Extraction, Isolation, and Component Analysis of Turmeric-Derived Exosome-like Nanoparticles
As one kind of plant-derived extracellular vesicle, turmeric-derived exosome-like nanoparticles (TELNs) are composed of proteins, lipids, nucleic acids, and small-molecule compounds, which possess good biocompatibility and safety. They are especially rich in information from the “mother plant”, whic...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/10/10/1199 |
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| author | Yongsheng Wei Xiang Cai Qiqi Wu Hui Liao Shuang Liang Hongwei Fu Qi Xiang Shu Zhang |
| author_facet | Yongsheng Wei Xiang Cai Qiqi Wu Hui Liao Shuang Liang Hongwei Fu Qi Xiang Shu Zhang |
| author_sort | Yongsheng Wei |
| collection | DOAJ |
| description | As one kind of plant-derived extracellular vesicle, turmeric-derived exosome-like nanoparticles (TELNs) are composed of proteins, lipids, nucleic acids, and small-molecule compounds, which possess good biocompatibility and safety. They are especially rich in information from the “mother plant”, which provides more applications in biological fields. In this study, we isolated and purified TELNs using differential centrifugation and ultracentrifugation and systematically detected their physicochemical properties using multi-omics. The TELNs possessed a typical teacup-like exosome morphology, and the extraction rate was approximately 1.71 ± 0.176 mg/g. The average particle size was 183.2 ± 10.9 nm, and the average zeta potential was −17.6 ± 1.19 mV. They were rich in lipids, mainly phosphatidylethanolamine (PE) (17.4%), triglyceride (TG) (12.3%), phosphatidylinositol (PI) (9.82%), and phosphatidylcholine (PC) (7.93%). All of them are the key lipids in the exosomes. The protein content was approximately 12% (M/M), mainly curcumin synthase and other proteins involved in secondary metabolite biosynthesis. In addition, there are critical essential genes for curcumin biosynthesis, such as curcumin synthase (CURS) and diketocoenzyme A synthase (DCS). More importantly, a greater variety of small-molecule compounds, primarily curcumin and curcumin analogs such as demethoxycurcumin and volatile oleoresins such as curcuminoids, have now been revealed. In conclusion, TELNs were successfully isolated, containing 0.17% (M/M) turmeric and a large amount of chemical information, the same as the parent-of-origin plant. This is the first time combining multi-omics to analyze the characteristics and nature of the TELNs, which laid a solid material foundation for the further development of turmeric. |
| first_indexed | 2024-03-10T21:25:48Z |
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| language | English |
| last_indexed | 2024-03-10T21:25:48Z |
| publishDate | 2023-10-01 |
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| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-ba1ca87ff7eb4093897844d42dbb9a272023-11-19T15:42:24ZengMDPI AGBioengineering2306-53542023-10-011010119910.3390/bioengineering10101199Extraction, Isolation, and Component Analysis of Turmeric-Derived Exosome-like NanoparticlesYongsheng Wei0Xiang Cai1Qiqi Wu2Hui Liao3Shuang Liang4Hongwei Fu5Qi Xiang6Shu Zhang7Guangdong Provincial Key Laboratory of Advanced Drug Delivery Systems, Center for Drug Research and Development of Guangdong Pharmaceutical University, Guangzhou 510006, ChinaBiopharmaceutical R&D Center of Jinan University Co., Ltd., Guangzhou 510632, ChinaBiopharmaceutical R&D Center of Jinan University Co., Ltd., Guangzhou 510632, ChinaBiopharmaceutical R&D Center of Jinan University Co., Ltd., Guangzhou 510632, ChinaBiopharmaceutical R&D Center of Jinan University Co., Ltd., Guangzhou 510632, ChinaGuangdong Provincial Key Laboratory of Advanced Drug Delivery Systems, Center for Drug Research and Development of Guangdong Pharmaceutical University, Guangzhou 510006, ChinaBiopharmaceutical R&D Center of Jinan University Co., Ltd., Guangzhou 510632, ChinaGuangdong Provincial Key Laboratory of Advanced Drug Delivery Systems, Center for Drug Research and Development of Guangdong Pharmaceutical University, Guangzhou 510006, ChinaAs one kind of plant-derived extracellular vesicle, turmeric-derived exosome-like nanoparticles (TELNs) are composed of proteins, lipids, nucleic acids, and small-molecule compounds, which possess good biocompatibility and safety. They are especially rich in information from the “mother plant”, which provides more applications in biological fields. In this study, we isolated and purified TELNs using differential centrifugation and ultracentrifugation and systematically detected their physicochemical properties using multi-omics. The TELNs possessed a typical teacup-like exosome morphology, and the extraction rate was approximately 1.71 ± 0.176 mg/g. The average particle size was 183.2 ± 10.9 nm, and the average zeta potential was −17.6 ± 1.19 mV. They were rich in lipids, mainly phosphatidylethanolamine (PE) (17.4%), triglyceride (TG) (12.3%), phosphatidylinositol (PI) (9.82%), and phosphatidylcholine (PC) (7.93%). All of them are the key lipids in the exosomes. The protein content was approximately 12% (M/M), mainly curcumin synthase and other proteins involved in secondary metabolite biosynthesis. In addition, there are critical essential genes for curcumin biosynthesis, such as curcumin synthase (CURS) and diketocoenzyme A synthase (DCS). More importantly, a greater variety of small-molecule compounds, primarily curcumin and curcumin analogs such as demethoxycurcumin and volatile oleoresins such as curcuminoids, have now been revealed. In conclusion, TELNs were successfully isolated, containing 0.17% (M/M) turmeric and a large amount of chemical information, the same as the parent-of-origin plant. This is the first time combining multi-omics to analyze the characteristics and nature of the TELNs, which laid a solid material foundation for the further development of turmeric.https://www.mdpi.com/2306-5354/10/10/1199turmeric-derived exosome-like nanoparticles (TELNs)multi-omicscomponent analysis |
| spellingShingle | Yongsheng Wei Xiang Cai Qiqi Wu Hui Liao Shuang Liang Hongwei Fu Qi Xiang Shu Zhang Extraction, Isolation, and Component Analysis of Turmeric-Derived Exosome-like Nanoparticles Bioengineering turmeric-derived exosome-like nanoparticles (TELNs) multi-omics component analysis |
| title | Extraction, Isolation, and Component Analysis of Turmeric-Derived Exosome-like Nanoparticles |
| title_full | Extraction, Isolation, and Component Analysis of Turmeric-Derived Exosome-like Nanoparticles |
| title_fullStr | Extraction, Isolation, and Component Analysis of Turmeric-Derived Exosome-like Nanoparticles |
| title_full_unstemmed | Extraction, Isolation, and Component Analysis of Turmeric-Derived Exosome-like Nanoparticles |
| title_short | Extraction, Isolation, and Component Analysis of Turmeric-Derived Exosome-like Nanoparticles |
| title_sort | extraction isolation and component analysis of turmeric derived exosome like nanoparticles |
| topic | turmeric-derived exosome-like nanoparticles (TELNs) multi-omics component analysis |
| url | https://www.mdpi.com/2306-5354/10/10/1199 |
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