Paeoniflorin inhibits colorectal cancer cell stemness through the miR‐3194‐5p/catenin beta‐interacting protein 1 axis
Abstract Paeoniflorin (PF) is a natural plant ingredient with remarkable antitumor effects. Herein, we investigated the biological effects and mechanism of PF in colorectal cancer (CRC) cell stemness. The messenger RNA (mRNA) and protein expressions were assessed using quantitative real‐time polymer...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-10-01
|
| Series: | Kaohsiung Journal of Medical Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/kjm2.12736 |
| _version_ | 1797663362702639104 |
|---|---|
| author | Zhao Su Beier Hu Jing Li Zhichun Zeng Hu Chen Yuhang Guo Yun Mao Wen Cao |
| author_facet | Zhao Su Beier Hu Jing Li Zhichun Zeng Hu Chen Yuhang Guo Yun Mao Wen Cao |
| author_sort | Zhao Su |
| collection | DOAJ |
| description | Abstract Paeoniflorin (PF) is a natural plant ingredient with remarkable antitumor effects. Herein, we investigated the biological effects and mechanism of PF in colorectal cancer (CRC) cell stemness. The messenger RNA (mRNA) and protein expressions were assessed using quantitative real‐time polymerase chain reaction and western blot. The viability, proliferation, and migration and invasion of CRC cells were evaluated using cell counting kit‐8, clone‐formation, and transwell migration and invasion assays, respectively. The sphere‐formation capacity was determined using the sphere‐formation assay. A dual‐luciferase reporter gene assay was employed to analyze the interaction between miR‐3194‐5p and catenin beta‐interacting protein 1 (CTNNBIP1). The viability, migration, invasion, epithelial–mesenchymal transition, and stemness of CRC cells were repressed by PF. MiR‐3194‐5p was upregulated in CRC tissues and cells. MiR‐3194‐5p knockdown suppressed CRC cell stemness, while miR‐3194‐5p overexpression had the opposite effect. In addition, the inhibition of CRC cell stemness caused by PF was eliminated by miR‐3194‐5p overexpression. CTNNBIP1 functioned as the target of miR‐3194‐5p, whose knockdown abrogated the repression of CRC cell stemness and Wnt/β‐catenin signaling activation by PF.PF regulated the miR‐3194‐5p/CTNNBIP1/Wnt/β‐catenin axis to repress CRC cell stemness. |
| first_indexed | 2024-03-11T19:13:25Z |
| format | Article |
| id | doaj.art-ba1599af69e64cdabdcb9e916b0ba19c |
| institution | Directory Open Access Journal |
| issn | 1607-551X 2410-8650 |
| language | English |
| last_indexed | 2024-03-11T19:13:25Z |
| publishDate | 2023-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-ba1599af69e64cdabdcb9e916b0ba19c2023-10-09T09:20:11ZengWileyKaohsiung Journal of Medical Sciences1607-551X2410-86502023-10-0139101011102110.1002/kjm2.12736Paeoniflorin inhibits colorectal cancer cell stemness through the miR‐3194‐5p/catenin beta‐interacting protein 1 axisZhao Su0Beier Hu1Jing Li2Zhichun Zeng3Hu Chen4Yuhang Guo5Yun Mao6Wen Cao7The Second Affiliated Hospital of Hunan University of Chinese Medicine Changsha Hunan ChinaTumor Hematology Department The Second Affiliated Hospital of Hunan University of Chinese Medicine Changsha Hunan ChinaThe Second Affiliated Hospital of Hunan University of Chinese Medicine Changsha Hunan ChinaThe Second Affiliated Hospital of Hunan University of Chinese Medicine Changsha Hunan ChinaThe Second Affiliated Hospital of Hunan University of Chinese Medicine Changsha Hunan ChinaThe Second Affiliated Hospital of Hunan University of Chinese Medicine Changsha Hunan ChinaTumor Hematology Department The Second Affiliated Hospital of Hunan University of Chinese Medicine Changsha Hunan ChinaTumor Hematology Department The Second Affiliated Hospital of Hunan