Transient gene expression of beta-glucuronidase in citrus thin epicotyl transversal sections using particle bombardment
Studies were carried out to optimize the conditions for transient gene expression through particle bombardment on Carrizo citrange (Citrus sinensis x Poncirus trifoliata) thin epicotyl sections. The best conditions for transient GUS expression were: M-25 tungsten particles, 1550 psi helium pressure,...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2003-01-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100001&tlng=en |
| _version_ | 1818972353702395904 |
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| author | João C. Bespalhok Filho Adilson K. Kobayashi Luiz F. P. Pereira Rafaelo M. Galvão Luiz G. E. Vieira |
| author_facet | João C. Bespalhok Filho Adilson K. Kobayashi Luiz F. P. Pereira Rafaelo M. Galvão Luiz G. E. Vieira |
| author_sort | João C. Bespalhok Filho |
| collection | DOAJ |
| description | Studies were carried out to optimize the conditions for transient gene expression through particle bombardment on Carrizo citrange (Citrus sinensis x Poncirus trifoliata) thin epicotyl sections. The best conditions for transient GUS expression were: M-25 tungsten particles, 1550 psi helium pressure, 9 cm distance between specimen and DNA/particle holder and culture of explants in a high osmolarity medium (0.2 M mannitol + 0.2 M sorbitol) 4 h prior and 20 h after bombardment. Under these conditions, an average of 102 blue spots per bombardment (20 explants/plate) were achieved. This protocol is currently being used for transformation of Carrizo citrange and sweet orange (Citrus sinensis). |
| first_indexed | 2024-12-20T15:06:55Z |
| format | Article |
| id | doaj.art-ba0f1c3d09154167ae4ff1597ae995c3 |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-12-20T15:06:55Z |
| publishDate | 2003-01-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-ba0f1c3d09154167ae4ff1597ae995c32022-12-21T19:36:26ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242003-01-0146116Transient gene expression of beta-glucuronidase in citrus thin epicotyl transversal sections using particle bombardmentJoão C. Bespalhok Filho0Adilson K. Kobayashi1Luiz F. P. Pereira2Rafaelo M. Galvão3Luiz G. E. Vieira4Instituto Agronômico do ParanáInstituto Agronômico do ParanáInstituto Agronômico do ParanáInstituto Agronômico do ParanáInstituto Agronômico do ParanáStudies were carried out to optimize the conditions for transient gene expression through particle bombardment on Carrizo citrange (Citrus sinensis x Poncirus trifoliata) thin epicotyl sections. The best conditions for transient GUS expression were: M-25 tungsten particles, 1550 psi helium pressure, 9 cm distance between specimen and DNA/particle holder and culture of explants in a high osmolarity medium (0.2 M mannitol + 0.2 M sorbitol) 4 h prior and 20 h after bombardment. Under these conditions, an average of 102 blue spots per bombardment (20 explants/plate) were achieved. This protocol is currently being used for transformation of Carrizo citrange and sweet orange (Citrus sinensis).http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100001&tlng=enTransformationuidA expressionbiolisticstransgenic citrus |
| spellingShingle | João C. Bespalhok Filho Adilson K. Kobayashi Luiz F. P. Pereira Rafaelo M. Galvão Luiz G. E. Vieira Transient gene expression of beta-glucuronidase in citrus thin epicotyl transversal sections using particle bombardment Brazilian Archives of Biology and Technology Transformation uidA expression biolistics transgenic citrus |
| title | Transient gene expression of beta-glucuronidase in citrus thin epicotyl transversal sections using particle bombardment |
| title_full | Transient gene expression of beta-glucuronidase in citrus thin epicotyl transversal sections using particle bombardment |
| title_fullStr | Transient gene expression of beta-glucuronidase in citrus thin epicotyl transversal sections using particle bombardment |
| title_full_unstemmed | Transient gene expression of beta-glucuronidase in citrus thin epicotyl transversal sections using particle bombardment |
| title_short | Transient gene expression of beta-glucuronidase in citrus thin epicotyl transversal sections using particle bombardment |
| title_sort | transient gene expression of beta glucuronidase in citrus thin epicotyl transversal sections using particle bombardment |
| topic | Transformation uidA expression biolistics transgenic citrus |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100001&tlng=en |
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