Vector competence of Aedes aegypti and screening for differentially expressed microRNAs exposed to Zika virus
Abstract Background Zika virus (ZIKV) is transmitted to humans primarily by Aedes aegypti. Previous studies on Ae. aegypti from Jiegao (JG) and Mengding (MD) in Yunnan province, China have shown that these mosquitoes are able to transmit ZIKV to their offspring through vertical transmission, indicat...
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| Format: | Article |
| Language: | English |
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BMC
2021-09-01
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| Series: | Parasites & Vectors |
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| Online Access: | https://doi.org/10.1186/s13071-021-05007-7 |
| _version_ | 1818717238150037504 |
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| author | Chunling Zhu Yuting Jiang Qianghui Zhang Jian Gao Chaojie Li Chunxiao Li Yande Dong Dan Xing Hengduan Zhang Teng Zhao Xiaoxia Guo Tongyan Zhao |
| author_facet | Chunling Zhu Yuting Jiang Qianghui Zhang Jian Gao Chaojie Li Chunxiao Li Yande Dong Dan Xing Hengduan Zhang Teng Zhao Xiaoxia Guo Tongyan Zhao |
| author_sort | Chunling Zhu |
| collection | DOAJ |
| description | Abstract Background Zika virus (ZIKV) is transmitted to humans primarily by Aedes aegypti. Previous studies on Ae. aegypti from Jiegao (JG) and Mengding (MD) in Yunnan province, China have shown that these mosquitoes are able to transmit ZIKV to their offspring through vertical transmission, indicating that these two Ae. aegypti strains pose a potential risk for ZIKV transmission. However, the vector competence of these two Ae. aegypti strains to ZIKV has not been evaluated and the molecular mechanisms influencing vector competence are still unclear. Methods Aedes aegypti mosquitoes from JG and MD were orally infected with ZIKV, and the infection rate (IR), dissemination rate (DR), transmission rate (TR) and transmission efficiency (TE) of these two mosquito strains were explored to evaluate their vector competence to ZIKV. On 2, 4 and 6 days post-infection (dpi), the small RNA profiles between ZIKV-infected and non-infected Ae. aegypti midgut and salivary gland tissues were compared to gain insights into the molecular interactions between ZIKV and Ae. aegypti. Results There were no significant differences in the IR, DR, TR and TE between the two Ae. aegypti strains (P > 0.05). However, ZIKV RNA appeared 2 days earlier in saliva of the JG strain, which indicated a higher competence of the JG strain to transmit ZIKV. Significant differences in the microRNA (miRNA) expression profiles between ZIKV-infected and non-infected Ae. aegypti were found in the 2-dpi libraries of both the midgut and salivary gland tissues from the two strains. In addition, 27 and 74 miRNAs (|log2 fold change| > 2) were selected from the miRNA expression profiles of ZIKV-infected and non-infected midgut and salivary gland tissues from the JG and MD strains, respectively. Conclusions Our results provide novel insights into the ZIKV–mosquito interactions and build a foundation for future research on how miRNAs regulate the vector competence of mosquitoes to this arbovirus. Graphical abstract |
| first_indexed | 2024-12-17T19:31:58Z |
| format | Article |
| id | doaj.art-ba0db298fb5a4d8eb364367d45b8f6ef |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-17T19:31:58Z |
| publishDate | 2021-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-ba0db298fb5a4d8eb364367d45b8f6ef2022-12-21T21:35:14ZengBMCParasites & Vectors1756-33052021-09-0114111510.1186/s13071-021-05007-7Vector competence of Aedes aegypti and screening for differentially expressed microRNAs exposed to Zika virusChunling Zhu0Yuting Jiang1Qianghui Zhang2Jian Gao3Chaojie Li4Chunxiao Li5Yande Dong6Dan Xing7Hengduan Zhang8Teng Zhao9Xiaoxia Guo10Tongyan Zhao11Department of Vector Biology and Control, State Key Laboratory of Pathogen and Biosecurity, Beijing Key Laboratory of Vector Borne and Natural Focus Infectious Diseases, Beijing Institute of Microbiology and EpidemiologyDepartment of Vector Biology and Control, State Key Laboratory of Pathogen and Biosecurity, Beijing Key Laboratory of Vector Borne and Natural Focus Infectious Diseases, Beijing Institute of Microbiology and EpidemiologyDepartment of Vector Biology and Control, State Key Laboratory of Pathogen and Biosecurity, Beijing Key Laboratory of Vector Borne and Natural Focus Infectious Diseases, Beijing Institute of Microbiology and EpidemiologyDepartment of Vector Biology and Control, State Key Laboratory of Pathogen and Biosecurity, Beijing Key Laboratory of Vector Borne and Natural Focus Infectious Diseases, Beijing Institute of Microbiology and EpidemiologyDepartment of Vector Biology and Control, State Key Laboratory of Pathogen and Biosecurity, Beijing Key Laboratory of Vector Borne and Natural Focus Infectious Diseases, Beijing