Multidrug-Resistant <i>Acinetobacter baumannii</i> Genetic Characterization and Spread in Lithuania in 2014, 2016, and 2018
Bacterial resistance to antimicrobial agents plays an important role in the treatment of bacterial infections in healthcare institutions. The spread of multidrug-resistant bacteria can occur during inter- and intra-hospital transmissions among patients and hospital personnel. For this reason, more s...
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MDPI AG
2021-02-01
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| Online Access: | https://www.mdpi.com/2075-1729/11/2/151 |
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| author | Tatjana Kirtikliene Aistė Mierauskaitė Ilona Razmienė Nomeda Kuisiene |
| author_facet | Tatjana Kirtikliene Aistė Mierauskaitė Ilona Razmienė Nomeda Kuisiene |
| author_sort | Tatjana Kirtikliene |
| collection | DOAJ |
| description | Bacterial resistance to antimicrobial agents plays an important role in the treatment of bacterial infections in healthcare institutions. The spread of multidrug-resistant bacteria can occur during inter- and intra-hospital transmissions among patients and hospital personnel. For this reason, more studies must be conducted to understand how resistance occurs in bacteria and how it moves between hospitals by comparing data from different years and looking out for any patterns that might emerge. Multidrug-resistant (MDR) <i>Acinetobacter spp.</i> was studied at 14 healthcare institutions in Lithuania during 2014, 2016, and 2018 using samples from human bloodstream infections. In total, 194 isolates were collected and identified using MALDI-TOF and VITEK2 analyzers as <i>Acinetobacter baumannii</i> group bacteria. After that, the isolates were analyzed for the presence of different resistance genes (20 genes were analyzed) and characterized by using the Rep-PCR and MLVA (multiple-locus variable-number tandem repeat analysis) genotyping methods. The results of the study showed the relatedness of the different <i>Acinetobacter spp</i>. isolates and a possible circulation of resistance genes or profiles during the different years of the study. This study provides essential information, such as variability and diversity of resistance genes, genetic profiling, and clustering of isolates, to better understand the antimicrobial resistance patterns of <i>Acinetobacter spp</i>. These results can be used to strengthen the control of multidrug-resistant infections in healthcare institutions and to prevent potential outbreaks of this pathogen in the future. |
| first_indexed | 2024-03-09T00:49:52Z |
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| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T00:49:52Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-ba05658a9dcd4b1888864d54ed9b53bb2023-12-11T17:17:19ZengMDPI AGLife2075-17292021-02-0111215110.3390/life11020151Multidrug-Resistant <i>Acinetobacter baumannii</i> Genetic Characterization and Spread in Lithuania in 2014, 2016, and 2018Tatjana Kirtikliene0Aistė Mierauskaitė1Ilona Razmienė2Nomeda Kuisiene3Department of Microbiology and Biotechnology, Institute of Biosciences, Life Sciences Center, Vilnius University, LT-10257 Vilnius, LithuaniaNational Public Health Surveillance Laboratory, Clinical Testing Department, LT-10257 Vilnius, LithuaniaNational Public Health Surveillance Laboratory, Clinical Testing Department, LT-10257 Vilnius, LithuaniaDepartment of Microbiology and Biotechnology, Institute of Biosciences, Life Sciences Center, Vilnius University, LT-10257 Vilnius, LithuaniaBacterial resistance to antimicrobial agents plays an important role in the treatment of bacterial infections in healthcare institutions. The spread of multidrug-resistant bacteria can occur during inter- and intra-hospital transmissions among patients and hospital personnel. For this reason, more studies must be conducted to understand how resistance occurs in bacteria and how it moves between hospitals by comparing data from different years and looking out for any patterns that might emerge. Multidrug-resistant (MDR) <i>Acinetobacter spp.</i> was studied at 14 healthcare institutions in Lithuania during 2014, 2016, and 2018 using samples from human bloodstream infections. In total, 194 isolates were collected and identified using MALDI-TOF and VITEK2 analyzers as <i>Acinetobacter baumannii</i> group bacteria. After that, the isolates were analyzed for the presence of different resistance genes (20 genes were analyzed) and characterized by using the Rep-PCR and MLVA (multiple-locus variable-number tandem repeat analysis) genotyping methods. The results of the study showed the relatedness of the different <i>Acinetobacter spp</i>. isolates and a possible circulation of resistance genes or profiles during the different years of the study. This study provides essential information, such as variability and diversity of resistance genes, genetic profiling, and clustering of isolates, to better understand the antimicrobial resistance patterns of <i>Acinetobacter spp</i>. These results can be used to strengthen the control of multidrug-resistant infections in healthcare institutions and to prevent potential outbreaks of this pathogen in the future.https://www.mdpi.com/2075-1729/11/2/151multidrug resistancenosocomial infections<i>Acinetobacter baumannii</i>MLVA-8resistance genes |
| spellingShingle | Tatjana Kirtikliene Aistė Mierauskaitė Ilona Razmienė Nomeda Kuisiene Multidrug-Resistant <i>Acinetobacter baumannii</i> Genetic Characterization and Spread in Lithuania in 2014, 2016, and 2018 Life multidrug resistance nosocomial infections <i>Acinetobacter baumannii</i> MLVA-8 resistance genes |
| title | Multidrug-Resistant <i>Acinetobacter baumannii</i> Genetic Characterization and Spread in Lithuania in 2014, 2016, and 2018 |
| title_full | Multidrug-Resistant <i>Acinetobacter baumannii</i> Genetic Characterization and Spread in Lithuania in 2014, 2016, and 2018 |
| title_fullStr | Multidrug-Resistant <i>Acinetobacter baumannii</i> Genetic Characterization and Spread in Lithuania in 2014, 2016, and 2018 |
| title_full_unstemmed | Multidrug-Resistant <i>Acinetobacter baumannii</i> Genetic Characterization and Spread in Lithuania in 2014, 2016, and 2018 |
| title_short | Multidrug-Resistant <i>Acinetobacter baumannii</i> Genetic Characterization and Spread in Lithuania in 2014, 2016, and 2018 |
| title_sort | multidrug resistant i acinetobacter baumannii i genetic characterization and spread in lithuania in 2014 2016 and 2018 |
| topic | multidrug resistance nosocomial infections <i>Acinetobacter baumannii</i> MLVA-8 resistance genes |
| url | https://www.mdpi.com/2075-1729/11/2/151 |
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