemical signal enhancement |
| title_full | H2O2 biosensor consisted of hemoglobin-DNA conjugate on nanoporous gold thin film electrode with electrochemical signal enhancement |
| title_fullStr | H2O2 biosensor consisted of hemoglobin-DNA conjugate on nanoporous gold thin film electrode with electrochemical signal enhancement |
| title_full_unstemmed | H2O2 biosensor consisted of hemoglobin-DNA conjugate on nanoporous gold thin film electrode with electrochemical signal enhancement |
| title_short | H2O2 biosensor consisted of hemoglobin-DNA conjugate on nanoporous gold thin film electrode with electrochemical signal enhancement |
| title_sort | h2o2 biosensor consisted of hemoglobin dna conjugate on nanoporous gold thin film electrode with electrochemical signal enhancement |
| topic | Biosensor DNA hybridization Hemoglobin Hydrogen peroxide Nanoporous gold thin film |
| url | http://link.springer.com/article/10.1186/s40580-018-0172-z |
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