Propagation and shelf-life of weed pathogenic fungi in alternative media and their effectiveness in billygoat (Ageratum conyzoides L)
Control of billygoat (Ageratum conyzoides L) currently uses herbicides, on the other hand, control using pathogenic fungi is environmentally friendly, but for mass propagation and storage, alternative media are needed. The aim of the study was to determine the best type of alternative media for fung...
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| Format: | Article |
| Language: | English |
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indonesian research institute for biotechnology and bioindustry
2023-10-01
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| Series: | Menara Perkebunan |
| Subjects: | |
| Online Access: | http://mp.iribb.org/mpjurnal/article/view/536 |
| _version_ | 1797635367304691712 |
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| author | Loekas Soesanto Murti Wisnu Ragil Sastyawan Abdul Manan Endang Mugiastuti |
| author_facet | Loekas Soesanto Murti Wisnu Ragil Sastyawan Abdul Manan Endang Mugiastuti |
| author_sort | Loekas Soesanto |
| collection | DOAJ |
| description | Control of billygoat (Ageratum conyzoides L) currently uses herbicides, on the other hand, control using pathogenic fungi is environmentally friendly, but for mass propagation and storage, alternative media are needed. The aim of the study was to determine the best type of alternative media for fungal growth and shelf life, as well as its effectiveness against billygoat. The treatment tested involves a combination of two factors: the type of pathogenic fungi (Curvularia lunata or Fusarium oxysporum) and the type of medium (rice washing water or tofu liquid waste). A completely randomized design was used for the in vitro test, while in planta test used a randomized block design with each experimental unit repeated five times. The observed variables were conidia density, number of colonies, incubation period, disease symptoms, disease intensity, and area under the disease progress curve (AUDPC), as well as weed height, number of leaves, fresh and dry shoot, and root weights. The results showed that the conidia density of F. oxysporum was 57% better in rice washing water than in tofu wastewater. The best shelf life for the fungus was four weeks. The use of rice washing water for F. oxysporum and C. lunata effectively delayed the incubation period by 77 and 71% respectively, suppressed disease intensity by 90 and 88%, and AUDPC by 94 and 93% compared to the control. The F. oxysporum grown on rice washing water media was able to reduce the number of leaves, fresh and dry weight of billygoat by 25, 30, and 20% compared to the control, respectively. |
| first_indexed | 2024-03-11T12:20:06Z |
| format | Article |
| id | doaj.art-ba0a68e5e9794793a3308763551c35eb |
| institution | Directory Open Access Journal |
| issn | 0125-9318 1858-3768 |
| language | English |
| last_indexed | 2024-03-11T12:20:06Z |
| publishDate | 2023-10-01 |
| publisher | indonesian research institute for biotechnology and bioindustry |
| record_format | Article |
| series | Menara Perkebunan |
| spelling | doaj.art-ba0a68e5e9794793a3308763551c35eb2023-11-07T02:35:16Zengindonesian research institute for biotechnology and bioindustryMenara Perkebunan0125-93181858-37682023-10-01912149160https://doi.org/10.22302/e-journalmenaraperkebunan.v91i2.536Propagation and shelf-life of weed pathogenic fungi in alternative media and their effectiveness in billygoat (Ageratum conyzoides L)Loekas Soesanto0https://orcid.org/0000-0002-6501-4177Murti Wisnu Ragil Sastyawan1Abdul Manan2Endang Mugiastuti3Department of Agrotechnology, Faculty of Agriculture, Jenderal Soedirman UniversityDepartment of Industrial Technique, Faculty of Technique, Jenderal Soedirman UniversityDepartment of Agrotechnology, Faculty of Agriculture, Jenderal Soedirman UniversityDepartment of Agrotechnology, Faculty of Agriculture, Jenderal Soedirman UniversityControl of billygoat (Ageratum conyzoides L) currently uses herbicides, on the other hand, control using pathogenic fungi is environmentally friendly, but for mass propagation and storage, alternative media are needed. The aim of the study was to determine the best type of alternative media for fungal growth and shelf life, as well as its effectiveness against billygoat. The treatment tested involves a combination of two factors: the type of pathogenic fungi (Curvularia lunata or Fusarium oxysporum) and the type of medium (rice washing water or tofu liquid waste). A completely randomized design was used for the in vitro test, while in planta test used a randomized block design with each experimental unit repeated five times. The observed variables were conidia density, number of colonies, incubation period, disease symptoms, disease intensity, and area under the disease progress curve (AUDPC), as well as weed height, number of leaves, fresh and dry shoot, and root weights. The results showed that the conidia density of F. oxysporum was 57% better in rice washing water than in tofu wastewater. The best shelf life for the fungus was four weeks. The use of rice washing water for F. oxysporum and C. lunata effectively delayed the incubation period by 77 and 71% respectively, suppressed disease intensity by 90 and 88%, and AUDPC by 94 and 93% compared to the control. The F. oxysporum grown on rice washing water media was able to reduce the number of leaves, fresh and dry weight of billygoat by 25, 30, and 20% compared to the control, respectively.http://mp.iribb.org/mpjurnal/article/view/536biological controlbroad leaves weedfungal propagationrice washing waterpreservation |
| spellingShingle | Loekas Soesanto Murti Wisnu Ragil Sastyawan Abdul Manan Endang Mugiastuti Propagation and shelf-life of weed pathogenic fungi in alternative media and their effectiveness in billygoat (Ageratum conyzoides L) Menara Perkebunan biological control broad leaves weed fungal propagation rice washing water preservation |
| title | Propagation and shelf-life of weed pathogenic fungi in alternative media and their effectiveness in billygoat (Ageratum conyzoides L) |
| title_full | Propagation and shelf-life of weed pathogenic fungi in alternative media and their effectiveness in billygoat (Ageratum conyzoides L) |
| title_fullStr | Propagation and shelf-life of weed pathogenic fungi in alternative media and their effectiveness in billygoat (Ageratum conyzoides L) |
| title_full_unstemmed | Propagation and shelf-life of weed pathogenic fungi in alternative media and their effectiveness in billygoat (Ageratum conyzoides L) |
| title_short | Propagation and shelf-life of weed pathogenic fungi in alternative media and their effectiveness in billygoat (Ageratum conyzoides L) |
| title_sort | propagation and shelf life of weed pathogenic fungi in alternative media and their effectiveness in billygoat ageratum conyzoides l |
| topic | biological control broad leaves weed fungal propagation rice washing water preservation |
| url | http://mp.iribb.org/mpjurnal/article/view/536 |
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