Evaluation of Foveal Cone and Müller Cells in Epiretinal Membrane using Adaptive Optics OCT
Objective: To investigate cellular-level morphological alterations in the retinal neuroglia in eyes with epiretinal membrane (ERM). Design: Prospective cross-sectional, observational study (November 2020–May 2022). Subjects and Controls: We included 41 eyes with unilateral idiopathic ERM and 33 heal...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Ophthalmology Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666914523000945 |
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| author | Masaharu Ishikura, MD Yuki Muraoka, MD, PhD Shin Kadomoto, MD, PhD Naomi Nishigori, MD Takahiro Kogo, MD Shogo Numa, MD, PhD Eri Nakano, MD, PhD Masayuki Hata, MD, PhD Kenji Ishihara, MD, PhD Sotaro Ooto, MD, PhD Akitaka Tsujikawa, MD, PhD |
| author_facet | Masaharu Ishikura, MD Yuki Muraoka, MD, PhD Shin Kadomoto, MD, PhD Naomi Nishigori, MD Takahiro Kogo, MD Shogo Numa, MD, PhD Eri Nakano, MD, PhD Masayuki Hata, MD, PhD Kenji Ishihara, MD, PhD Sotaro Ooto, MD, PhD Akitaka Tsujikawa, MD, PhD |
| author_sort | Masaharu Ishikura, MD |
| collection | DOAJ |
| description | Objective: To investigate cellular-level morphological alterations in the retinal neuroglia in eyes with epiretinal membrane (ERM). Design: Prospective cross-sectional, observational study (November 2020–May 2022). Subjects and Controls: We included 41 eyes with unilateral idiopathic ERM and 33 healthy eyes of healthy volunteers. Methods: We examined the foveal microstructures in all eyes using adaptive optics OCT (AO-OCT) with axial and lateral resolutions of 3.4 and 3.0 μm, respectively. Adaptive optics OCT images were acquired for a 2.5° (728 μm) area at the foveal center. Main Outcome Measures: Foveal microstructures on AO-OCT images, best-corrected visual acuity (BCVA) in logarithm of the minimum angle of resolution units, and associations between these parameters. Results: Adaptive optics OCT imaging of healthy eyes and eyes with ERM revealed sharp hyperreflective lines of the external limiting membrane (ELM), accompanied by hyporeflective gaps, individual nuclei of the foveal cone photoreceptors, and Müller cell bodies. The arrangement of Müller cell bodies was more vertical in eyes with ERM than in normal eyes. Epiretinal membranes adhered to foveal Müller cells via the internal limiting membrane (ILM), exerting vertical traction that pulled the foveal cones anteriorly. Adaptive optics OCT also enabled visualization of outer segment (OS) discs. Hyperreflective changes in the OS discs were observed beneath the vertically thickened ellipsoid zone (EZ) in 15 eyes (36.6%) with ERM. For eyes with ERM, multiple regression analysis showed that the length from ILM to the inner border of the outer nuclear layer and the EZ thickness were significantly associated with BCVA (β = 5.3 × 10-4 and 82.7 × 10-4, respectively), with associated 95% confidence intervals of 1.3 × 10-4 to 9.3 × 10-4 (P = 0.011) and 39.0 × 10-4 to 126.5 × 10-4 (P < 0.001), respectively. The EZ thickness was significantly and positively associated with the length from ELM to the retinal pigment epithelium (β = 23.9 × 10-2, 95% confidence interval: 4.8 × 10-2 to 42.9 × 10-2; P = 0.015). Conclusions: Cellular imaging of retinal neuroglia by AO-OCT may suggest possible mechanisms associated with visual impairment in patients with ERM, which could potentially contribute to the growing body of knowledge on its pathophysiology. However, these insights require further validation through extensive studies to fully ascertain their significance. Financial Disclosure(s): Proprietary or commercial disclosure may be found in the Footnotes and Disclosures at the end of this article. |
| first_indexed | 2024-03-11T18:07:36Z |
| format | Article |
| id | doaj.art-ba194ad30eed4b33b086c47c042c8e56 |
| institution | Directory Open Access Journal |
| issn | 2666-9145 |
| language | English |
| last_indexed | 2024-03-11T18:07:36Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ophthalmology Science |
| spelling | doaj.art-ba194ad30eed4b33b086c47c042c8e562023-10-17T04:07:28ZengElsevierOphthalmology Science2666-91452024-01-0141100362Evaluation of Foveal Cone and Müller Cells in Epiretinal Membrane using Adaptive Optics OCTMasaharu Ishikura, MD0Yuki Muraoka, MD, PhD1Shin Kadomoto, MD, PhD2Naomi Nishigori, MD3Takahiro Kogo, MD4Shogo Numa, MD, PhD5Eri Nakano, MD, PhD6Masayuki Hata, MD, PhD7Kenji Ishihara, MD, PhD8Sotaro Ooto, MD, PhD9Akitaka Tsujikawa, MD, PhD10Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, JapanCorrespondence: Yuki Muraoka, MD, PhD, Department of Ophthalmology, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto 606-8507, Japan.; Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, JapanDepartment