Application of FTA® Cards for detection and storage of avian influenza virus
Introduction: The aim of the study was to test the utility of Flinders Technology Associates filter paper (FTA® Cards) for molecular detection and storage of avian influenza virus (AIV). Material and Methods: There were two strains of AIV used in the study: low pathogenicity H7N1 and high pathogenic...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2016-03-01
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| Series: | Journal of Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/jvetres-2016-0001 |
| _version_ | 1818677568029589504 |
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| author | Jóźwiak Michał Wyrostek Krzysztof Domańska-Blicharz Katarzyna Olszewska-Tomczyk Monika Śmietanka Krzysztof Minta Zenon |
| author_facet | Jóźwiak Michał Wyrostek Krzysztof Domańska-Blicharz Katarzyna Olszewska-Tomczyk Monika Śmietanka Krzysztof Minta Zenon |
| author_sort | Jóźwiak Michał |
| collection | DOAJ |
| description | Introduction: The aim of the study was to test the utility of Flinders Technology Associates filter paper (FTA® Cards) for molecular detection and storage of avian influenza virus (AIV). Material and Methods: There were two strains of AIV used in the study: low pathogenicity H7N1 and high pathogenicity H5N1 subtypes. Detection of viral material was conducted using molecular RT-PCR and rRT- PCR method. Results: The infectivity of LPAIV/H7N1 and HPAIV/H5N1 was completely inactivated within 1 h and 24 h after adsorption to FTA® Cards at room temperature, respectively. Viruses stored on FTA® Cards had detection limit approximately 1 log10 lower than live viruses. Viral RNA of both strains were detectable on the cards by rRT-PCR for a minimum of 150 d, irrespectively of storage temperatures (room temperature, -20ºC). RNA was also detected in all samples obtained from SPF chickens experimentally infected with HPAI/H5N1 on 3rd and 4th day post-infection (p.i.). |
| first_indexed | 2024-12-17T09:01:26Z |
| format | Article |
| id | doaj.art-ba1f83e65b7143a1a321966d31ae8fca |
| institution | Directory Open Access Journal |
| issn | 2450-8608 |
| language | English |
| last_indexed | 2024-12-17T09:01:26Z |
| publishDate | 2016-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Journal of Veterinary Research |
| spelling | doaj.art-ba1f83e65b7143a1a321966d31ae8fca2022-12-21T21:55:42ZengSciendoJournal of Veterinary Research2450-86082016-03-016011610.1515/jvetres-2016-0001jvetres-2016-0001Application of FTA® Cards for detection and storage of avian influenza virusJóźwiak Michał0Wyrostek Krzysztof1Domańska-Blicharz Katarzyna2Olszewska-Tomczyk Monika3Śmietanka Krzysztof4Minta Zenon5Department of Poultry Diseases, National Veterinary Research Institute, 24-100 Pulawy, PolandDepartment of Poultry Diseases, National Veterinary Research Institute, 24-100 Pulawy, PolandDepartment of Poultry Diseases, National Veterinary Research Institute, 24-100 Pulawy, PolandDepartment of Poultry Diseases, National Veterinary Research Institute, 24-100 Pulawy, PolandDepartment of Poultry Diseases, National Veterinary Research Institute, 24-100 Pulawy, PolandDepartment of Poultry Diseases, National Veterinary Research Institute, 24-100 Pulawy, PolandIntroduction: The aim of the study was to test the utility of Flinders Technology Associates filter paper (FTA® Cards) for molecular detection and storage of avian influenza virus (AIV). Material and Methods: There were two strains of AIV used in the study: low pathogenicity H7N1 and high pathogenicity H5N1 subtypes. Detection of viral material was conducted using molecular RT-PCR and rRT- PCR method. Results: The infectivity of LPAIV/H7N1 and HPAIV/H5N1 was completely inactivated within 1 h and 24 h after adsorption to FTA® Cards at room temperature, respectively. Viruses stored on FTA® Cards had detection limit approximately 1 log10 lower than live viruses. Viral RNA of both strains were detectable on the cards by rRT-PCR for a minimum of 150 d, irrespectively of storage temperatures (room temperature, -20ºC). RNA was also detected in all samples obtained from SPF chickens experimentally infected with HPAI/H5N1 on 3rd and 4th day post-infection (p.i.).https://doi.org/10.1515/jvetres-2016-0001avian influenza virusfta® cardsmolecular detectionstorage |
| spellingShingle | Jóźwiak Michał Wyrostek Krzysztof Domańska-Blicharz Katarzyna Olszewska-Tomczyk Monika Śmietanka Krzysztof Minta Zenon Application of FTA® Cards for detection and storage of avian influenza virus Journal of Veterinary Research avian influenza virus fta® cards molecular detection storage |
| title | Application of FTA® Cards for detection and storage of avian influenza virus |
| title_full | Application of FTA® Cards for detection and storage of avian influenza virus |
| title_fullStr | Application of FTA® Cards for detection and storage of avian influenza virus |
| title_full_unstemmed | Application of FTA® Cards for detection and storage of avian influenza virus |
| title_short | Application of FTA® Cards for detection and storage of avian influenza virus |
| title_sort | application of fta r cards for detection and storage of avian influenza virus |
| topic | avian influenza virus fta® cards molecular detection storage |
| url | https://doi.org/10.1515/jvetres-2016-0001 |
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