Fabrication of Anisotropic Cu Ferrite-Polymer Core-Shell Nanoparticles for Photodynamic Ablation of Cervical Cancer Cells
In this work we developed methylene blue-immobilized copper-iron nanoparticles (MB-CuFe NPs) through a facile one-step hydrothermal reaction to achieve a better phototherapeutic effect. The Fe/Cu ratio of the CuFe NPs was controllable by merely changing the loading amount of iron precursor concentra...
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MDPI AG
2020-12-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/12/2429 |
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| author | Shuo-Hsiu Kuo Po-Ting Wu Jing-Yin Huang Chin-Pao Chiu Jiashing Yu Mei-Yi Liao |
| author_facet | Shuo-Hsiu Kuo Po-Ting Wu Jing-Yin Huang Chin-Pao Chiu Jiashing Yu Mei-Yi Liao |
| author_sort | Shuo-Hsiu Kuo |
| collection | DOAJ |
| description | In this work we developed methylene blue-immobilized copper-iron nanoparticles (MB-CuFe NPs) through a facile one-step hydrothermal reaction to achieve a better phototherapeutic effect. The Fe/Cu ratio of the CuFe NPs was controllable by merely changing the loading amount of iron precursor concentration. The CuFe NPs could serve as a Fenton catalyst to convert hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) into reactive oxygen species (ROS), while the superparamagnetic properties also suggest magnetic resonance imaging (MRI) potential. Furthermore, the Food and Drug Administration (FDA)-approved MB photosensitizer could strongly adsorb onto the surface of CuFe NPs to facilitate the drug delivery into cells and improve the photodynamic therapy at 660 nm via significant generation of singlet oxygen species, leading to enhanced cancer cell-damaging efficacy. An MTT (thiazolyl blue tetrazolium bromide) assay proved the low cytotoxicity of the CuFe NPs to cervical cancer cells (HeLa cells), namely above 80% at 25 ppm of the sample dose. A slight dissolution of Cu and Fe ions from the CuFe NPs in an acidic environment was obtained, providing direct evidence for CuFe NPs being degradable without the risk of long-term retention in the body. Moreover, the tremendous photo-to-thermal conversion of CuFe NPs was examined, which might be combined with photodynamic therapy (PDT) for promising development in the depletion of cancer cells after a single pulse of deep-red light irradiation at high laser power. |
| first_indexed | 2024-03-10T14:19:44Z |
| format | Article |
| id | doaj.art-ba03ada7aa814583a0762d0b607d8811 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T14:19:44Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-ba03ada7aa814583a0762d0b607d88112023-11-20T23:31:59ZengMDPI AGNanomaterials2079-49912020-12-011012242910.3390/nano10122429Fabrication of Anisotropic Cu Ferrite-Polymer Core-Shell Nanoparticles for Photodynamic Ablation of Cervical Cancer CellsShuo-Hsiu Kuo0Po-Ting Wu1Jing-Yin Huang2Chin-Pao Chiu3Jiashing Yu4Mei-Yi Liao5Department of Chemical Engineering, National Taiwan University, Taipei 10617, TaiwanDepartment of Chemical Engineering, National Taiwan University, Taipei 10617, TaiwanDepartment of Applied Chemistry, National Pingtung University, Pingtung 90003, TaiwanDepartment of Applied Chemistry, National Pingtung University, Pingtung 90003, TaiwanDepartment of Chemical Engineering, National Taiwan University, Taipei 10617, TaiwanDepartment of Applied Chemistry, National Pingtung University, Pingtung 90003, TaiwanIn this work we developed methylene blue-immobilized copper-iron nanoparticles (MB-CuFe NPs) through a facile one-step hydrothermal reaction to achieve a better phototherapeutic effect. The Fe/Cu ratio of the CuFe NPs was controllable by merely changing the loading amount of iron precursor concentration. The CuFe NPs could serve as a Fenton catalyst to convert hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) into reactive oxygen species (ROS), while the superparamagnetic properties also suggest magnetic resonance imaging (MRI) potential. Furthermore, the Food and Drug Administration (FDA)-approved MB photosensitizer could strongly adsorb onto the surface of CuFe NPs to facilitate the drug delivery into cells and improve the photodynamic therapy at 660 nm via significant generation of singlet oxygen species, leading to enhanced cancer cell-damaging efficacy. An MTT (thiazolyl blue tetrazolium bromide) assay proved the low cytotoxicity of the CuFe NPs to cervical cancer cells (HeLa cells), namely above 80% at 25 ppm of the sample dose. A slight dissolution of Cu and Fe ions from the CuFe NPs in an acidic environment was obtained, providing direct evidence for CuFe NPs being degradable without the risk of long-term retention in the body. Moreover, the tremendous photo-to-thermal conversion of CuFe NPs was examined, which might be combined with photodynamic therapy (PDT) for promising development in the depletion of cancer cells after a single pulse of deep-red light irradiation at high laser power.https://www.mdpi.com/2079-4991/10/12/2429bimetallic nanoparticlessuperparamagnetic nanoparticlesFenton reactionreactive oxygen speciescancer treatmentphotodynamic therapy |
| spellingShingle | Shuo-Hsiu Kuo Po-Ting Wu Jing-Yin Huang Chin-Pao Chiu Jiashing Yu Mei-Yi Liao Fabrication of Anisotropic Cu Ferrite-Polymer Core-Shell Nanoparticles for Photodynamic Ablation of Cervical Cancer Cells Nanomaterials bimetallic nanoparticles superparamagnetic nanoparticles Fenton reaction reactive oxygen species cancer treatment photodynamic therapy |
| title | Fabrication of Anisotropic Cu Ferrite-Polymer Core-Shell Nanoparticles for Photodynamic Ablation of Cervical Cancer Cells |
| title_full | Fabrication of Anisotropic Cu Ferrite-Polymer Core-Shell Nanoparticles for Photodynamic Ablation of Cervical Cancer Cells |
| title_fullStr | Fabrication of Anisotropic Cu Ferrite-Polymer Core-Shell Nanoparticles for Photodynamic Ablation of Cervical Cancer Cells |
| title_full_unstemmed | Fabrication of Anisotropic Cu Ferrite-Polymer Core-Shell Nanoparticles for Photodynamic Ablation of Cervical Cancer Cells |
| title_short | Fabrication of Anisotropic Cu Ferrite-Polymer Core-Shell Nanoparticles for Photodynamic Ablation of Cervical Cancer Cells |
| title_sort | fabrication of anisotropic cu ferrite polymer core shell nanoparticles for photodynamic ablation of cervical cancer cells |
| topic | bimetallic nanoparticles superparamagnetic nanoparticles Fenton reaction reactive oxygen species cancer treatment photodynamic therapy |
| url | https://www.mdpi.com/2079-4991/10/12/2429 |
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