Comprehensive profiling of neutralizing polyclonal sera targeting coxsackievirus B3
Abstract Despite their fundamental role in resolving viral infections, our understanding of how polyclonal neutralizing antibody responses target non-enveloped viruses remains limited. To define these responses, we obtained the full antigenic profile of multiple human and mouse polyclonal sera targe...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-42144-2 |
| _version_ | 1797558403160080384 |
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| author | Beatriz Álvarez-Rodríguez Javier Buceta Ron Geller |
| author_facet | Beatriz Álvarez-Rodríguez Javier Buceta Ron Geller |
| author_sort | Beatriz Álvarez-Rodríguez |
| collection | DOAJ |
| description | Abstract Despite their fundamental role in resolving viral infections, our understanding of how polyclonal neutralizing antibody responses target non-enveloped viruses remains limited. To define these responses, we obtained the full antigenic profile of multiple human and mouse polyclonal sera targeting the capsid of a prototypical picornavirus, coxsackievirus B3. Our results uncover significant variation in the breadth and strength of neutralization sites targeted by individual human polyclonal responses, which contrasted with homogenous responses observed in experimentally infected mice. We further use these comprehensive antigenic profiles to define key structural and evolutionary parameters that are predictive of escape, assess epitope dominance at the population level, and reveal a need for at least two mutations to achieve significant escape from multiple sera. Overall, our data provide a comprehensive analysis of how polyclonal sera target a non-enveloped viral capsid and help define both immune dominance and escape at the population level. |
| first_indexed | 2024-03-10T17:30:58Z |
| format | Article |
| id | doaj.art-ba12145d1f9947409ff27bc355448036 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-10T17:30:58Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-ba12145d1f9947409ff27bc3554480362023-11-20T10:02:28ZengNature PortfolioNature Communications2041-17232023-10-0114111610.1038/s41467-023-42144-2Comprehensive profiling of neutralizing polyclonal sera targeting coxsackievirus B3Beatriz Álvarez-Rodríguez0Javier Buceta1Ron Geller2Institute for Integrative Systems Biology (I2SysBio), Universitat de Valencia-CSICInstitute for Integrative Systems Biology (I2SysBio), Universitat de Valencia-CSICInstitute for Integrative Systems Biology (I2SysBio), Universitat de Valencia-CSICAbstract Despite their fundamental role in resolving viral infections, our understanding of how polyclonal neutralizing antibody responses target non-enveloped viruses remains limited. To define these responses, we obtained the full antigenic profile of multiple human and mouse polyclonal sera targeting the capsid of a prototypical picornavirus, coxsackievirus B3. Our results uncover significant variation in the breadth and strength of neutralization sites targeted by individual human polyclonal responses, which contrasted with homogenous responses observed in experimentally infected mice. We further use these comprehensive antigenic profiles to define key structural and evolutionary parameters that are predictive of escape, assess epitope dominance at the population level, and reveal a need for at least two mutations to achieve significant escape from multiple sera. Overall, our data provide a comprehensive analysis of how polyclonal sera target a non-enveloped viral capsid and help define both immune dominance and escape at the population level.https://doi.org/10.1038/s41467-023-42144-2 |
| spellingShingle | Beatriz Álvarez-Rodríguez Javier Buceta Ron Geller Comprehensive profiling of neutralizing polyclonal sera targeting coxsackievirus B3 Nature Communications |
| title | Comprehensive profiling of neutralizing polyclonal sera targeting coxsackievirus B3 |
| title_full | Comprehensive profiling of neutralizing polyclonal sera targeting coxsackievirus B3 |
| title_fullStr | Comprehensive profiling of neutralizing polyclonal sera targeting coxsackievirus B3 |
| title_full_unstemmed | Comprehensive profiling of neutralizing polyclonal sera targeting coxsackievirus B3 |
| title_short | Comprehensive profiling of neutralizing polyclonal sera targeting coxsackievirus B3 |
| title_sort | comprehensive profiling of neutralizing polyclonal sera targeting coxsackievirus b3 |
| url | https://doi.org/10.1038/s41467-023-42144-2 |
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