Plasmid-mediated qnrA1 in Klebsiella pneumoniae ST147 in Recife, Brazil
Objectives: Qnr-mediated quinolone resistance is increasingly detected worldwide, but few studies have been carried out so far in Brazil. The aim of this study was to test for qnr determinants in isolates of ciprofloxacin-resistant Klebsiella pneumoniae. Methods: Fifteen ciprofloxacin-resistant isol...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-09-01
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| Series: | International Journal of Infectious Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971214015239 |
| _version_ | 1818915308662947840 |
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| author | Lays Katarina Lima Rocha Rivaldo Lins dos Santos Neto Amanda Cristina da Costa Guimarães Anna Carolina Soares Almeida Marinalda Anselmo Vilela Marcia Maria Camargo de Morais |
| author_facet | Lays Katarina Lima Rocha Rivaldo Lins dos Santos Neto Amanda Cristina da Costa Guimarães Anna Carolina Soares Almeida Marinalda Anselmo Vilela Marcia Maria Camargo de Morais |
| author_sort | Lays Katarina Lima Rocha |
| collection | DOAJ |
| description | Objectives: Qnr-mediated quinolone resistance is increasingly detected worldwide, but few studies have been carried out so far in Brazil. The aim of this study was to test for qnr determinants in isolates of ciprofloxacin-resistant Klebsiella pneumoniae.
Methods: Fifteen ciprofloxacin-resistant isolates from urine cultures of hospitalized patients at a university hospital in North-East Brazil were investigated. Specific PCRs were performed for blaCTX-M and blaTEM, qnr, and class 1 integrons. Plasmid analyses and sequence type (ST) determination were performed, as described previously.
Results: The KP 930 isolate showed qnrA1 and blaTEM-1, together with dfrA12 and aadA2 in a class 1 integron. The qnr gene was located in a 133-kb plasmid. Multilocus sequence typing classified the isolate as ST147.
Conclusions: We identified the combination of qnr with ST147 in Brazil; this is a clone that has disseminated widely and successfully in Latin America. The purpose of describing Qnr-mediated quinolone resistance in North-East Brazil is to draw attention to the spread of this mechanism in the country. |
| first_indexed | 2024-12-20T00:00:13Z |
| format | Article |
| id | doaj.art-ba16b3c6370c4b11b9c963da102e1e34 |
| institution | Directory Open Access Journal |
| issn | 1201-9712 1878-3511 |
| language | English |
| last_indexed | 2024-12-20T00:00:13Z |
| publishDate | 2014-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-ba16b3c6370c4b11b9c963da102e1e342022-12-21T20:00:53ZengElsevierInternational Journal of Infectious Diseases1201-97121878-35112014-09-0126C495010.1016/j.ijid.2014.04.026Plasmid-mediated qnrA1 in Klebsiella pneumoniae ST147 in Recife, BrazilLays Katarina Lima Rocha0Rivaldo Lins dos Santos Neto1Amanda Cristina da Costa Guimarães2Anna Carolina Soares Almeida3Marinalda Anselmo Vilela4Marcia Maria Camargo de Morais5Laboratório de Resistência Microbiana, Instituto de Ciências Biológicas, Universidade de Pernambuco, Recife, BrasilLaboratório de Resistência Microbiana, Instituto de Ciências Biológicas, Universidade de Pernambuco, Recife, BrasilLaboratório de Resistência Microbiana, Instituto de Ciências Biológicas, Universidade de Pernambuco, Recife, BrasilLaboratório de Resistência Microbiana, Instituto de Ciências Biológicas, Universidade de Pernambuco, Recife, BrasilLaboratório de Resistência Microbiana, Instituto de Ciências Biológicas, Universidade de Pernambuco, Recife, BrasilLaboratório de Resistência Microbiana, Instituto de Ciências Biológicas, Universidade de Pernambuco, Recife, BrasilObjectives: Qnr-mediated quinolone resistance is increasingly detected worldwide, but few studies have been carried out so far in Brazil. The aim of this study was to test for qnr determinants in isolates of ciprofloxacin-resistant Klebsiella pneumoniae. Methods: Fifteen ciprofloxacin-resistant isolates from urine cultures of hospitalized patients at a university hospital in North-East Brazil were investigated. Specific PCRs were performed for blaCTX-M and blaTEM, qnr, and class 1 integrons. Plasmid analyses and sequence type (ST) determination were performed, as described previously. Results: The KP 930 isolate showed qnrA1 and blaTEM-1, together with dfrA12 and aadA2 in a class 1 integron. The qnr gene was located in a 133-kb plasmid. Multilocus sequence typing classified the isolate as ST147. Conclusions: We identified the combination of qnr with ST147 in Brazil; this is a clone that has disseminated widely and successfully in Latin America. The purpose of describing Qnr-mediated quinolone resistance in North-East Brazil is to draw attention to the spread of this mechanism in the country.http://www.sciencedirect.com/science/article/pii/S1201971214015239QuinolonesResistanceqnrA1 |
| spellingShingle | Lays Katarina Lima Rocha Rivaldo Lins dos Santos Neto Amanda Cristina da Costa Guimarães Anna Carolina Soares Almeida Marinalda Anselmo Vilela Marcia Maria Camargo de Morais Plasmid-mediated qnrA1 in Klebsiella pneumoniae ST147 in Recife, Brazil International Journal of Infectious Diseases Quinolones Resistance qnrA1 |
| title | Plasmid-mediated qnrA1 in Klebsiella pneumoniae ST147 in Recife, Brazil |
| title_full | Plasmid-mediated qnrA1 in Klebsiella pneumoniae ST147 in Recife, Brazil |
| title_fullStr | Plasmid-mediated qnrA1 in Klebsiella pneumoniae ST147 in Recife, Brazil |
| title_full_unstemmed | Plasmid-mediated qnrA1 in Klebsiella pneumoniae ST147 in Recife, Brazil |
| title_short | Plasmid-mediated qnrA1 in Klebsiella pneumoniae ST147 in Recife, Brazil |
| title_sort | plasmid mediated qnra1 in klebsiella pneumoniae st147 in recife brazil |
| topic | Quinolones Resistance qnrA1 |
| url | http://www.sciencedirect.com/science/article/pii/S1201971214015239 |
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