Supplementation of a single species probiotic does not affect diversity and composition of the healthy adult gastrointestinal microbiome
Over several years, consumer probiotic consumption has increased substantially. Consequently, the number of over-the-counter probiotic products available to consumers has also increased. Many consumers use probiotics for preventative purposes rather than to treat specific illnesses. The influence of...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Human Nutrition & Metabolism |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666149722000111 |
| _version_ | 1817985631384502272 |
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| author | Rachel L. Washburn Daniel Sandberg Michaela A. Gazdik Stofer |
| author_facet | Rachel L. Washburn Daniel Sandberg Michaela A. Gazdik Stofer |
| author_sort | Rachel L. Washburn |
| collection | DOAJ |
| description | Over several years, consumer probiotic consumption has increased substantially. Consequently, the number of over-the-counter probiotic products available to consumers has also increased. Many consumers use probiotics for preventative purposes rather than to treat specific illnesses. The influence of probiotics on the healthy human gut microbiome has not been extensively studied and many questions remain regarding the influence of probiotic supplementation on existing gut flora. In this study, the effect of a commercial probiotic containing Bifidobacterium infantis on the composition and diversity of gut flora in healthy adults was examined. Thirty participants were enrolled in the study and randomly assigned to probiotic (n = 15) or placebo (n = 15) groups. Over the course of the study, three stool samples were collected to facilitate baseline, probiotic/placebo effect, and return to baseline measurements. The probiotic/placebo effect samples were collected after taking a probiotic/placebo tablet daily for 30 days, and the return to baseline sample was collected 30 days after completing the treatment course. V3/V4 16S rRNA sequencing was performed on all samples and data was analyzed using QIIME. No significant difference in gut community diversity or composition between probiotic and placebo groups was observed. This finding suggests that use of a single species probiotic in healthy individuals does not significantly influence microbial gastrointestinal diversity. |
| first_indexed | 2024-04-13T23:59:36Z |
| format | Article |
| id | doaj.art-ba15d44b6b43479f8d0f35c76d8e4a0f |
| institution | Directory Open Access Journal |
| issn | 2666-1497 |
| language | English |
| last_indexed | 2024-04-13T23:59:36Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Human Nutrition & Metabolism |
| spelling | doaj.art-ba15d44b6b43479f8d0f35c76d8e4a0f2022-12-22T02:23:45ZengElsevierHuman Nutrition & Metabolism2666-14972022-06-0128200148Supplementation of a single species probiotic does not affect diversity and composition of the healthy adult gastrointestinal microbiomeRachel L. Washburn0Daniel Sandberg1Michaela A. Gazdik Stofer2Department of Biology, Utah Valley University, Orem, UT, USA; Department of Immunology and Molecular Microbiology, Texas Tech University Health Sciences Center, Lubbock, TX, USADepartment of Biology, Utah Valley University, Orem, UT, USA; College of Medicine - Tucson, University of Arizona, Tucson, AZ, USADepartment of Biology, Utah Valley University, Orem, UT, USA; Corresponding author. 800 West University Parkway Mail Stop 299, Orem, UT, 84058, USA.Over several years, consumer probiotic consumption has increased substantially. Consequently, the number of over-the-counter probiotic products available to consumers has also increased. Many consumers use probiotics for preventative purposes rather than to treat specific illnesses. The influence of probiotics on the healthy human gut microbiome has not been extensively studied and many questions remain regarding the influence of probiotic supplementation on existing gut flora. In this study, the effect of a commercial probiotic containing Bifidobacterium infantis on the composition and diversity of gut flora in healthy adults was examined. Thirty participants were enrolled in the study and randomly assigned to probiotic (n = 15) or placebo (n = 15) groups. Over the course of the study, three stool samples were collected to facilitate baseline, probiotic/placebo effect, and return to baseline measurements. The probiotic/placebo effect samples were collected after taking a probiotic/placebo tablet daily for 30 days, and the return to baseline sample was collected 30 days after completing the treatment course. V3/V4 16S rRNA sequencing was performed on all samples and data was analyzed using QIIME. No significant difference in gut community diversity or composition between probiotic and placebo groups was observed. This finding suggests that use of a single species probiotic in healthy individuals does not significantly influence microbial gastrointestinal diversity.http://www.sciencedirect.com/science/article/pii/S2666149722000111ProbioticsMicrobiome diversityGastrointestinal microbiomeHealthy adults |
| spellingShingle | Rachel L. Washburn Daniel Sandberg Michaela A. Gazdik Stofer Supplementation of a single species probiotic does not affect diversity and composition of the healthy adult gastrointestinal microbiome Human Nutrition & Metabolism Probiotics Microbiome diversity Gastrointestinal microbiome Healthy adults |
| title | Supplementation of a single species probiotic does not affect diversity and composition of the healthy adult gastrointestinal microbiome |
| title_full | Supplementation of a single species probiotic does not affect diversity and composition of the healthy adult gastrointestinal microbiome |
| title_fullStr | Supplementation of a single species probiotic does not affect diversity and composition of the healthy adult gastrointestinal microbiome |
| title_full_unstemmed | Supplementation of a single species probiotic does not affect diversity and composition of the healthy adult gastrointestinal microbiome |
| title_short | Supplementation of a single species probiotic does not affect diversity and composition of the healthy adult gastrointestinal microbiome |
| title_sort | supplementation of a single species probiotic does not affect diversity and composition of the healthy adult gastrointestinal microbiome |
| topic | Probiotics Microbiome diversity Gastrointestinal microbiome Healthy adults |
| url | http://www.sciencedirect.com/science/article/pii/S2666149722000111 |
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