Institute of Microbiology and EpidemiologyDepartment of Vector Biology and Control, State Key Laboratory of Pathogen and Biosecurity, Beijing Key Laboratory of Vector Borne and Natural Focus Infectious Diseases, Beijing Institute of Microbiology and EpidemiologyDepartment of Vector Biology and Control, State Key Laboratory of Pathogen and Biosecurity, Beijing Key Laboratory of Vector Borne and Natural Focus Infectious Diseases, Beijing Institute of Microbiology and EpidemiologyDepartment of Vector Biology and Control, State Key Laboratory of Pathogen and Biosecurity, Beijing Key Laboratory of Vector Borne and Natural Focus Infectious Diseases, Beijing Institute of Microbiology and EpidemiologyDepartment of Vector Biology and Control, State Key Laboratory of Pathogen and Biosecurity, Beijing Key Laboratory of Vector Borne and Natural Focus Infectious Diseases, Beijing Institute of Microbiology and EpidemiologyDepartment of Vector Biology and Control, State Key Laboratory of Pathogen and Biosecurity, Beijing Key Laboratory of Vector Borne and Natural Focus Infectious Diseases, Beijing Institute of Microbiology and EpidemiologyDepartment of Vector Biology and Control, State Key Laboratory of Pathogen and Biosecurity, Beijing Key Laboratory of Vector Borne and Natural Focus Infectious Diseases, Beijing Institute of Microbiology and EpidemiologyDepartment of Vector Biology and Control, State Key Laboratory of Pathogen and Biosecurity, Beijing Key Laboratory of Vector Borne and Natural Focus Infectious Diseases, Beijing Institute of Microbiology and EpidemiologyAbstract Background Zika virus (ZIKV) is transmitted to humans primarily by Aedes aegypti. Previous studies on Ae. aegypti from Jiegao (JG) and Mengding (MD) in Yunnan province, China have shown that these mosquitoes are able to transmit ZIKV to their offspring through vertical transmission, indicating that these two Ae. aegypti strains pose a potential risk for ZIKV transmission. However, the vector competence of these two Ae. aegypti strains to ZIKV has not been evaluated and the molecular mechanisms influencing vector competence are still unclear. Methods Aedes aegypti mosquitoes from JG and MD were orally infected with ZIKV, and the infection rate (IR), dissemination rate (DR), transmission rate (TR) and transmission efficiency (TE) of these two mosquito strains were explored to evaluate their vector competence to ZIKV. On 2, 4 and 6 days post-infection (dpi), the small RNA profiles between ZIKV-infected and non-infected Ae. aegypti midgut and salivary gland tissues were compared to gain insights into the molecular interactions between ZIKV and Ae. aegypti. Results There were no significant differences in the IR, DR, TR and TE between the two Ae. aegypti strains (P > 0.05). However, ZIKV RNA appeared 2 days earlier in saliva of the JG strain, which indicated a higher competence of the JG strain to transmit ZIKV. Significant differences in the microRNA (miRNA) expression profiles between ZIKV-infected and non-infected Ae. aegypti were found in the 2-dpi libraries of both the midgut and salivary gland tissues from the two strains. In addition, 27 and 74 miRNAs (|log2 fold change| > 2) were selected from the miRNA expression profiles of ZIKV-infected and non-infected midgut and salivary gland tissues from the JG and MD strains, respectively. Conclusions Our results provide novel insights into the ZIKV–mosquito interactions and build a foundation for future research on how miRNAs regulate the vector competence of mosquitoes to this arbovirus. Graphical abstracthttps://doi.org/10.1186/s13071-021-05007-7Zika virusAedes aegyptiVector competencemicroRNA |
| spellingShingle | Chunling Zhu Yuting Jiang Qianghui Zhang Jian Gao Chaojie Li Chunxiao Li Yande Dong Dan Xing Hengduan Zhang Teng Zhao Xiaoxia Guo Tongyan Zhao Vector competence of Aedes aegypti and screening for differentially expressed microRNAs exposed to Zika virus Parasites & Vectors Zika virus Aedes aegypti Vector competence microRNA |
| title | Vector competence of Aedes aegypti and screening for differentially expressed microRNAs exposed to Zika virus |
| title_full | Vector competence of Aedes aegypti and screening for differentially expressed microRNAs exposed to Zika virus |
| title_fullStr | Vector competence of Aedes aegypti and screening for differentially expressed microRNAs exposed to Zika virus |
| title_full_unstemmed | Vector competence of Aedes aegypti and screening for differentially expressed microRNAs exposed to Zika virus |
| title_short | Vector competence of Aedes aegypti and screening for differentially expressed microRNAs exposed to Zika virus |
| title_sort | vector competence of aedes aegypti and screening for differentially expressed micrornas exposed to zika virus |
| topic | Zika virus Aedes aegypti Vector competence microRNA |
| url | https://doi.org/10.1186/s13071-021-05007-7 |
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