Diverse Bacteriophages Infecting the Bacterial Striped Catfish Pathogen <i>Edwardsiella ictaluri</i>
Bacteriophages infecting <i>Edwardsiella ictaluri</i> have been less investigated, although the host bacterium is one of the most important fish pathogens causing enteric septicemia of catfish (ESC). We present here two distinctly novel bacteriophages vB_EiM_PVN06 and vB_EiA_PVN09 infect...
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MDPI AG
2021-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/9/1830 |
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| author | Tan-Trung Nguyen Tran T. T. Xuan To H. Ngoc Le T. My Duyen Tu Q. Vinh Pham D. T. My Hoang A. Hoang Le P. Nga |
| author_facet | Tan-Trung Nguyen Tran T. T. Xuan To H. Ngoc Le T. My Duyen Tu Q. Vinh Pham D. T. My Hoang A. Hoang Le P. Nga |
| author_sort | Tan-Trung Nguyen |
| collection | DOAJ |
| description | Bacteriophages infecting <i>Edwardsiella ictaluri</i> have been less investigated, although the host bacterium is one of the most important fish pathogens causing enteric septicemia of catfish (ESC). We present here two distinctly novel bacteriophages vB_EiM_PVN06 and vB_EiA_PVN09 infecting <i>Edwardsiella ictaluri</i> E1, with their geographical origins from the Mekong Delta, Vietnam. Bacteriophage vB_EiM_PVN06 native to a mud sample reveals complete differences of biological properties with the phage vB_EiA_PVN09 originated from a viscus of a healthy catfish (<i>Pangasianodon hypophthalmus</i>) cultured in the same area. Morphological analyses combined with genomic data indicate that phage vB_EiM_PVN06 is classified to <i>Myoviridae</i> family and shares high similarity with <i>E. ictaluri</i> phage PEi21 genome, while vB_EiA_PVN09 is a member of <i>Teseptimavirus</i> genus, <i>Autographiviridae</i> family, and mostly closes to phage vB_EcoP_IME390. The vB_EiA_PVN09 is a T7-like bacteriophage, which has been firstly found infecting to <i>E. ictaluri</i>, and host range analysis also evidences for the cross-infection of this phage to <i>Escherichia coli</i> K12 and <i>Escherichia coli</i> DH5α. Together, our research highlights the diversity of bacteriophages infecting the pathogen <i>E. ictaluri</i> and suggests further explorations of lytic phages in environmental niches, to be exploited in feasible strategies of phage therapy in ESC disease control. |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T07:26:24Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj.art-ba01cefb88864831a9c70b2b522ebb0a2023-11-22T14:17:45ZengMDPI AGMicroorganisms2076-26072021-08-0199183010.3390/microorganisms9091830Diverse Bacteriophages Infecting the Bacterial Striped Catfish Pathogen <i>Edwardsiella ictaluri</i>Tan-Trung Nguyen0Tran T. T. Xuan1To H. Ngoc2Le T. My Duyen3Tu Q. Vinh4Pham D. T. My5Hoang A. Hoang6Le P. Nga7Faculty of Chemical Engineering, Ho Chi Minh City University of Technology (HCMUT), 268 Ly Thuong Kiet, District 10, Ho Chi Minh City 700000, VietnamFaculty of Chemical Engineering, Ho Chi Minh City University of Technology (HCMUT), 268 Ly Thuong Kiet, District 10, Ho Chi Minh City 700000, VietnamFaculty of Chemical Engineering, Ho Chi Minh City University of Technology (HCMUT), 268 Ly Thuong Kiet, District 10, Ho Chi Minh City 700000, VietnamFaculty of Chemical Engineering, Ho Chi Minh City University of Technology (HCMUT), 268 Ly Thuong Kiet, District 10, Ho Chi Minh City 700000, VietnamFaculty of Chemical Engineering, Ho Chi Minh City University of