SDG102, a H3K36-Methyltransferase-Encoding Gene, Plays Pleiotropic Roles in Growth and Development of Maize (<i>Zea mays</i> L.)
Although histone lysine methylation has been studied in thale cress (<i>Arabidopsis thaliana</i> (L.) Heynh.) and rice (<i>Oryza sativa</i> L.) in recent years, its function in maize (<i>Zea mays</i> L.) remains poorly characterized. To better understand the funct...
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MDPI AG
2022-07-01
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| Series: | International Journal of Molecular Sciences |
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| author | Yongjian Li Weifeng Sun Zhenhui Wang Chang Wan Jun Zhang Xin Qi Jian Zhang |
| author_facet | Yongjian Li Weifeng Sun Zhenhui Wang Chang Wan Jun Zhang Xin Qi Jian Zhang |
| author_sort | Yongjian Li |
| collection | DOAJ |
| description | Although histone lysine methylation has been studied in thale cress (<i>Arabidopsis thaliana</i> (L.) Heynh.) and rice (<i>Oryza sativa</i> L.) in recent years, its function in maize (<i>Zea mays</i> L.) remains poorly characterized. To better understand the function of histone lysine methylation in maize, SDG102, a H3 lysine 36 (H3K36) methylase, was chosen for functional characterization using overexpressed and knockout transgenic plants. SDG102-deficiency in maize caused multiple phenotypes including yellow leaves in seedlings, late-flowering, and increased adult plant height, while the overexpression of <i>SDG102</i> led to reduced adult plant height. The key flowering genes, <i>ZCN8/ZCN7</i> and <i>MADS4/MADA67</i>, were downregulated in SDG102-deficient plants. Chromatin immunoprecipitation (ChIP) experiments showed that H3 lysine 36 trimethylation (H3K36me3) levels were reduced at these loci. Perturbation of <i>SDG102</i> expression caused the misexpression of multiple genes. Interestingly, the overexpression or knockout of <i>SDG102</i> also led to genome-wide decreases and increases in the H3K36me3 levels, respectively. Together, our results suggest that SDG102 is a methyltransferase that catalyzes the trimethylation of H3K36 of many genes across the maize genome, which are involved in multiple biological processes including those controlling flowering time. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:46:50Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ba04cc22804a4de1bac927a9b73a2f0a2023-11-23T20:14:22ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-07-012313745810.3390/ijms23137458SDG102, a H3K36-Methyltransferase-Encoding Gene, Plays Pleiotropic Roles in Growth and Development of Maize (<i>Zea mays</i> L.)Yongjian Li0Weifeng Sun1Zhenhui Wang2Chang Wan3Jun Zhang4Xin Qi5Jian Zhang6Faculty of Agronomy, Jilin Agricultural University, Changchun 130118, ChinaFaculty of Agronomy, Jilin Agricultural University, Changchun 130118, ChinaFaculty of Agronomy, Jilin Agricultural University, Changchun 130118, ChinaFaculty of Agronomy, Jilin Agricultural University, Changchun 130118, ChinaFaculty of Agronomy, Jilin Agricultural University, Changchun 130118, ChinaFaculty of Agronomy, Jilin Agricultural University, Changchun 130118, ChinaFaculty of Agronomy, Jilin Agricultural University, Changchun 130118, ChinaAlthough histone lysine methylation has been studied in thale cress (<i>Arabidopsis thaliana</i> (L.) Heynh.) and rice (<i>Oryza sativa</i> L.) in recent years, its function in maize (<i>Zea mays</i> L.) remains poorly characterized. To better understand the function of histone lysine methylation in maize, SDG102, a H3 lysine 36 (H3K36) methylase, was chosen for functional characterization using overexpressed and knockout transgenic plants. SDG102-deficiency in maize caused multiple phenotypes including yellow leaves in seedlings, late-flowering, and increased adult plant height, while the overexpression of <i>SDG102</i> led to reduced adult plant height. The key flowering genes, <i>ZCN8/ZCN7</i> and <i>MADS4/MADA67</i>, were downregulated in SDG102-deficient plants. Chromatin immunoprecipitation (ChIP) experiments showed that H3 lysine 36 trimethylation (H3K36me3) levels were reduced at these loci. Perturbation of <i>SDG102</i> expression caused the misexpression of multiple genes. Interestingly, the overexpression or knockout of <i>SDG102</i> also led to genome-wide decreases and increases in the H3K36me3 levels, respectively. Together, our results suggest that SDG102 is a methyltransferase that catalyzes the trimethylation of H3K36 of many genes across the maize genome, which are involved in multiple biological processes including those controlling flowering time.https://www.mdpi.com/1422-0067/23/13/7458maizehistone methylationflowering timeH3K36SDG102 |
| spellingShingle | Yongjian Li Weifeng Sun Zhenhui Wang Chang Wan Jun Zhang Xin Qi Jian Zhang SDG102, a H3K36-Methyltransferase-Encoding Gene, Plays Pleiotropic Roles in Growth and Development of Maize (<i>Zea mays</i> L.) International Journal of Molecular Sciences maize histone methylation flowering time H3K36 SDG102 |
| title | SDG102, a H3K36-Methyltransferase-Encoding Gene, Plays Pleiotropic Roles in Growth and Development of Maize (<i>Zea mays</i> L.) |
| title_full | SDG102, a H3K36-Methyltransferase-Encoding Gene, Plays Pleiotropic Roles in Growth and Development of Maize (<i>Zea mays</i> L.) |
| title_fullStr | SDG102, a H3K36-Methyltransferase-Encoding Gene, Plays Pleiotropic Roles in Growth and Development of Maize (<i>Zea mays</i> L.) |
| title_full_unstemmed | SDG102, a H3K36-Methyltransferase-Encoding Gene, Plays Pleiotropic Roles in Growth and Development of Maize (<i>Zea mays</i> L.) |
| title_short | SDG102, a H3K36-Methyltransferase-Encoding Gene, Plays Pleiotropic Roles in Growth and Development of Maize (<i>Zea mays</i> L.) |
| title_sort | sdg102 a h3k36 methyltransferase encoding gene plays pleiotropic roles in growth and development of maize i zea mays i l |
| topic | maize histone methylation flowering time H3K36 SDG102 |
| url | https://www.mdpi.com/1422-0067/23/13/7458 |
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