University of Chinese Medicine Changsha Hunan ChinaAbstract Paeoniflorin (PF) is a natural plant ingredient with remarkable antitumor effects. Herein, we investigated the biological effects and mechanism of PF in colorectal cancer (CRC) cell stemness. The messenger RNA (mRNA) and protein expressions were assessed using quantitative real‐time polymerase chain reaction and western blot. The viability, proliferation, and migration and invasion of CRC cells were evaluated using cell counting kit‐8, clone‐formation, and transwell migration and invasion assays, respectively. The sphere‐formation capacity was determined using the sphere‐formation assay. A dual‐luciferase reporter gene assay was employed to analyze the interaction between miR‐3194‐5p and catenin beta‐interacting protein 1 (CTNNBIP1). The viability, migration, invasion, epithelial–mesenchymal transition, and stemness of CRC cells were repressed by PF. MiR‐3194‐5p was upregulated in CRC tissues and cells. MiR‐3194‐5p knockdown suppressed CRC cell stemness, while miR‐3194‐5p overexpression had the opposite effect. In addition, the inhibition of CRC cell stemness caused by PF was eliminated by miR‐3194‐5p overexpression. CTNNBIP1 functioned as the target of miR‐3194‐5p, whose knockdown abrogated the repression of CRC cell stemness and Wnt/β‐catenin signaling activation by PF.PF regulated the miR‐3194‐5p/CTNNBIP1/Wnt/β‐catenin axis to repress CRC cell stemness.https://doi.org/10.1002/kjm2.12736colorectal cancerCTNNBIP1miR‐3194‐5ppaeoniflorinstemness |
| spellingShingle | Zhao Su Beier Hu Jing Li Zhichun Zeng Hu Chen Yuhang Guo Yun Mao Wen Cao Paeoniflorin inhibits colorectal cancer cell stemness through the miR‐3194‐5p/catenin beta‐interacting protein 1 axis Kaohsiung Journal of Medical Sciences colorectal cancer CTNNBIP1 miR‐3194‐5p paeoniflorin stemness |
| title | Paeoniflorin inhibits colorectal cancer cell stemness through the miR‐3194‐5p/catenin beta‐interacting protein 1 axis |
| title_full | Paeoniflorin inhibits colorectal cancer cell stemness through the miR‐3194‐5p/catenin beta‐interacting protein 1 axis |
| title_fullStr | Paeoniflorin inhibits colorectal cancer cell stemness through the miR‐3194‐5p/catenin beta‐interacting protein 1 axis |
| title_full_unstemmed | Paeoniflorin inhibits colorectal cancer cell stemness through the miR‐3194‐5p/catenin beta‐interacting protein 1 axis |
| title_short | Paeoniflorin inhibits colorectal cancer cell stemness through the miR‐3194‐5p/catenin beta‐interacting protein 1 axis |
| title_sort | paeoniflorin inhibits colorectal cancer cell stemness through the mir 3194 5p catenin beta interacting protein 1 axis |
| topic | colorectal cancer CTNNBIP1 miR‐3194‐5p paeoniflorin stemness |
| url | https://doi.org/10.1002/kjm2.12736 |
| work_keys_str_mv | AT zhaosu paeoniflorininhibitscolorectalcancercellstemnessthroughthemir31945pcateninbetainteractingprotein1axis AT beierhu paeoniflorininhibitscolorectalcancercellstemnessthroughthemir31945pcateninbetainteractingprotein1axis AT jingli paeoniflorininhibitscolorectalcancercellstemnessthroughthemir31945pcateninbetainteractingprotein1axis AT zhichunzeng paeoniflorininhibitscolorectalcancercellstemnessthroughthemir31945pcateninbetainteractingprotein1axis AT huchen paeoniflorininhibitscolorectalcancercellstemnessthroughthemir31945pcateninbetainteractingprotein1axis AT yuhangguo paeoniflorininhibitscolorectalcancercellstemnessthroughthemir31945pcateninbetainteractingprotein1axis AT yunmao paeoniflorininhibitscolorectalcancercellstemnessthroughthemir31945pcateninbetainteractingprotein1axis AT wencao paeoniflorininhibitscolorectalcancercellstemnessthroughthemir31945pcateninbetainteractingprotein1axis |