of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, JapanDepartment of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, JapanDepartment of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, JapanDepartment of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, JapanDepartment of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, JapanDepartment of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, JapanDepartment of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, JapanDepartment of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, JapanDepartment of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, JapanObjective: To investigate cellular-level morphological alterations in the retinal neuroglia in eyes with epiretinal membrane (ERM). Design: Prospective cross-sectional, observational study (November 2020–May 2022). Subjects and Controls: We included 41 eyes with unilateral idiopathic ERM and 33 healthy eyes of healthy volunteers. Methods: We examined the foveal microstructures in all eyes using adaptive optics OCT (AO-OCT) with axial and lateral resolutions of 3.4 and 3.0 μm, respectively. Adaptive optics OCT images were acquired for a 2.5° (728 μm) area at the foveal center. Main Outcome Measures: Foveal microstructures on AO-OCT images, best-corrected visual acuity (BCVA) in logarithm of the minimum angle of resolution units, and associations between these parameters. Results: Adaptive optics OCT imaging of healthy eyes and eyes with ERM revealed sharp hyperreflective lines of the external limiting membrane (ELM), accompanied by hyporeflective gaps, individual nuclei of the foveal cone photoreceptors, and Müller cell bodies. The arrangement of Müller cell bodies was more vertical in eyes with ERM than in normal eyes. Epiretinal membranes adhered to foveal Müller cells via the internal limiting membrane (ILM), exerting vertical traction that pulled the foveal cones anteriorly. Adaptive optics OCT also enabled visualization of outer segment (OS) discs. Hyperreflective changes in the OS discs were observed beneath the vertically thickened ellipsoid zone (EZ) in 15 eyes (36.6%) with ERM. For eyes with ERM, multiple regression analysis showed that the length from ILM to the inner border of the outer nuclear layer and the EZ thickness were significantly associated with BCVA (β = 5.3 × 10-4 and 82.7 × 10-4, respectively), with associated 95% confidence intervals of 1.3 × 10-4 to 9.3 × 10-4 (P = 0.011) and 39.0 × 10-4 to 126.5 × 10-4 (P < 0.001), respectively. The EZ thickness was significantly and positively associated with the length from ELM to the retinal pigment epithelium (β = 23.9 × 10-2, 95% confidence interval: 4.8 × 10-2 to 42.9 × 10-2; P = 0.015). Conclusions: Cellular imaging of retinal neuroglia by AO-OCT may suggest possible mechanisms associated with visual impairment in patients with ERM, which could potentially contribute to the growing body of knowledge on its pathophysiology. However, these insights require further validation through extensive studies to fully ascertain their significance. Financial Disclosure(s): Proprietary or commercial disclosure may be found in the Footnotes and Disclosures at the end of this article.http://www.sciencedirect.com/science/article/pii/S2666914523000945Epiretinal membraneAdaptive opticsAdaptive optics optical coherence tomographyMüller cellEllipsoid zone |
| spellingShingle | Masaharu Ishikura, MD Yuki Muraoka, MD, PhD Shin Kadomoto, MD, PhD Naomi Nishigori, MD Takahiro Kogo, MD Shogo Numa, MD, PhD Eri Nakano, MD, PhD Masayuki Hata, MD, PhD Kenji Ishihara, MD, PhD Sotaro Ooto, MD, PhD Akitaka Tsujikawa, MD, PhD Evaluation of Foveal Cone and Müller Cells in Epiretinal Membrane using Adaptive Optics OCT Ophthalmology Science Epiretinal membrane Adaptive optics Adaptive optics optical coherence tomography Müller cell Ellipsoid zone |
| title | Evaluation of Foveal Cone and Müller Cells in Epiretinal Membrane using Adaptive Optics OCT |
| title_full | Evaluation of Foveal Cone and Müller Cells in Epiretinal Membrane using Adaptive Optics OCT |
| title_fullStr | Evaluation of Foveal Cone and Müller Cells in Epiretinal Membrane using Adaptive Optics OCT |
| title_full_unstemmed | Evaluation of Foveal Cone and Müller Cells in Epiretinal Membrane using Adaptive Optics OCT |
| title_short | Evaluation of Foveal Cone and Müller Cells in Epiretinal Membrane using Adaptive Optics OCT |
| title_sort | evaluation of foveal cone and muller cells in epiretinal membrane using adaptive optics oct |
| topic | Epiretinal membrane Adaptive optics Adaptive optics optical coherence tomography Müller cell Ellipsoid zone |
| url | http://www.sciencedirect.com/science/article/pii/S2666914523000945 |
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