An oviposition stimulant binding protein in a butterfly <subtitle>Immunohistochemical localization and electrophysiological responses to plant compounds</subtitle>
Oviposition is evoked by plant compounds, which are recognized by chemoreceptive organs of insects. The swallowtail butterfly, Atrophaneura alcinous, oviposits its eggs on the host plant, Aristolochia debilis, in the presence of only two stimulating compounds: an alkaloid, aristolochic acid, and a m...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2009-07-01
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| Series: | Communicative & Integrative Biology |
| Online Access: | https://www.tandfonline.com/doi/10.4161/cib.2.4.8613 |
| _version_ | 1797683957846769664 |
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| author | Kazuko Tsuchihara Osamu Hisatomi Fumio Tokunaga Kiyoshi Asaoka |
| author_facet | Kazuko Tsuchihara Osamu Hisatomi Fumio Tokunaga Kiyoshi Asaoka |
| author_sort | Kazuko Tsuchihara |
| collection | DOAJ |
| description | Oviposition is evoked by plant compounds, which are recognized by chemoreceptive organs of insects. The swallowtail butterfly, Atrophaneura alcinous, oviposits its eggs on the host plant, Aristolochia debilis, in the presence of only two stimulating compounds: an alkaloid, aristolochic acid, and a monosaccharide, sequoyitol. In our previous study, a unique protein of 23 kDa [Oviposition stimulant(s) binding protein (OSBP)] was found in the forelegs of female, but not male, A. alcinous. The electrophysiological response of A. alcinous to an extract of A. debilis was depressed by the presence of OSBP antiserum, suggesting that OSBP presumably binds to oviposition stimulant(s). We show here, using a highly sensitive fluorescence micro-binding assay that native OSBP binds to a main oviposition stimulant, aristolochic acid, from its host plant, A. debilis. Three-dimensional molecular modeling studies also gave a reasonable structure for the OSBP / aristolochic acid complex. This is the first report of a native chemoreceptive protein binding to an oviposition stimulant ligand in insects. |
| first_indexed | 2024-03-12T00:22:24Z |
| format | Article |
| id | doaj.art-ba0d1d8237714fd08891c8b10b8568df |
| institution | Directory Open Access Journal |
| issn | 1942-0889 |
| language | English |
| last_indexed | 2024-03-12T00:22:24Z |
| publishDate | 2009-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Communicative & Integrative Biology |
| spelling | doaj.art-ba0d1d8237714fd08891c8b10b8568df2023-09-15T13:02:39ZengTaylor & Francis GroupCommunicative & Integrative Biology1942-08892009-07-012435635810.4161/cib.2.4.8613An oviposition stimulant binding protein in a butterfly <subtitle>Immunohistochemical localization and electrophysiological responses to plant compounds</subtitle>Kazuko Tsuchihara0Osamu Hisatomi1Fumio Tokunaga2Kiyoshi Asaoka3Iwaki Meisei University; Iwaki, JapanDepartment of Biological Sciences; Graduate School of Science; Osaka University; Osaka, JapanDepartment of Biological Sciences; Graduate School of Science; Osaka University; Osaka, JapanNational Institute of Agrobiological Sciences; Tsukuba, Ibaraki, JapanOviposition is evoked by plant compounds, which are recognized by chemoreceptive organs of insects. The swallowtail butterfly, Atrophaneura alcinous, oviposits its eggs on the host plant, Aristolochia debilis, in the presence of only two stimulating compounds: an alkaloid, aristolochic acid, and a monosaccharide, sequoyitol. In our previous study, a unique protein of 23 kDa [Oviposition stimulant(s) binding protein (OSBP)] was found in the forelegs of female, but not male, A. alcinous. The electrophysiological response of A. alcinous to an extract of A. debilis was depressed by the presence of OSBP antiserum, suggesting that OSBP presumably binds to oviposition stimulant(s). We show here, using a highly sensitive fluorescence micro-binding assay that native OSBP binds to a main oviposition stimulant, aristolochic acid, from its host plant, A. debilis. Three-dimensional molecular modeling studies also gave a reasonable structure for the OSBP / aristolochic acid complex. This is the first report of a native chemoreceptive protein binding to an oviposition stimulant ligand in insects.https://www.tandfonline.com/doi/10.4161/cib.2.4.8613 |
| spellingShingle | Kazuko Tsuchihara Osamu Hisatomi Fumio Tokunaga Kiyoshi Asaoka An oviposition stimulant binding protein in a butterfly <subtitle>Immunohistochemical localization and electrophysiological responses to plant compounds</subtitle> Communicative & Integrative Biology |
| title | An oviposition stimulant binding protein in a butterfly <subtitle>Immunohistochemical localization and electrophysiological responses to plant compounds</subtitle> |
| title_full | An oviposition stimulant binding protein in a butterfly <subtitle>Immunohistochemical localization and electrophysiological responses to plant compounds</subtitle> |
| title_fullStr | An oviposition stimulant binding protein in a butterfly <subtitle>Immunohistochemical localization and electrophysiological responses to plant compounds</subtitle> |
| title_full_unstemmed | An oviposition stimulant binding protein in a butterfly <subtitle>Immunohistochemical localization and electrophysiological responses to plant compounds</subtitle> |
| title_short | An oviposition stimulant binding protein in a butterfly <subtitle>Immunohistochemical localization and electrophysiological responses to plant compounds</subtitle> |
| title_sort | oviposition stimulant binding protein in a butterfly subtitle immunohistochemical localization and electrophysiological responses to plant compounds subtitle |
| url | https://www.tandfonline.com/doi/10.4161/cib.2.4.8613 |
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