Oxidative RNA Modifications as an Early Response of Soybean (Glycine max L.) Exposed to Copper and Lead
Plant exposure to metals is associated with the accumulation of reactive oxygen species, which mediate the oxidation of various molecules including lipids, proteins, and nucleic acids. The aim of the present study is the evaluation of the impact of short-term Cu and Pb treatment on oxidative events...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.828620/full |
| _version_ | 1830176698501431296 |
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| author | Jagna Chmielowska-Bąk Ekaterina Shcheglova Konrad Rosik Nikita Yushin Inga Zinicovscaia Inga Zinicovscaia Joanna Deckert |
| author_facet | Jagna Chmielowska-Bąk Ekaterina Shcheglova Konrad Rosik Nikita Yushin Inga Zinicovscaia Inga Zinicovscaia Joanna Deckert |
| author_sort | Jagna Chmielowska-Bąk |
| collection | DOAJ |
| description | Plant exposure to metals is associated with the accumulation of reactive oxygen species, which mediate the oxidation of various molecules including lipids, proteins, and nucleic acids. The aim of the present study is the evaluation of the impact of short-term Cu and Pb treatment on oxidative events in the roots of soybean seedlings, with special emphasis on RNA oxidation. The results show that an increase in total RNA oxidative modification, 8-hydroxyguanosine (8-OHG), constitutes a very early response to both applied metals, observed already within the first hour of treatment. Exposure to Cu and Pb resulted also in the increase in superoxide anion and hydrogen peroxide levels and intensified lipid peroxidation. However, these responses were most prominent after longer treatment times. On the other hand, no changes were observed in the level of protein carbonylation. It can be concluded that 8-OHG enrichment in total RNA constitutes one of the earliest reactions to metals, which precedes the symptoms of oxidative stress. |
| first_indexed | 2024-12-17T19:11:20Z |
| format | Article |
| id | doaj.art-ba01836c26214664825692d26a306af4 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-17T19:11:20Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-ba01836c26214664825692d26a306af42022-12-21T21:35:52ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-01-011210.3389/fpls.2021.828620828620Oxidative RNA Modifications as an Early Response of Soybean (Glycine max L.) Exposed to Copper and LeadJagna Chmielowska-Bąk0Ekaterina Shcheglova1Konrad Rosik2Nikita Yushin3Inga Zinicovscaia4Inga Zinicovscaia5Joanna Deckert6Department of Plant Ecophysiology, Faculty of Biology, Institute of Experimental Biology, Adam Mickiewicz University, Poznań, PolandDepartment of Plant Ecophysiology, Faculty of Biology, Institute of Experimental Biology, Adam Mickiewicz University, Poznań, PolandDepartment of Plant Ecophysiology, Faculty of Biology, Institute of Experimental Biology, Adam Mickiewicz University, Poznań, PolandFrank Laboratory of Neutron Physics, Joint Institute for Nuclear Research, Dubna, RussiaFrank Laboratory of Neutron Physics, Joint Institute for Nuclear Research, Dubna, RussiaHoria Hulubei National Institute for R&D in Physics and Nuclear Engineering, Magurele, RomaniaDepartment of Plant Ecophysiology, Faculty of Biology, Institute of Experimental Biology, Adam Mickiewicz University, Poznań, PolandPlant exposure to metals is associated with the accumulation of reactive oxygen species, which mediate the oxidation of various molecules including lipids, proteins, and nucleic acids. The aim of the present study is the evaluation of the impact of short-term Cu and Pb treatment on oxidative events in the roots of soybean seedlings, with special emphasis on RNA oxidation. The results show that an increase in total RNA oxidative modification, 8-hydroxyguanosine (8-OHG), constitutes a very early response to both applied metals, observed already within the first hour of treatment. Exposure to Cu and Pb resulted also in the increase in superoxide anion and hydrogen peroxide levels and intensified lipid peroxidation. However, these responses were most prominent after longer treatment times. On the other hand, no changes were observed in the level of protein carbonylation. It can be concluded that 8-OHG enrichment in total RNA constitutes one of the earliest reactions to metals, which precedes the symptoms of oxidative stress.https://www.frontiersin.org/articles/10.3389/fpls.2021.828620/full8-hydroxyguanosine8-OHGRNA modificationsoxidative stressROSmetal stress |
| spellingShingle | Jagna Chmielowska-Bąk Ekaterina Shcheglova Konrad Rosik Nikita Yushin Inga Zinicovscaia Inga Zinicovscaia Joanna Deckert Oxidative RNA Modifications as an Early Response of Soybean (Glycine max L.) Exposed to Copper and Lead Frontiers in Plant Science 8-hydroxyguanosine 8-OHG RNA modifications oxidative stress ROS metal stress |
| title | Oxidative RNA Modifications as an Early Response of Soybean (Glycine max L.) Exposed to Copper and Lead |
| title_full | Oxidative RNA Modifications as an Early Response of Soybean (Glycine max L.) Exposed to Copper and Lead |
| title_fullStr | Oxidative RNA Modifications as an Early Response of Soybean (Glycine max L.) Exposed to Copper and Lead |
| title_full_unstemmed | Oxidative RNA Modifications as an Early Response of Soybean (Glycine max L.) Exposed to Copper and Lead |
| title_short | Oxidative RNA Modifications as an Early Response of Soybean (Glycine max L.) Exposed to Copper and Lead |
| title_sort | oxidative rna modifications as an early response of soybean glycine max l exposed to copper and lead |
| topic | 8-hydroxyguanosine 8-OHG RNA modifications oxidative stress ROS metal stress |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.828620/full |
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