Technology (HCMUT), 268 Ly Thuong Kiet, District 10, Ho Chi Minh City 700000, VietnamFaculty of Chemical Engineering, Ho Chi Minh City University of Technology (HCMUT), 268 Ly Thuong Kiet, District 10, Ho Chi Minh City 700000, VietnamFaculty of Chemical Engineering, Ho Chi Minh City University of Technology (HCMUT), 268 Ly Thuong Kiet, District 10, Ho Chi Minh City 700000, VietnamFaculty of Chemical Engineering, Ho Chi Minh City University of Technology (HCMUT), 268 Ly Thuong Kiet, District 10, Ho Chi Minh City 700000, VietnamBacteriophages infecting <i>Edwardsiella ictaluri</i> have been less investigated, although the host bacterium is one of the most important fish pathogens causing enteric septicemia of catfish (ESC). We present here two distinctly novel bacteriophages vB_EiM_PVN06 and vB_EiA_PVN09 infecting <i>Edwardsiella ictaluri</i> E1, with their geographical origins from the Mekong Delta, Vietnam. Bacteriophage vB_EiM_PVN06 native to a mud sample reveals complete differences of biological properties with the phage vB_EiA_PVN09 originated from a viscus of a healthy catfish (<i>Pangasianodon hypophthalmus</i>) cultured in the same area. Morphological analyses combined with genomic data indicate that phage vB_EiM_PVN06 is classified to <i>Myoviridae</i> family and shares high similarity with <i>E. ictaluri</i> phage PEi21 genome, while vB_EiA_PVN09 is a member of <i>Teseptimavirus</i> genus, <i>Autographiviridae</i> family, and mostly closes to phage vB_EcoP_IME390. The vB_EiA_PVN09 is a T7-like bacteriophage, which has been firstly found infecting to <i>E. ictaluri</i>, and host range analysis also evidences for the cross-infection of this phage to <i>Escherichia coli</i> K12 and <i>Escherichia coli</i> DH5α. Together, our research highlights the diversity of bacteriophages infecting the pathogen <i>E. ictaluri</i> and suggests further explorations of lytic phages in environmental niches, to be exploited in feasible strategies of phage therapy in ESC disease control.https://www.mdpi.com/2076-2607/9/9/1830bacteriophages<i>Edwardsiella ictaluri</i><i>Edwardsiella</i> phagegenome comparisongenome analysis<i>Autographiviridae</i> |
| spellingShingle | Tan-Trung Nguyen Tran T. T. Xuan To H. Ngoc Le T. My Duyen Tu Q. Vinh Pham D. T. My Hoang A. Hoang Le P. Nga Diverse Bacteriophages Infecting the Bacterial Striped Catfish Pathogen <i>Edwardsiella ictaluri</i> Microorganisms bacteriophages <i>Edwardsiella ictaluri</i> <i>Edwardsiella</i> phage genome comparison genome analysis <i>Autographiviridae</i> |
| title | Diverse Bacteriophages Infecting the Bacterial Striped Catfish Pathogen <i>Edwardsiella ictaluri</i> |
| title_full | Diverse Bacteriophages Infecting the Bacterial Striped Catfish Pathogen <i>Edwardsiella ictaluri</i> |
| title_fullStr | Diverse Bacteriophages Infecting the Bacterial Striped Catfish Pathogen <i>Edwardsiella ictaluri</i> |
| title_full_unstemmed | Diverse Bacteriophages Infecting the Bacterial Striped Catfish Pathogen <i>Edwardsiella ictaluri</i> |
| title_short | Diverse Bacteriophages Infecting the Bacterial Striped Catfish Pathogen <i>Edwardsiella ictaluri</i> |
| title_sort | diverse bacteriophages infecting the bacterial striped catfish pathogen i edwardsiella ictaluri i |
| topic | bacteriophages <i>Edwardsiella ictaluri</i> <i>Edwardsiella</i> phage genome comparison genome analysis <i>Autographiviridae</i> |
| url | https://www.mdpi.com/2076-2607/9/9/1830 |
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