Reduced glutathione and glutathione disulfide in the blood of glucose-6-phosphate dehydrogenase-deficient newborns
Abstract Background Glucose-6-phosphate dehydrogenase (G6PD) deficiency is commonly detected during mass screening for neonatal disease. We developed a method to measure reduced glutathione (GSH) and glutathione disulfide (GSSG) using tandem mass spectrometry (MS/MS) for detecting G6PD deficiency. M...
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| Language: | English |
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BMC
2017-07-01
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| Series: | BMC Pediatrics |
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| Online Access: | http://link.springer.com/article/10.1186/s12887-017-0920-y |
| _version_ | 1811280868408295424 |
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| author | Zhen-hua Gong Guo-li Tian Qi-wei Huang Yan-min Wang Hong-ping Xu |
| author_facet | Zhen-hua Gong Guo-li Tian Qi-wei Huang Yan-min Wang Hong-ping Xu |
| author_sort | Zhen-hua Gong |
| collection | DOAJ |
| description | Abstract Background Glucose-6-phosphate dehydrogenase (G6PD) deficiency is commonly detected during mass screening for neonatal disease. We developed a method to measure reduced glutathione (GSH) and glutathione disulfide (GSSG) using tandem mass spectrometry (MS/MS) for detecting G6PD deficiency. Methods The concentration of GSH and the GSH/GSSG ratio in newborn dry-blood-spot (DBS) screening and in blood plus sodium citrate for test confirmation were examined by MS/MS using labeled glycine as an internal standard. Results G6PD-deficient newborns had a lower GSH content (242.9 ± 15.9 μmol/L)and GSH/GSSG ratio (14.9 ± 7.2) than neonatal controls (370.0 ± 53.2 μmol/L and 46.7 ± 19.6, respectively). Although the results showed a significance of P < 0.001 for DBS samples plus sodium citrate that were examined the first day after preparation, there were no significant differences in the mean GSH concentration and GSH/GSSG ratio between the G6PD deficiency-positive and negative groups when examined three days after sample preparation. Conclusion The concentration of GSH and the ratio of GSH/GSSG in blood measured using MS/MS on the first day of sample preparation are consistent with G6PD activity and are helpful for diagnosing G6PD deficiency. |
| first_indexed | 2024-04-13T01:23:33Z |
| format | Article |
| id | doaj.art-ba0daf0aaa58432e9f330b7d4ba9bd24 |
| institution | Directory Open Access Journal |
| issn | 1471-2431 |
| language | English |
| last_indexed | 2024-04-13T01:23:33Z |
| publishDate | 2017-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pediatrics |
| spelling | doaj.art-ba0daf0aaa58432e9f330b7d4ba9bd242022-12-22T03:08:42ZengBMCBMC Pediatrics1471-24312017-07-011711610.1186/s12887-017-0920-yReduced glutathione and glutathione disulfide in the blood of glucose-6-phosphate dehydrogenase-deficient newbornsZhen-hua Gong0Guo-li Tian1Qi-wei Huang2Yan-min Wang3Hong-ping Xu4Department of general surgery, Shanghai Children’s Hospital, Shanghai Jiao Tong UniversityNeonatal screening center, Shanghai Children’s Hospital, Shanghai Jiao Tong UniversityDepartment of neonatology, Shanghai Children’s Hospital, Shanghai Jiao Tong UniversityNeonatal screening center, Shanghai Children’s Hospital, Shanghai Jiao Tong UniversityNeonatal screening center, Shanghai Children’s Hospital, Shanghai Jiao Tong UniversityAbstract Background Glucose-6-phosphate dehydrogenase (G6PD) deficiency is commonly detected during mass screening for neonatal disease. We developed a method to measure reduced glutathione (GSH) and glutathione disulfide (GSSG) using tandem mass spectrometry (MS/MS) for detecting G6PD deficiency. Methods The concentration of GSH and the GSH/GSSG ratio in newborn dry-blood-spot (DBS) screening and in blood plus sodium citrate for test confirmation were examined by MS/MS using labeled glycine as an internal standard. Results G6PD-deficient newborns had a lower GSH content (242.9 ± 15.9 μmol/L)and GSH/GSSG ratio (14.9 ± 7.2) than neonatal controls (370.0 ± 53.2 μmol/L and 46.7 ± 19.6, respectively). Although the results showed a significance of P < 0.001 for DBS samples plus sodium citrate that were examined the first day after preparation, there were no significant differences in the mean GSH concentration and GSH/GSSG ratio between the G6PD deficiency-positive and negative groups when examined three days after sample preparation. Conclusion The concentration of GSH and the ratio of GSH/GSSG in blood measured using MS/MS on the first day of sample preparation are consistent with G6PD activity and are helpful for diagnosing G6PD deficiency.http://link.springer.com/article/10.1186/s12887-017-0920-yGlucose-6-phosphate dehydrogenase deficiencyBloodGlutathioneTandem mass spectrometry |
| spellingShingle | Zhen-hua Gong Guo-li Tian Qi-wei Huang Yan-min Wang Hong-ping Xu Reduced glutathione and glutathione disulfide in the blood of glucose-6-phosphate dehydrogenase-deficient newborns BMC Pediatrics Glucose-6-phosphate dehydrogenase deficiency Blood Glutathione Tandem mass spectrometry |
| title | Reduced glutathione and glutathione disulfide in the blood of glucose-6-phosphate dehydrogenase-deficient newborns |
| title_full | Reduced glutathione and glutathione disulfide in the blood of glucose-6-phosphate dehydrogenase-deficient newborns |
| title_fullStr | Reduced glutathione and glutathione disulfide in the blood of glucose-6-phosphate dehydrogenase-deficient newborns |
| title_full_unstemmed | Reduced glutathione and glutathione disulfide in the blood of glucose-6-phosphate dehydrogenase-deficient newborns |
| title_short | Reduced glutathione and glutathione disulfide in the blood of glucose-6-phosphate dehydrogenase-deficient newborns |
| title_sort | reduced glutathione and glutathione disulfide in the blood of glucose 6 phosphate dehydrogenase deficient newborns |
| topic | Glucose-6-phosphate dehydrogenase deficiency Blood Glutathione Tandem mass spectrometry |
| url | http://link.springer.com/article/10.1186/s12887-017-0920-y |
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