Cloning and expression analysis of DnMSI1 gene in orchid species Dendrobium nobile Lindl
WD40 repeat proteins, the homologs of yeast MSI1, are conserved in plants, participating in protein complexes and playing fundamental functions in plant development. Although several MSI1-like proteins have been cloned and characterized in plants, the roles of MSI1-like proteins in the biennial orna...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2022-12-01
|
| Series: | Plant Signaling & Behavior |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592324.2021.2021649 |
| _version_ | 1797351984464920576 |
|---|---|
| author | Baolu Cui Min Huang Chongdai Guo Ruihong Li Yuqi Wang |
| author_facet | Baolu Cui Min Huang Chongdai Guo Ruihong Li Yuqi Wang |
| author_sort | Baolu Cui |
| collection | DOAJ |
| description | WD40 repeat proteins, the homologs of yeast MSI1, are conserved in plants, participating in protein complexes and playing fundamental functions in plant development. Although several MSI1-like proteins have been cloned and characterized in plants, the roles of MSI1-like proteins in the biennial ornamental plant, Dendrobium nobile Lindl, are still unclear. Here, we report the cloning of the DnMSI1 gene from Dendrobium nobile Lindl with RACE technology. We found that DnMSI1 expression was induced by GA3 and TDZ but inhibited by ABA, PP333, and drought and salt stress. Furthermore, DnMSI1 over-expression in Arabidopsis resulted in decreased tolerance to NaCl stress. These results suggest that DnMSI1 plays negative regulation roles in regulating salinity-stress resistance in Dendrobium nobile Lindl. |
| first_indexed | 2024-03-08T13:08:40Z |
| format | Article |
| id | doaj.art-ba0521e0fd5043679db90021612e8934 |
| institution | Directory Open Access Journal |
| issn | 1559-2316 1559-2324 |
| language | English |
| last_indexed | 2024-03-08T13:08:40Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Signaling & Behavior |
| spelling | doaj.art-ba0521e0fd5043679db90021612e89342024-01-18T15:58:21ZengTaylor & Francis GroupPlant Signaling & Behavior1559-23161559-23242022-12-0117110.1080/15592324.2021.20216492021649Cloning and expression analysis of DnMSI1 gene in orchid species Dendrobium nobile LindlBaolu Cui0Min Huang1Chongdai Guo2Ruihong Li3Yuqi Wang4Qiannan Normal UniversityGuangzhou UniversityGuangzhou UniversityQiannan Normal UniversityGuangzhou UniversityWD40 repeat proteins, the homologs of yeast MSI1, are conserved in plants, participating in protein complexes and playing fundamental functions in plant development. Although several MSI1-like proteins have been cloned and characterized in plants, the roles of MSI1-like proteins in the biennial ornamental plant, Dendrobium nobile Lindl, are still unclear. Here, we report the cloning of the DnMSI1 gene from Dendrobium nobile Lindl with RACE technology. We found that DnMSI1 expression was induced by GA3 and TDZ but inhibited by ABA, PP333, and drought and salt stress. Furthermore, DnMSI1 over-expression in Arabidopsis resulted in decreased tolerance to NaCl stress. These results suggest that DnMSI1 plays negative regulation roles in regulating salinity-stress resistance in Dendrobium nobile Lindl.http://dx.doi.org/10.1080/15592324.2021.2021649dnmsi1cloneexpressionstressdendrobium nobile lindl |
| spellingShingle | Baolu Cui Min Huang Chongdai Guo Ruihong Li Yuqi Wang Cloning and expression analysis of DnMSI1 gene in orchid species Dendrobium nobile Lindl Plant Signaling & Behavior dnmsi1 clone expression stress dendrobium nobile lindl |
| title | Cloning and expression analysis of DnMSI1 gene in orchid species Dendrobium nobile Lindl |
| title_full | Cloning and expression analysis of DnMSI1 gene in orchid species Dendrobium nobile Lindl |
| title_fullStr | Cloning and expression analysis of DnMSI1 gene in orchid species Dendrobium nobile Lindl |
| title_full_unstemmed | Cloning and expression analysis of DnMSI1 gene in orchid species Dendrobium nobile Lindl |
| title_short | Cloning and expression analysis of DnMSI1 gene in orchid species Dendrobium nobile Lindl |
| title_sort | cloning and expression analysis of dnmsi1 gene in orchid species dendrobium nobile lindl |
| topic | dnmsi1 clone expression stress dendrobium nobile lindl |
| url | http://dx.doi.org/10.1080/15592324.2021.2021649 |
| work_keys_str_mv | AT baolucui cloningandexpressionanalysisofdnmsi1geneinorchidspeciesdendrobiumnobilelindl AT minhuang cloningandexpressionanalysisofdnmsi1geneinorchidspeciesdendrobiumnobilelindl AT chongdaiguo cloningandexpressionanalysisofdnmsi1geneinorchidspeciesdendrobiumnobilelindl AT ruihongli cloningandexpressionanalysisofdnmsi1geneinorchidspeciesdendrobiumnobilelindl AT yuqiwang cloningandexpressionanalysisofdnmsi1geneinorchidspeciesdendrobiumnobilelindl |