Enhancement of chemopreventive properties of young green barley and chlorella extracts used together against colon cancer cells
Introduction and objective Young green barley and chlorella are listed as a panacea for a whole range of disorders, including diabetes, cardiovascular disease, infections, and hypercholesterolaemia. The first reports have appeared presenting the anticancer properties of these products. The present s...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Institute of Rural Health
2020-12-01
|
| Series: | Annals of Agricultural and Environmental Medicine |
| Subjects: | |
| Online Access: | http://www.aaem.pl/Enhancement-of-chemopreventive-properties-of-young-green-barley-and-chlorella-extracts,130555,0,2.html |
| _version_ | 1818612918558654464 |
|---|---|
| author | Marta Kinga Lemieszek Wojciech Rzeski |
| author_facet | Marta Kinga Lemieszek Wojciech Rzeski |
| author_sort | Marta Kinga Lemieszek |
| collection | DOAJ |
| description | Introduction and objective
Young green barley and chlorella are listed as a panacea for a whole range of disorders, including diabetes, cardiovascular disease, infections, and hypercholesterolaemia. The first reports have appeared presenting the anticancer properties of these products. The present study is an attempt to extend this knowledge with particular emphasis on the possibility of using young green barley, chlorella, and their combination in colon cancer chemoprevention.
Material and methods
Extracts of young green barley (YB) and chlorella (CH), as well as their combination MIX (YB+CH; 1:1), were examined. The influence of the extracts on viability and proliferation of human colon epithelial CCD841 CoN cells was analyzed by LDH and MTT assays. Anticancer properties of extracts were screened on human colon adenocarcinoma cell line HT-29 by MTT and BrdU assays. Changes in cells morphology in response to extracts were investigated after May-Grünwald-Giemsa staining.
Results
Extracts used together or separately did not affect the viability and proliferation of CCD841 CoN cells. Simultaneously, YB, CH and MIX inhibited proliferation of HT-29 cells in a dose-dependent manner. Furthermore changes in the morphology of HT-29 cells treated with YB, CH and MIX suggested necrosis induction. Performed studies also revealed that MIX induced a stronger antiproliferative effect than their individual ingredients; however, the discovered enhancement of anticancer properties was weaker than the sum of the effects of YB and CH creating MIX.
Conclusions
The study demonstrates great chemopreventive properties of young green barley and chlorella water extracts and their combination in in vitro model of colon cancer. The anticancer properties of the investigated extracts were significantly enhanced after combining, without an increase of their toxicity against normal cells |
| first_indexed | 2024-12-16T15:53:51Z |
| format | Article |
| id | doaj.art-ba1abc7d70e843788021c27a772d0cfc |
| institution | Directory Open Access Journal |
| issn | 1232-1966 1898-2263 |
| language | English |
| last_indexed | 2024-12-16T15:53:51Z |
| publishDate | 2020-12-01 |
| publisher | Institute of Rural Health |
| record_format | Article |
| series | Annals of Agricultural and Environmental Medicine |
| spelling | doaj.art-ba1abc7d70e843788021c27a772d0cfc2022-12-21T22:25:38ZengInstitute of Rural HealthAnnals of Agricultural and Environmental Medicine1232-19661898-22632020-12-0127459159810.26444/aaem/130555130555Enhancement of chemopreventive properties of young green barley and chlorella extracts used together against colon cancer cellsMarta Kinga Lemieszek0Wojciech Rzeski1Department of Medical Biology, Institute of Rural Health, Lublin, PolandDepartment of Medical Biology, Institute of Rural Health, Lublin, PolandIntroduction and objective Young green barley and chlorella are listed as a panacea for a whole range of disorders, including diabetes, cardiovascular disease, infections, and hypercholesterolaemia. The first reports have appeared presenting the anticancer properties of these products. The present study is an attempt to extend this knowledge with particular emphasis on the possibility of using young green barley, chlorella, and their combination in colon cancer chemoprevention. Material and methods Extracts of young green barley (YB) and chlorella (CH), as well as their combination MIX (YB+CH; 1:1), were examined. The influence of the extracts on viability and proliferation of human colon epithelial CCD841 CoN cells was analyzed by LDH and MTT assays. Anticancer properties of extracts were screened on human colon adenocarcinoma cell line HT-29 by MTT and BrdU assays. Changes in cells morphology in response to extracts were investigated after May-Grünwald-Giemsa staining. Results Extracts used together or separately did not affect the viability and proliferation of CCD841 CoN cells. Simultaneously, YB, CH and MIX inhibited proliferation of HT-29 cells in a dose-dependent manner. Furthermore changes in the morphology of HT-29 cells treated with YB, CH and MIX suggested necrosis induction. Performed studies also revealed that MIX induced a stronger antiproliferative effect than their individual ingredients; however, the discovered enhancement of anticancer properties was weaker than the sum of the effects of YB and CH creating MIX. Conclusions The study demonstrates great chemopreventive properties of young green barley and chlorella water extracts and their combination in in vitro model of colon cancer. The anticancer properties of the investigated extracts were significantly enhanced after combining, without an increase of their toxicity against normal cellshttp://www.aaem.pl/Enhancement-of-chemopreventive-properties-of-young-green-barley-and-chlorella-extracts,130555,0,2.htmlhordeum vulgarechlorella pyrenoidosasuper-foodfunctional foodchemopreventioncolon cancer |
| spellingShingle | Marta Kinga Lemieszek Wojciech Rzeski Enhancement of chemopreventive properties of young green barley and chlorella extracts used together against colon cancer cells Annals of Agricultural and Environmental Medicine hordeum vulgare chlorella pyrenoidosa super-food functional food chemoprevention colon cancer |
| title | Enhancement of chemopreventive properties of young green barley and chlorella extracts used together against colon cancer cells |
| title_full | Enhancement of chemopreventive properties of young green barley and chlorella extracts used together against colon cancer cells |
| title_fullStr | Enhancement of chemopreventive properties of young green barley and chlorella extracts used together against colon cancer cells |
| title_full_unstemmed | Enhancement of chemopreventive properties of young green barley and chlorella extracts used together against colon cancer cells |
| title_short | Enhancement of chemopreventive properties of young green barley and chlorella extracts used together against colon cancer cells |
| title_sort | enhancement of chemopreventive properties of young green barley and chlorella extracts used together against colon cancer cells |
| topic | hordeum vulgare chlorella pyrenoidosa super-food functional food chemoprevention colon cancer |
| url | http://www.aaem.pl/Enhancement-of-chemopreventive-properties-of-young-green-barley-and-chlorella-extracts,130555,0,2.html |
| work_keys_str_mv | AT martakingalemieszek enhancementofchemopreventivepropertiesofyounggreenbarleyandchlorellaextractsusedtogetheragainstcoloncancercells AT wojciechrzeski enhancementofchemopreventivepropertiesofyounggreenbarleyandchlorellaextractsusedtogetheragainstcoloncancercells |