Bisphenol A induces testicular oxidative stress in mice leading to ferroptosis
Bisphenol A is a common environmental factor and endocrine disruptor that exerts a negative impact on male reproductive ability. By exploring bisphenol A-induced testicular cell death using the Institute of Cancer Research (ICR) mouse model, we found that a ferroptosis phenomenon may exist. Mice wer...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Asian Journal of Andrology |
| Subjects: | |
| Online Access: | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2023;volume=25;issue=3;spage=375;epage=381;aulast= |
| _version_ | 1797824460565250048 |
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| author | Li Li Min-Yan Wang Hua-Bo Jiang Chun-Rong Guo Xian-Dan Zhu Xia-Qin Yao Wei-Wei Zeng Yuan Zhao Ling-Kan Chi |
| author_facet | Li Li Min-Yan Wang Hua-Bo Jiang Chun-Rong Guo Xian-Dan Zhu Xia-Qin Yao Wei-Wei Zeng Yuan Zhao Ling-Kan Chi |
| author_sort | Li Li |
| collection | DOAJ |
| description | Bisphenol A is a common environmental factor and endocrine disruptor that exerts a negative impact on male reproductive ability. By exploring bisphenol A-induced testicular cell death using the Institute of Cancer Research (ICR) mouse model, we found that a ferroptosis phenomenon may exist. Mice were divided into six groups and administered different doses of bisphenol A via intragastric gavage once daily for 45 consecutive days. Serum was then collected to determine the levels of superoxide dismutase and malondialdehyde. Epididymal sperm was also collected for semen analysis, and testicular tissue was collected for ferritin content determination, electron microscope observation of mitochondrial morphology, immunohistochemistry, real-time quantitative polymerase chain reaction, and western blot analysis. Exposure to bisphenol A was found to decrease sperm quality and cause oxidative damage, iron accumulation, and mitochondrial damage in the testes of mice. In addition, bisphenol A was confirmed to affect the expression of the ferroptosis-related genes, glutathione peroxidase 4 (GPX4), ferritin heavy chain 1 (FTH1), cyclooxygenase 2 (COX2), and acyl-CoA synthetase 4 (ACSL4) in mouse testicular tissues. Accordingly, we speculate that bisphenol A induces oxidative stress, which leads to the ferroptosis of testicular cells. Overall, the inhibition of ferroptosis may be a potential strategy to reduce male reproductive toxicity caused by bisphenol A. |
| first_indexed | 2024-03-13T10:39:18Z |
| format | Article |
| id | doaj.art-ba18f4d77eb0454081fd2b158a11766b |
| institution | Directory Open Access Journal |
| issn | 1008-682X 1745-7262 |
| language | English |
| last_indexed | 2024-03-13T10:39:18Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Andrology |
| spelling | doaj.art-ba18f4d77eb0454081fd2b158a11766b2023-05-18T04:59:34ZengWolters Kluwer Medknow PublicationsAsian Journal of Andrology1008-682X1745-72622023-01-0125337538110.4103/aja202266Bisphenol A induces testicular oxidative stress in mice leading to ferroptosisLi LiMin-Yan WangHua-Bo JiangChun-Rong GuoXian-Dan ZhuXia-Qin YaoWei-Wei ZengYuan ZhaoLing-Kan ChiBisphenol A is a common environmental factor and endocrine disruptor that exerts a negative impact on male reproductive ability. By exploring bisphenol A-induced testicular cell death using the Institute of Cancer Research (ICR) mouse model, we found that a ferroptosis phenomenon may exist. Mice were divided into six groups and administered different doses of bisphenol A via intragastric gavage once daily for 45 consecutive days. Serum was then collected to determine the levels of superoxide dismutase and malondialdehyde. Epididymal sperm was also collected for semen analysis, and testicular tissue was collected for ferritin content determination, electron microscope observation of mitochondrial morphology, immunohistochemistry, real-time quantitative polymerase chain reaction, and western blot analysis. Exposure to bisphenol A was found to decrease sperm quality and cause oxidative damage, iron accumulation, and mitochondrial damage in the testes of mice. In addition, bisphenol A was confirmed to affect the expression of the ferroptosis-related genes, glutathione peroxidase 4 (GPX4), ferritin heavy chain 1 (FTH1), cyclooxygenase 2 (COX2), and acyl-CoA synthetase 4 (ACSL4) in mouse testicular tissues. Accordingly, we speculate that bisphenol A induces oxidative stress, which leads to the ferroptosis of testicular cells. Overall, the inhibition of ferroptosis may be a potential strategy to reduce male reproductive toxicity caused by bisphenol A.http://www.ajandrology.com/article.asp?issn=1008-682X;year=2023;volume=25;issue=3;spage=375;epage=381;aulast=bisphenol a; ferroptosis; mitochondrial damage; oxidative stress; testicular toxicity |
| spellingShingle | Li Li Min-Yan Wang Hua-Bo Jiang Chun-Rong Guo Xian-Dan Zhu Xia-Qin Yao Wei-Wei Zeng Yuan Zhao Ling-Kan Chi Bisphenol A induces testicular oxidative stress in mice leading to ferroptosis Asian Journal of Andrology bisphenol a; ferroptosis; mitochondrial damage; oxidative stress; testicular toxicity |
| title | Bisphenol A induces testicular oxidative stress in mice leading to ferroptosis |
| title_full | Bisphenol A induces testicular oxidative stress in mice leading to ferroptosis |
| title_fullStr | Bisphenol A induces testicular oxidative stress in mice leading to ferroptosis |
| title_full_unstemmed | Bisphenol A induces testicular oxidative stress in mice leading to ferroptosis |
| title_short | Bisphenol A induces testicular oxidative stress in mice leading to ferroptosis |
| title_sort | bisphenol a induces testicular oxidative stress in mice leading to ferroptosis |
| topic | bisphenol a; ferroptosis; mitochondrial damage; oxidative stress; testicular toxicity |
| url | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2023;volume=25;issue=3;spage=375;epage=381;aulast= |
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