Sustainable production of FAEE biodiesel using the oleaginous yeast Yarrowia lipolytica
Abstract Fatty acid ethyl esters (FAEEs) are fatty acid‐derived molecules and serve as an important form of biodiesel. The oleaginous yeast Yarrowia lipolytica is considered an ideal host platform for the production of fatty acid‐derived products due to its excellent lipid accumulation capacity. In...
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| Format: | Article |
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Wiley
2020-07-01
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| Series: | MicrobiologyOpen |
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| Online Access: | https://doi.org/10.1002/mbo3.1051 |
| _version_ | 1811292137122168832 |
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| author | Aiqun Yu Yu Zhao Jian Li Shenglong Li Yaru Pang Yakun Zhao Cuiying Zhang Dongguang Xiao |
| author_facet | Aiqun Yu Yu Zhao Jian Li Shenglong Li Yaru Pang Yakun Zhao Cuiying Zhang Dongguang Xiao |
| author_sort | Aiqun Yu |
| collection | DOAJ |
| description | Abstract Fatty acid ethyl esters (FAEEs) are fatty acid‐derived molecules and serve as an important form of biodiesel. The oleaginous yeast Yarrowia lipolytica is considered an ideal host platform for the production of fatty acid‐derived products due to its excellent lipid accumulation capacity. In this proof‐of‐principle study, several metabolic engineering strategies were applied for the overproduction of FAEE biodiesel in Y. lipolytica. Here, chromosome‐based co‐overexpression of two heterologous genes, namely, PDC1 (encoding pyruvate decarboxylase) and ADH1 (encoding alcohol dehydrogenase) from Saccharomyces cerevisiae, and the endogenous GAPDH (encoding glyceraldehyde‐3‐phosphate dehydrogenase) gene of Y. lipolytica resulted in successful biosynthesis of ethanol at 70.8 mg/L in Y. lipolytica. The engineered Y. lipolytica strain expressing the ethanol synthetic pathway together with a heterologous wax ester synthase (MhWS) exhibited the highest FAEE titer of 360.8 mg/L, which is 3.8‐fold higher than that of the control strain when 2% exogenous ethanol was added to the culture medium of Y. lipolytica. Furthermore, a synthetic microbial consortium comprising an engineered Y. lipolytica strain that heterologously expressed MhWS and a S. cerevisiae strain that could provide ethanol as a substrate for the production of the final product in the final engineered Y. lipolytica strain was created in this study. Finally, this synthetic consortium produced FAEE biodiesel at a titer of 4.8 mg/L under the optimum coculture conditions. |
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| id | doaj.art-ba15b4bf3c0c4e95a4d9c598904e0762 |
| institution | Directory Open Access Journal |
| issn | 2045-8827 |
| language | English |
| last_indexed | 2024-04-13T04:40:45Z |
| publishDate | 2020-07-01 |
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| series | MicrobiologyOpen |
| spelling | doaj.art-ba15b4bf3c0c4e95a4d9c598904e07622022-12-22T03:02:01ZengWileyMicrobiologyOpen2045-88272020-07-0197n/an/a10.1002/mbo3.1051Sustainable production of FAEE biodiesel using the oleaginous yeast Yarrowia lipolyticaAiqun Yu0Yu Zhao1Jian Li2Shenglong Li3Yaru Pang4Yakun Zhao5Cuiying Zhang6Dongguang Xiao7State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education Tianjin Key Laboratory of Industrial Microbiology Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control College of Biotechnology Tianjin University of Science and Technology Tianjin ChinaState Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education Tianjin Key Laboratory of Industrial Microbiology Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control College of Biotechnology Tianjin University of Science and Technology Tianjin ChinaState Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education Tianjin Key Laboratory of Industrial Microbiology Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control College of Biotechnology Tianjin University of Science and Technology Tianjin ChinaState Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education Tianjin Key Laboratory of Industrial Microbiology Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control College of Biotechnology Tianjin University of Science and Technology Tianjin ChinaState Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education Tianjin Key Laboratory of Industrial Microbiology Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control College of Biotechnology Tianjin University of Science and Technology Tianjin ChinaState Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education Tianjin Key Laboratory of Industrial Microbiology Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control College of Biotechnology Tianjin University of Science and Technology Tianjin ChinaState Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education Tianjin Key Laboratory of Industrial Microbiology Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control College of Biotechnology Tianjin University of Science and Technology Tianjin ChinaState Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education Tianjin Key Laboratory of Industrial Microbiology Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control College of Biotechnology Tianjin University of Science and Technology Tianjin ChinaAbstract Fatty acid ethyl esters (FAEEs) are fatty acid‐derived molecules and serve as an important form of biodiesel. The oleaginous yeast Yarrowia lipolytica is considered an ideal host platform for the production of fatty acid‐derived products due to its excellent lipid accumulation capacity. In this proof‐of‐principle study, several metabolic engineering strategies were applied for the overproduction of FAEE biodiesel in Y. lipolytica. Here, chromosome‐based co‐overexpression of two heterologous genes, namely, PDC1 (encoding pyruvate decarboxylase) and ADH1 (encoding alcohol dehydrogenase) from Saccharomyces cerevisiae, and the endogenous GAPDH (encoding glyceraldehyde‐3‐phosphate dehydrogenase) gene of Y. lipolytica resulted in successful biosynthesis of ethanol at 70.8 mg/L in Y. lipolytica. The engineered Y. lipolytica strain expressing the ethanol synthetic pathway together with a heterologous wax ester synthase (MhWS) exhibited the highest FAEE titer of 360.8 mg/L, which is 3.8‐fold higher than that of the control strain when 2% exogenous ethanol was added to the culture medium of Y. lipolytica. Furthermore, a synthetic microbial consortium comprising an engineered Y. lipolytica strain that heterologously expressed MhWS and a S. cerevisiae strain that could provide ethanol as a substrate for the production of the final product in the final engineered Y. lipolytica strain was created in this study. Finally, this synthetic consortium produced FAEE biodiesel at a titer of 4.8 mg/L under the optimum coculture conditions.https://doi.org/10.1002/mbo3.1051biodieselbiosynthesiscocultureFAEEmetabolic engineeringY. lipolytica |
| spellingShingle | Aiqun Yu Yu Zhao Jian Li Shenglong Li Yaru Pang Yakun Zhao Cuiying Zhang Dongguang Xiao Sustainable production of FAEE biodiesel using the oleaginous yeast Yarrowia lipolytica MicrobiologyOpen biodiesel biosynthesis coculture FAEE metabolic engineering Y. lipolytica |
| title | Sustainable production of FAEE biodiesel using the oleaginous yeast Yarrowia lipolytica |
| title_full | Sustainable production of FAEE biodiesel using the oleaginous yeast Yarrowia lipolytica |
| title_fullStr | Sustainable production of FAEE biodiesel using the oleaginous yeast Yarrowia lipolytica |
| title_full_unstemmed | Sustainable production of FAEE biodiesel using the oleaginous yeast Yarrowia lipolytica |
| title_short | Sustainable production of FAEE biodiesel using the oleaginous yeast Yarrowia lipolytica |
| title_sort | sustainable production of faee biodiesel using the oleaginous yeast yarrowia lipolytica |
| topic | biodiesel biosynthesis coculture FAEE metabolic engineering Y. lipolytica |
| url | https://doi.org/10.1002/mbo3.1051 |
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